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Journal of Pharmaceutical Analysis 2021年第5期11卷 646-652页

作者： Elsa Maria Materon Ademar Wong Tayane Aguiar Freitas Ronaldo Censi Faria Osvaldo N.Oliveira Jr. Department of Chemistry Federal University of Sao Carlos13560-970Sao CarlosBrazil Sao Carlos Institute of Physics University of Sao Paulo13560-970Sao CarlosBrazil

Monitoring the concentration of antibiotics in body fluids is essential to optimizing the therapy and minimizing the risk of bacteria resistance,which can be made with electrochemical sensors tailored with appropriate *** this paper,we report on sensors made with screen-printed electrodes(SPE)coated with fullerene(C60),reduced graphene oxide(rGO)and Nafion(NF)(C60-rGO-NF/SPE)to determine the antibiotic metronidazole(MTZ).Under optimized conditions,the C60-rGO-NF/SPE sensor exhibited a linear response in square wave voltammetry for MTZ concentrations from 2.5×10^(-7) to 34×10^(-6) mol/L,with a detection limit of 2.1×10^(-7) mol/*** sensor was also capable of detecting MTZ in serum and urine,with recovery between 94%and 100%,which are similar to those of the standard chromatographic method(HPLC-UV).Because the C60-rGO-NF/SPE sensor is amenable to mass production and allows for MTZ determination with simple principles of detection,it fulfills the requirements of therapeutic drug monitoring programs.

关键词： Metronidazole Fullerene Reduced graphene oxide screen-printed electrodes Antibiotic

A screen-printed, amperometric biosensor for the determination of organophosphorus pesticides in water samples

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Journal of Environmental Sciences 2012年第5期24卷 956-962页

作者： Raju Sekar Department of Biological Sciences Xi'an Jiaotong-Liverpool University

An amperometric biosensor based on screen-printed electrodes （SPEs） was developed for the determination of organophosphorus pesticides in water samples. The extent of acetylcholinesterase （AChE） deactivation was determined and quantified for pesticide concentrations in water samples. An enzyme immobilization adsorption procedure and polyacrylamide gel matrix polymerization were used for fabrication of the biosensor, with minimal losses in enzyme activity. The optimal conditions for enzyme catalytic reaction on the SPEs surfaces were acetylthiocholine chloride （ATChCl） concentration of 5 mmol/L, pH 7 and reaction time of 4 min. The detection limits for three organophosphorus pesticides （dichlorvos, monocrotophs and parathion） were in the range of 4 to 7 μg/L when an AChE amount of 0.1 U was used for immobilization.

关键词： organophosphorus pesticides acetylcholinesterase enzyme immobilization screen-printed electrodes biosensor

Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

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Frontiers of Environmental Science & Engineering 2012年第6期6卷 831-838页

作者： Yuanting LI Dawei LI Wei SONG Meng LI Jie ZOU Yitao LONG Key Laboratory for Advanced Materials&Department of Chemistry East China University of Science and TechnologyShanghai 200237China Jiangsu Provincial Supervising&Testing Research Institute for Products Quality Nanjing 210007China

A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase （SWCNTs-AuNPs-Tyr） modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron micro- scopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase （Tyr） with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8.0 × 10^-8 to 2.0 × 10^- 5 mol.L-1 with a detection limit of 4.5 × 10^-8 mol.L-1 （S/N= 3）. This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosen- sor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.

关键词： on-site determination tyrosinase biosensor,phenolic contaminants single-walled carbon nanotubes,gold nanoparticles screen-printed electrodes

Noninvasive Label-Free Detection of Cortisol and Lactate Using Graphene Embedded screen-printed Electrode

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Nano-Micro Letters 2018年第3期10卷 42-51页

作者： Satish K.Tuteja Connor Ormsby Suresh Neethirajan BioNano Lab School of Engineering University of Guelph

A sensitive and specific immunosensor for the detection of the hormones cortisol and lactate in human or animal biological fluids, such as sweat and saliva, was devised using the label-free electrochemical chronoamperometric technique. By using these fluids instead of blood,the biosensor becomes noninvasive and is less stressful to the end user, who may be a small child or a farm *** graphene oxide(e-RGO) was used as a synergistic platform for signal amplification and template for bioconjugation for the sensing mechanism on a screenprinted electrode. The cortisol and lactate antibodies were bioconjugated to the e-RGO using covalent carbodiimide chemistry. Label-free electrochemical chronoamperometric detection was used to analyze the response to the desired biomolecules over the wide detection range. A detection limit of 0.1 ng mL^(-1) for cortisol and 0.1 mM for lactate was established and a correlation between concentration and current was observed. A portable, handheld potentiostat assembled with Bluetooth communication and battery operation enables the developed system for point-of-care applications. A sandwich-like structure containing the sensing mechanisms as a prototype was designed to secure the biosensor to skin and use capillary action to draw sweat or other fluids toward the sensing mechanism. Overall, the immunosensor shows remarkable specificity, sensitivity as well as the noninvasive and point-of-care capabilities and allows the biosensor to be used as a versatile sensing platform in both developed and developing countries.

关键词： Graphene Immunosensor Electrochemical screen-printed electrodes Cortisol sensor

Disposable Amperometric Acetylcholinesterase Biosensor for the Detection of Organophosphorus Pesticides

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稀有金属材料与工程 2006年第A03期35卷 333-336页

作者： Xiaohao Wang Xiaowen Xu Fei Tang Zhaoying Zhou State Key Laboratory of Precision Measurement Technology and Instruments Tsinghua University Beijing 100084 China

A rapid,simple,disposable and inexpensive acetylcholinesterase (ACHE) amperometric biosensor for the detection of organophosphorus pesticides was developed by simple adsorption of the enzyme on screen-printed *** biosensor consisted of an Ag/AgCl reference electrode and a graphite working *** mixture of graphite and the 7,7,8,8-tetracyanoquinodimethane (TCNQ) was printed on *** detection of organophosphorus pesticides was done with acetylthiocholine chloride (ATCh) as *** biosensor was used to detect the inhibitory effect of organophosphorus pesticides on AChE *** 1μl of enzyme solution containing 0.1 U AChE and 1% bovine serum albumin (BSA) were simply dropped on the working electrode *** biosensor operated at a potential of 300 mV vs. Ag/AgCl in a pH 7.2 0.1 mol/L phosphate buffer and 0.1 mol/L *** obtained a calibration plot of the percentage inhibition versus the logarithm of parathion methyl concentration following an incubation time of 10 mix in parathion methyl solution. The lowest detectable amount of parathion methyl was 0.026 *** amperometric biosensor based on acetylcholinesterase was disposable and low cost (about 1 yuan RMB).

关键词： amperometric biosensor AChE screen-printed electrodes organophosphorus pesticides

The Influencing Factors of Disposable Acetylcholinesterase Biosensor for in Situ Detection of Organophosphorus Pesticide

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稀有金属材料与工程 2006年第A03期35卷 389-391页

作者： Xiaowen Xu Xiaohao Wang Fei Tang Zhaoying Zhou State Key Laboratory of Precision Measurement Technology and Instruments Tsinghua University Beijing 100084 China

An amperometric biosensor based on acetylcholinesterase (ACHE) is assembled by simple adsorption of the AChE on 7,7,8,8-tetracyanoquinodimethane (TCNQ) modified screen-printed *** biosensor is used to detect the inhibitory effect of organophosphorus pesticides on AChE *** detection of organophosphorus pesticides is done with acetylthiocboline chloride (ATCh) as *** order to obtain the optimized response to substrate,the influencing factors of the biosensor are investigated,including temperature,pH,incubation time,substrate concentration and AChE concentration. The measurements were performed after inhibition by immersing the enzyme electrode into the parathion methyl solution. Under the optimized conditions,that is,500 U/ml AChE concentration,pH 7.2,10 min incubation time,2 mmol/L substrate concentration and temperature of 37℃,from 5×10^(-8) mol/L to 5×10^(-5) mol/L is close to linear (regression equation: y(%)=124.055+15.7991gx,R^2=0.99644),which corresponds to 8.7%～56.1% AChE *** the optimized conditions, the lowest detectable amount of parathion methyl is 13×10^(-9).

关键词： biosensor AChE screen-printed electrodes influencing factors organophosphorus pesticides