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Effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic bacteria

Asian Pacific Journal of Tropical Medicine 2016年第2期9卷 115-120页

作者： Herastuti Sulistyani Mari Fujita Hiroshi Miyakawa Futoshi Nakazawa Department of Oral Microbiology School of Dentistry Health Sciences University of Hokkaido

Objective: To investigate the effect of the roselle calyx extract(RCE)(Hibiscus sabdariffa L.) on the in vitro viability and biofilm formation ability of oral pathogenic bacteria. Methods:RCE was prepared by soaking roselle calyx powder with ethyl alcohol for 24 h at room temperature. After centrifugation, the extract was lyophilized. Then, the extract was dissolved in phosphate-buffered saline, the p H was adjusted, and the extract was aseptically filtered. We used Streptococcus mutans, Streptococcus sanguinis, Lactobacillus casei, Actinomyces naeslundii, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia in this study. The antibacterial activity of the RCE was determined by treating the cells of these bacteria with the extract for 10 or 20 min at room temperature. The minimum inhibitory concentration(MIC) and minimum bactericidal concentration was determined using the micro dilution method, and the effect of the RCE on the ability to form biofilm was determined using a polystyrene micro plate assay. In addition, we used the WST-1 assay to determine the cytotoxicity of the RCE on HGF, Ca9-22 and KB cells. Results: The RCE had antibacterial activity against oral bacteria used in this study. In particular, most significant antibacterial activity was observed against Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis. The MIC and minimum bactericidal concentration were 7.2 mg/m L–28.8 mg/m L and 14.4 to >57.6 mg/m L. The RCE had an inhibitory effect on biofilm formation at the MIC and sub-MIC levels. In addition, the RCE had low cytotoxic effects on HGF, Ca9-22 and KB cells. Conclusions: Thus, our results indicate that the RCE may be used for preventing oral diseases.

关键词： Oral bacteria Oral biofilm Oral cells roselle calyx extract

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Study on Production Process of roselle Rice Wine

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Agricultural Biotechnology 2022年第5期11卷 116-120,129页

作者： Fangjun LIAO Sumei HUANG Huizhou Engineering Vocational College Huizhou 516023China

[Objectives]This study was conducted to more systematically explore the traditional brewing process of roselle rice wine. [Methods] With local high-quality white glutinous rice and sun-dried roselle as raw materials for brewing rice wine, on the premise of extensive research on traditional rice wine technology in China, the addition amount of roselle extract, the addition amount of distiller’s yeast, the variation range of after-fermentation temperature and the starting time of after-fermentation were investigated. [Results] The best traditional processing and brewing parameters were the ratio of glutinous rice and roselle extract(mass to volume ratio) at about 1∶1.55, the addition amount of distiller’s yeast of 0.55%, and the fermentation temperature at 30 ℃, and the starting time at 72 h. The sensory quality of the high-quality roselle rice wine that was produced under the best brewing conditions was about(87.7±2.45) points. [Conclusions] A number of hygiene inspection technical indexes in the product all met the current relevant national safety standards in China, providing another technical research direction for the development of a variety of new green healthy beverage projects.

关键词： roselle Sticky rice Rice wine Fermentation technology

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Effect of Organic and Inorganic Phosphorous on Growth of roselle(Hibiscus sabdariffa L.)

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Journal of Northeast Agricultural University(English Edition) 2016年第3期23卷 23-30页

作者： Hasnain Alam Muhammad Razaq Salahuddin Jaffar Khan Department of Bioinformatics and Biotechnology International Islamic University Department of Silviculture Northeast Forestry University Department of Horticulture Faculty of Crop Production Sciences University of Agriculture Peshawar

To investigate the performance of organic and inorganic phosphorous on growth of roselle （Hibiscus sabdariffa L.）, an experiment was carried out at Newly Developmental Farm （NDF）, Horticulture Section, the university of Agriculture Peshawar, Pakistan, during 2012. The experiment was laid out in a Randomized Complete Block Design （RCBD） with split plot arrangement and replicated three times. The chemical fertilizer Single Super Phosphate （SSP） was used as inorganic source of phosphorus, while Organic Phosphate （OP） from organic product produced by Niha corporation containing 20% organic mineralized P205, was used as the source of organic phosphorous. Both the sources of fertilizer were applied at the rate of 10, 20, 30 40 and 50 kg. hm2 and control. The statistical analysis of data revealed that both the sources and levels of phosphorus significantly affected most of the growth parameters of roselle except germination percentage and survival percentage. Plant height （112.09 era）, numbers of leave plant1 （177.87）, numbers of branch plant1 （4.08）, leaf areas （126.60 cm2）, days to flowering （142.83）, fresh flower weight （2.56 g）, fresh calyx weight （1.70 g）, dry calyx weight （0.43 g）, numbers of calyces （94.10）, numbers of root plant-1 （11.03）, root length （22.67 cm） and root weight （27.50 g） were observed in plants fertilized with organic source of phosphorous. Phosphorous levels significantly affected all of the parameters except germination percentage and survival percentage. The maximum plant height （124.39 cm）, numbers of branches （5.32）, numbers of leave plantl （204.89）, leaf areas （148.14 cm2）, fresh flower weight （3.32 g）, fresh calyx weight （2.04 g）, dry calyx weight （0.51 g）, numbers of calyces （105.30）, numbers of root plant-1 （12.78）, root length （24.50 cm） and root weight （29.94 g） were observed in plants fertilized with 40 kg. hm-2 phosphorous and the maximum numbers of days to flowering （148.17） were observed in the control plants. It was concluded from t

关键词： organic farming organic phosphorous roselle

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Comparative Studies on Egyptian and Libyan roselle Seeds as a Source of Lipid and Protein

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Food and Nutrition Sciences 2014年第22期5卷 2237-2245页

作者： Nady A. Elneairy Department of Food Science and Technology Faculty of Agriculture Fayoum University Al Fayoum Egypt

Proceeding from the fact that the seeds of roselle plant are full of nutritional constituents, however in Egypt and Libya, they are often discarded as a by-product, this study aims to evaluate the nutritional composition of roselle seeds grown in Egypt and Libya as a source of oil and protein besides making a comparison between whole chemical composition of roselle seeds grown in both countries. Ground of whole Egyptian and Libyan roselle seeds powder contained high amount of protein (31.02% ± 0.93% and 28.67% ± 0.45%), crude fat (21.6% ± 0.66% and 16.94% ± 0.86%) and total ash (6.89% ± 0.11% and 5.60% ± 0.10%), respectively. However, Egyptian seeds have moisture content, protein, crude fat and total ash higher than Libyan seeds. Crude oil from Egyptian seeds had high refractive index and iodine value in comparison with crude oil from Libyan seeds. There were no remarkable differences between both seeds in acidity percent, unsaponifiable matters percent and saponification value. Linoleic, oleic and palmitic acids were the major fatty acid constituents in Egyptian roselle seeds. Meanwhile linolenic, linoleic, oleic, stearic, palmitoleic and palmitic acids were the major fatty acid constituents in Libyan roselle seeds. Crude oil from Egyptian seeds had higher percent of unsaturated fatty acids than crude oil from Libyan seeds. Unsaponifiable matters constituents for extracted oil from Egyptian seeds were free from n-pentacosane (C25) and rich in n-hexacosane (C26). Oil from both seeds had the same content of Beta sito-sterol and stigma-sterol. Both seeds were rich in glutamic acid, aspartic acid, arginine and leucine. Libyan seeds were rich in essential amino acids in comparison with Egyptian seeds. Finally nutritional comparison of roselle seeds variation depends on the variety, location and environmental conditions during cultivation. roselle seeds are a good source for extraction of oil and protein. Protein from roselle seeds could be used as a supplement material

关键词： roselle Seed Proximate Analysis Oil Properties Fatty Acids Profile Unsaponifiable Matters Amino Acids Profile

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A Review of the Nutritional Health Effects of roselle (Hibiscus sabdariffa L.) Calyces

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Agricultural Biotechnology 2019年第5期8卷 21-25页

作者： Xuefang XIE Zhao LIU Xiaodong XIE Yongfa ZHANG Institute of Rubber Chinese Academy of Tropical Agricultural SciencesDanzhou 571737China Hainan Health School/Key Laboratory of Biology and Genetic Resources of Rubber Tree Ministry of Agriculture and Rural Affairs/State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical CropsDanzhou 571737China

roselle ( Hibiscus Sabdariffa L.) is a tropical and subtropical *** paper reviewed the distribution of roselle in China,and the varieties of roselle grown in *** main chemical components of roselle calyces were listed,and the nutritional health effects and the latest development and utilization of roselle calyx were summarized,in order to provide a reference for better promotion and cultivation of roselle.

关键词： roselle calyx Planting distribution Chemical composition Nutrition

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Herbal extracts counteract cisplatin-mediated cell death in rat testis

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Asian Journal of Andrology 2008年第2期10卷 291-297页

作者： Amr Amin Alaaeldin A. Hamza Amr Kambal Sayel Daoud Biology Department United Arab Emirates University AI-Ain 17551 United Arab Emirates Hematology Laboratory Twain Hospital At-Ain 15258 United Arab Emirates Histopathology Laboratory Twain Hospital At-Ain 15258 United Arab Emirates

Aim： To evaluate the protective effects of ginger （Gin） and roselle （Ros） against testicular damage and oxidative stress in a cisplatin （CIS）-induced rodent model. Their protective effects against CIS-induced apoptosis in testicular and epididymal sperms is also investigated. Methods： Ethanol extracts of Gin or Ros （1 g/***） were given orally to male albino rats for 26 days. This period began 21 days before a single CIS intraperitoneal injection （10 mg/kg body weight）. Results： Gin or Ros given orally significantly restored reproductive function. Both tested extracts notably reduced the CIS-induced reproductive toxicity, as evidenced by restoring the testis normal morphology. In Gin and Ros, the attenuation of CIS-induced damage was associated with less apoptotic cell death both in the testicular tissue and in the sperms. CIS-induced alterations of testicular lipid peroxidation were markedly improved by these plant extracts. Conclusion： The present results provide further insights into the mechanisms of protection against CIS-induced reproductive toxicity and confirm the essential anti-oxidant potential of both examined extracts. （Asian J Androl 2008 Mar; 10： 291-297）

关键词： cisplatin cell death toxicity flow cytometry ginger roselle

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Potential of Thai Bast Fibers for Injection Molded PLA Composites

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Journal of Renewable Materials 2023年第5期11卷 2279-2300页

作者： Nina Graupner Thiprada Poonsawat Koranat Narkpiban Jörg Müssig The Biological Materials Group Department of BiomimeticsHSB-City University of Applied SciencesBremenD-28199Germany Department of Botany Faculty of Liberal Arts and ScienceKasetsart UniversityKamphaeng SaenNakhon Pathom73140Thailand Bioproduct Science Department of ScienceFaculty of Liberal Art and ScienceKasetsart UniversityKamphaeng SaenNakhon Pathom73140Thailand Department of Science and Mathematics Faculty of Science and TechnologyRajamangala University of Technology Tawan-OkChonburi20110Thailand

Thailand has a huge variability of bast fiber plants,some of which have been little researched regarding their applicability in *** fiber(bundle)s from different species were investigated and incorporated into a polylactide(PLA)matrix by injection *** and kenaf were used as well-studied fibers,while roselle,Fryxell and paper mulberry are less extensively *** strength,tensile modulus and interfacial shear strength(IFSS)of single fiber(bundle)s were highest for hemp,followed by kenaf,roselle,Fryxell and paper *** the lower tensile strength and IFSS of paper mulberry,the highest tensile strength was achieved for the paper mulberry/PLA composite followed by hemp/*** electron microscope(SEM)analyses showed that the single cells in paper mulberry fiber bundles,in contrast to the other fiber types investigated,were only partially bonded to each other,which explains the lower strength of the fiber *** higher aspect ratio of fiber(bundle)s of paper mulberry in the PLA composite can explain the good composite *** from hemp,paper mulberry shows the best reinforcing effect in the PLA matrix and offers interesting potential for composite *** to neat PLA,the tensile strength could be increased by 24%and the tensile modulus by 54%.

关键词： Mechanical properties interfacial shear strength aspect ratio roselle Fryxell paper mulberry

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