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Genome-wide analysis of expression quantitative trait loci(eQTLs)reveals the regulatory architecture of gene expression variation in the storage roots of sweet potato

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Horticulture Research 2020年第1期7卷 1713-1724页

作者： Lei Zhang Yicheng Yu Tianye Shi Meng Kou Jian Sun Tao Xu Qiang Li Shaoyuan Wu Qinghe Cao Wenqian Hou Zongyun Li Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics School of Life SciencesJiangsu Normal UniversityXuzhou 221116 Jiangsu ProvincePeople’s Republic of China Xuzhou Academy of Agricultural Sciences/Sweet Potato Research Institute CAASXuzhou 221121 Jiangsu ProvincePeople’s Republic of China

Dissecting the genetic regulation of gene expression is critical for understanding phenotypic variation and species ***,our understanding of the transcriptional variability in sweet potato remains ***,we analyzed two publicly available datasets to explore the landscape of transcriptomic variations and its genetic basis in the storage roots of sweet *** comprehensive analysis identified a total of 724,438 high-confidence single nucleotide polymorphisms(SNPs)and 26,026 expressed *** quantitative trait locus(eQTL)analysis revealed 4408 eQTLs regulating the expression of 3646 genes,including 2261 local eQTLs and 2147 distant *** distant eQTL hotspots were found with target genes significantly enriched in specific functional *** combining the information from regulatory network analyses,eQTLs and association mapping,we found that IbMYB1-2 acts as a master regulator and is the major gene responsible for the activation of anthocyanin biosynthesis in the storage roots of sweet *** study provides the first insight into the genetic architecture of genome-wide expression variation in sweet potato and can be used to investigate the potential effects of genetic variants on key agronomic traits in sweet potato.

关键词： roots analysis sweet

PURIFICATION OF Cd-BINDING PROTEIN FROM MAIZE roots BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

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Science China Chemistry 1991年第12期34卷 1461-1468页

作者：何笃修罗建沅全胜 Department of Biology Peking University Beijing 100871 PRC

An efficient procedure of purification of Cd-binding protein in roots of maize has been established. Young seedlings of maize were exposed to a medium containing CdCl2 to induce the production of Cd-binding protein in their roots. The protein was purified after heat treatment by ion-exchange chromatography and reverse-phase HPLC. The resulting protein was identified as a purified product by N-terminal amino acid with the dansyl method. Its molecular weight was 3200 dalton, the cysteine content was 29.5%, about 3 Cd atoms were bound to one molecule of the protein and the Cd : cystine ratio was 1 : 2.3. According to its character, this protein could be a kind of plant metallothionein-like protein.

关键词： maize roots Cd binding protein heat treatment HPLC amino acid composition.

INFECTION OF Ri T-DNA-TRANSFORMED roots BY Plasmodiophora brassicae IN Brassica CROPS

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Science China Chemistry 1991年第12期34卷 1444-1451页

作者：何玉科 Department of Horticulture Northwestern Agricultural University Yangling Shaanxi 712100 PRC

Many Ri T-DNA-transformed roots were incited on seedling hypocotyl segments of cabbage, cauliflower and oilseed rape following genetic transformation with Agribacterium rhi-zogencs. The transformed roots were in vitro inoculated with the resting spores of Plasmodi-ophora brassicae on solid medium, in liquid medium and semi-solid medium *** solid medium, the resting spores penetrated root hair cells, but the root cortex was not invaded so that no symptom of clubroot galls was developed. In liquid medium, the root cortex was invaded, giving rise to an abnormal division of root cortical tissues and gall formation. In semi-solid medium, the resting spores penetrated the cells of the cut surfaces of root segments and caused the cut surfaces to swell.

关键词： Agrobacterium rhizogenes transformed roots Plasmodiophora brassicae plasmodia.

The MdABI5 transcription factor interacts with the MdNRT1.5/MdNPF7.3 promoter to fine-tune nitrate transport from roots to shoots in apple

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Horticulture Research 2021年第1期8卷 3131-3143页

作者： Ya-Jing Liu Ning Gao Qi-Jun Ma Jiu-Cheng Zhang Xun Wang Jing Lu Yu-Jin Hao Xiao-Fei Wang Chun-Xiang You State Key Laboratory of Crop Biology Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China

Nitrate is a major nitrogen resource for plant growth and development and acts as both a crucial nutrient and a signaling molecule for plants;hence,understanding nitrate signaling is important for crop *** acid(ABA)has been demonstrated to be involved in nitrate signaling,but the underlying mechanism is largely unknown in *** this study,we found that exogenous ABA inhibited the transport of nitrate from roots to shoots in apple,and the transcription of the nitrate transporter MdNRT1.5/MdNPF7.3 was noticeably reduced at the transcriptional level by ABA,which inhibited the transport of nitrate from roots to ***,it was found that the ABA-responsive transcription factor MdABI5 bound directly to the ABRE recognition site of the MdNRT1.5 promoter and suppressed its *** of MdABI5 inhibited ABA-mediated transport of nitrate from roots to ***,these results demonstrate that MdABI5 regulates the transport of nitrate from roots to shoots partially by mediating the expression of MdNRT1.5,illuminating the molecular mechanism by which ABA regulates nitrate transport in apple.

关键词： ABI5 nitrate roots

Overexpression of phosphatidylserine synthase IbPSS1 affords cellular Na+homeostasis and salt tolerance by activating plasma membrane Na^(+)/H^(+)antiport activity in sweet potato roots

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Horticulture Research 2020年第1期7卷 1235-1246页

作者： Yicheng Yu Ying Xuan Xiaofeng Bian Lei Zhang Zhiyuan Pan Meng Kou Qinghe Cao Zhonghou Tang Qiang Li Daifu Ma Zongyun Li Jian Sun Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics School of Life SciencesJiangsu Normal University221116 XuzhouJiangsuChina Institute of Food Crops Provincial Key Laboratory of AgrobiologyJiangsu Academy of Agricultural Sciences210014 NanjingChina Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District 221131 XuzhouJiangsu

Phosphatidylserine synthase(PSS)-mediated phosphatidylserine(PS)synthesis is crucial for plant ***,little is known about the contribution of PSS to Na^(+)homeostasis regulation and salt tolerance in ***,we cloned the IbPSS1 gene,which encodes an ortholog of Arabidopsis AtPSS1,from sweet potato(Ipomoea batatas(L.)Lam.).The transient expression of IbPSS1 in Nicotiana benthamiana leaves increased PS *** then established an efficient Agrobacterium rhizogenes-mediated in vivo root transgenic system for sweet *** of IbPSS1 through this system markedly decreased cellular Na^(+)accumulation in salinized transgenic roots(TRs)compared with adventitious *** overexpression of IbPSS1 enhanced salt-induced Na^(+)/H^(+)antiport activity and increased plasma membrane(PM)Ca^(2+)-permeable channel sensitivity to NaCl and H2O2 in the *** confirmed the important role of IbPSS1 in improving salt tolerance in transgenic sweet potato lines obtained from an Agrobacterium tumefaciens-mediated transformation ***,compared with the wild-type(WT)plants,the transgenic lines presented decreased Na^(+)accumulation,enhanced Na^(+)exclusion,and increased PM Ca^(2+)-permeable channel sensitivity to NaCl and H2O2 in the *** application of lysophosphatidylserine triggered similar shifts in Na^(+)accumulation and Na^(+)and Ca^(2+)fluxes in the salinized roots of ***,this study provides an efficient and reliable transgenic method for functional genomic studies of sweet *** results revealed that IbPSS1 contributes to the salt tolerance of sweet potato by enabling Na^(+)homeostasis and Na^(+)exclusion in the roots,and the latter process is possibly controlled by PS reinforcing Ca^(2+)signaling in the roots.

关键词： roots homeostasis potato

Smallanthus sonchifolius roots ameliorate non-alcoholic fatty liver disease by reducing redox imbalance and hepatocyte damage in rats fed with a high fructose diet

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Asian Pacific Journal of Tropical Biomedicine 2019年第9期9卷 365-372页

作者： Mariano Nicolás Alemán Sara Serafina Sánchez Stella Maris Honoré Instituto Superior de Investigaciones Biológicas (INSIBIO) Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Tucumán (CONICET-UNT)

Objective: To evaluate the potential of Smallanthus sonchifolius(S. sonchifolius) roots in ameliorating hepatic damage of rats fed with a high fructose ***: The effect of S. sonchifolius roots on energy intake, body weight, fat and liver mass was determined in male rats fed with a high-fructose diet. Plasma glucose, triglycerides, total cholesterol, lipoproteins and aspartate aminotransferase and alanine aminotransferase were analyzed. Histological changes of the livers were evaluated by electronic microscopy and apoptosis was examined using the TUNEL method. The levels of malondialdehyde, reducedglutathione and antioxidant enzymes(catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase) activities were also ***: S. sonchifolius roots significantly decreased energy intake, body weight, fat and liver mass(P roots ameliorated liver steatosis and mitochondrial morphology, avoiding cellular apoptosis and normalizing transaminase activity in the liver of rats fed with high fructose. Enzymatic assays revealed that S. sonchifolius roots had a modulatory effect on the oxidative stress induced by fructose-feeding by reducing lipid peroxidation(P roots can ameliorate non-alcoholic fatty liver disease by improving oxidative stress and liver injury.

关键词： Smallanthus sonchifolius roots Non-alcoholic fatty liver disease Oxidative stress Apoptosis Liver

Comparative transcriptome analysis reveals synergistic and disparate defense pathways in the leaves and roots of trifoliate orange(Poncirus trifoliata)autotetraploids with enhanced salt tolerance

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Horticulture Research 2020年第1期7卷 1689-1702页

作者： Tonglu Wei Yue Wang Ji-Hong Liu Key Laboratory of Horticultural Plant Biology(MOE) College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China

Polyploid plants often exhibit enhanced stress tolerance relative to their diploid counterparts,but the physiological and molecular mechanisms of this enhanced stress tolerance remain largely *** this study,we showed that autotetraploid trifoliate orange(Poncirus trifoliata(L.)Raf.)exhibited enhanced salt tolerance in comparison with diploid *** transcriptome profiling of diploid and tetraploid plants with or without salt stress by RNAseq revealed that the autotetraploids displayed specific enrichment of differentially expressed ***,the leaves and roots of tetraploids exhibited different expression patterns of a variety of upregulated *** related to plant hormone signal transduction were enriched in tetraploid leaves,whereas those associated with starch and sucrose metabolism and proline biosynthesis were enriched in *** addition,genes encoding different antioxidant enzymes were upregulated in the leaves(POD)and roots(APX)of tetraploids under salt ***,the tetraploids accumulated higher levels of soluble sugars and proline but less ROS under salt stress compared to the ***,several genes encoding transcription factors were induced specifically or to higher levels in the tetraploids under salt ***,this study demonstrates that the activation of various multifaceted defense systems in leaves and roots contributes to the enhanced salt tolerance of autotetraploids.

关键词： RNAseq roots orange

PRELIMINARY STUDY ON MECHANISM OF PLANT roots TO INCREASE SOIL ANTISCOURIBILITY ON THE LOESS PLATEAU

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Science China Chemistry 1992年第9期35卷 1085-1092页

作者：李勇徐晓琴朱显谟 Institute of Geochemistry Academia Sinica Guiyang 550002 PRC Northwest Institute of Soil and Water Conservation Academia Sinica and Ministry of Water Conservancy Yangling 712100 PRC

The effects of the root system of trees, shrubs and grasses on the hydraulic andphysical properties of soil and their interrelation with increasing soil anti--scouribility byroots are quantitatively demonstrated in this paper for the first time. Based on the analysisof the leading factors of roots to increase the soil anti--scouribility, a mathematical modelon increasing soil anti-scouribility by roots is established. The value in application of themodel is to explain the mechanism of roots to increase the soil anti-scouribility and toevaluate the increasing effects of soil anti-scouribility by roots in different layers of soilThe calculated results of the values in soil anti--scouribility intensified by roots at variousintensities of rainfall and slopes of the model have higher precision, all of the meanmaximum deviations between the estimated and observed values being less than 1.417 s/g.

关键词： plant roots soil anti-scouribility hydraulic properties of soil mathematical model the Loess Plateau

Digging the metabolic roots of NASH up

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Life Metabolism 2022年第3期1卷 203-204页

作者： Enrica Baldelli Amedeo Lonardo Department of Biomedical Metabolic and Neural SciencesUniversity of Modena and Reggio EmiliaModenaItaly Azienda Ospedaliero-Universitaria di Modena—Department of Internal Medicine—Ospedale Civile di Baggiovara ModenaItaly

“Maybe you are searching among the branches for what only appears in the roots”—*** the paradigm of disease heterogeneity,nonalcoholic fatty liver disease(NAFLD)has a remarkably variable natural history in the individual patient which legitimates personalized medicine *** some individuals follow a benign indolent course while others progress from simple steatosis to ongoing liver fibrosis,cirrhosis,and hepatocellular carcinoma(HCC)remains a fundamental(though unanswered)clinical and research question.

关键词： roots liver NAFLD