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Inhibition of Platelet Aggregation and In Vitro Free Radical scavenging Activity of Dried Fruiting Bodies of Pleurotus eous

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Chinese Journal of Integrative Medicine 2015年第7期21卷 530-536页

作者： Suseem S.R Mary Saral Pharmaceutical Chemistry Division School of Advanced SciencesVIT University

Objective： To evaluate the ethyl acetate, methanol and aqueous extracts of dried fruiting bodies of Pleurotus eous for its anti-platelet activity on human volunteer＇s blood. And also to analyze the free radical scavenging property of the extracts of *** by using various in vitro models. Methods： Anti-platelet activity of dried fruiting bodies of *** was evaluated by in vitro model using blood platelets. Inhibition of platelet aggregation was monitored after pre-incubation of platelets with the crude extracts of mushroom ***. Antioxidant activities of extracts of *** were evaluated by different in vitro experiments, namely, 1, 1-diphenyl- 2-picryl hydrazyl （DPPH）, superoxide, hydroxyl radical and lipid peroxide radical models. Results： Crude extracts of mushroom *** inhibited platelet aggregation dose-dependently which was induced by adenosine diphosphate （ADP）. At a maximum concentration of 10 mg/mL, methanol extract effected 64.02% inhibition of lipid per-oxidation and 50.12% scavenging effect on superoxide anion radical. Aqueous extract of *** have shown 69.43% chelating ability on ferrous ions, 24.27% scavenging effect on hydroxyl radical and 49.57% scavenging effect on DPPH radical at 10 mg/mL. Increasing concentrations of the extract were found to cause progressively decreasing of the intensity of absorbance. Conclusions： Anti-platelet effects could be related in part to the polyphenolic compounds present in the extracts. Antioxidant activity results indicated the free radical scavenging property of the extracts of *** which might be due to the high content of phenolic compounds and flavonoids.

关键词： anti-platelet Pleurotus eous mushroom antioxidant scavenging effect chelating effect,reductive

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Study on scavenging and Disconnecting effects of Nitrosation Reaction by Extracts of Tetradium Ruticarpum and Glycyrrhizae

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Agricultural Science & Technology 2014年第6期15卷 970-976页

作者：张成王应江吴高箭魏玉娴董侯燕颜亨梅北京师范大学珠海分校工程技术学院生物工程系广东珠海519087

In order to investigate and compare the effects of Tetradium ruticarpum and Glycyrrhizae extracts in terms of scavenging sodium nitrite and inhibiting N-ni- troso compounds formation, the 1_9（3^3） orthogonal test was conducted to extract the active components. In addition, the capacities of scavenging sodium nitrite and of in- hibiting the N-nitroso compounds formation were measured to confirm the optimum condition of extracting. When the scavenging rate was considered as the main index, the maximum capacities of scavenging sodium nitrite by the extracts of Tetradi- urn ruticarpum and Glycyrrhizae were 0.836 5 and 0.558 0, respectively. Further- more, when the disconnection rate was considered as the main index, the maximum capacities of inhibiting the N-nitroso compounds formation by the extracts of Tetradi- urn ruticarpum and G/ycyrrhizae were 0.922 8 and 0.625 6, respectively. As a re- suit, the capacity of inhibiting nitrosation reaction by Tetradium ruticarpum was shown to be much stronger than G/ycyrrhizae, no matter the scavenging rate or the disconnection rate as the main index to be considered.

关键词： Tetradium ruticarpum and Glycyrrhizae extracts Disconnecting effect scavenging effect Sodium nitrite N-Nitro compounds

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