Inhibition of Platelet Aggregation and In Vitro Free Radical Scavenging Activity of Dried Fruiting Bodies of Pleurotus eous

作     者：Suseem S.R Mary Saral 

作者机构：Pharmaceutical Chemistry DivisionSchool of Advanced SciencesVIT University 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2015年第21卷第7期

页      面：530-536页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：VIT University management 

主　　题：anti-platelet Pleurotus eous mushroom antioxidant scavenging effect chelating effect,reductive 

摘      要：Objective： To evaluate the ethyl acetate, methanol and aqueous extracts of dried fruiting bodies of Pleurotus eous for its anti-platelet activity on human volunteer＇s blood. And also to analyze the free radical scavenging property of the extracts of *** by using various in vitro models. Methods： Anti-platelet activity of dried fruiting bodies of *** was evaluated by in vitro model using blood platelets. Inhibition of platelet aggregation was monitored after pre-incubation of platelets with the crude extracts of mushroom ***. Antioxidant activities of extracts of *** were evaluated by different in vitro experiments, namely, 1, 1-diphenyl- 2-picryl hydrazyl （DPPH）, superoxide, hydroxyl radical and lipid peroxide radical models. Results： Crude extracts of mushroom *** inhibited platelet aggregation dose-dependently which was induced by adenosine diphosphate （ADP）. At a maximum concentration of 10 mg/mL, methanol extract effected 64.02% inhibition of lipid per-oxidation and 50.12% scavenging effect on superoxide anion radical. Aqueous extract of *** have shown 69.43% chelating ability on ferrous ions, 24.27% scavenging effect on hydroxyl radical and 49.57% scavenging effect on DPPH radical at 10 mg/mL. Increasing concentrations of the extract were found to cause progressively decreasing of the intensity of absorbance. Conclusions： Anti-platelet effects could be related in part to the polyphenolic compounds present in the extracts. Antioxidant activity results indicated the free radical scavenging property of the extracts of *** which might be due to the high content of phenolic compounds and flavonoids.