Comparative antitumor and anti-proliferative activities of Hippophae rhamnoides L. leaves extracts

作     者：Javid Ali Bashir Ahmad 

作者机构：PCSIR Laboratories ComplexJamrude Road PeshawarKPK-Pakistan Center of Biotechnology and MicrobiologyUniversity of PeshawarKPK-Pakistan 

出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)

年 卷 期：2015年第3卷第3期

页      面：228-232页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

主　　题：Seabuckthorn Solvent extracts Tumor inhibition Anti-cancer Potato disc assay HeLa cell line 

摘      要：Objective:To evaluate the antitumor and anti-proliferative activities of methanol,aqueous,acetone,ethyl acetate,ethanol,chloroform and n-hexane extracts of Hippophae rhamnoides ***:Antitumor activities were evaluated by using the antitumor potato disc assay by using inoculums(Agrobacterium tumefaciens)with three different concentrations of test samples(10,100 and 1000 mg/L).Anti-proliferative activity was evaluated by the given method of methyl thiazolyl tetrazolium *** concentrations of the extract ranging from 0.039 to 10 mg/mL were tested against HeLa ***:Highest tumors inhibition activity(60.9%and 55.8%)was shown by methanol and ethanol extracts,with EC_(50) values of 424.41 and 434.61 mg/L *** 10 mg/mL,The highest cell inhibition 75.61%was observed in methanol extract and the lowest 36.59%were calculated in n-hexane *** difference in tumor and cell inhibition(%)may be due to the different concentration of active compounds responsible for antitumor and anti-proliferative *** extracts have considerable level of tumor and cell inhibitiory effect in a dose dependent ***:Our finding showed that Hippophae rhamnoides leaves are a potent natural source of antitumor and antiproliferative agent.