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Enzymatic reaction of ethanol and oleic acid by lipase and lignin peroxidase in rhamnolipid(RL) reversed micelles

Journal of Central South University 2015年第8期22卷 2936-2944页

作者：包珊袁兴中彭馨曾光明武海鹏王侯刘欢马玉洁崔凯龙吴秀莲 College of Environmental Science and Engineering Hunan University Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education (Hunan University)

An environment friendly bio-surfactant of rhamnolipid(RL) was used as a solvent. The enzymatic reaction of oleic acid catalyzed by lipase and lignin peroxidase(lip) was evaluated. The optimum conditions of enzymatic reaction catalyzed by lipase(lip) were water to amphiphile molar ratio of 30(20), RL of 60(60) critical micelle concentration(CMC), pH of 7.0(3.0) and temperature of 40(30) °C, respectively. The change of enzyme conformation indicates that, for catalytic of lipase, water content is the most important factor of the enzymatic reaction of oleic acid, and p H for lip. With individual optimum conditions, the enzymatic efficiency of oleic acid catalyzed by lipase is higher than that by lip. In the presence of ethanol, the enzymatic reaction of oleic acid catalyzed by lipase suits Ping-Pong Bi-Bi mechanism. As an alternative to chemical reversed micelles, the RL reversed micelles are promising methods to enzymatic reaction of oleic acid.

关键词： lipase lip enzymatic reaction rhamnolipid reversed micelles oleic acid

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Selective Affinity Separation of Yeast Alcohol Dehydrogenase by Reverse micelles with Unbound Triazine Dye

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Chinese Journal of Chemical Engineering 2001年第3期9卷 314-318页

作者：张天喜刘会洲陈家镛中科院化工冶金所北京

The reversed micelles were formed with cationic cetyltrimethylammonium bromide (CTAB) as surfactant and n-hexanol as cosolvent in the CTAB (50mmol.L-1)/hexanol (15% by volume)/hexane system. Cibacron Blue 3GA (CB) as an affinity ligand in the aqueous phase was directly introduced to the reversed micelles with electrostatic interaction between anionic CB and cationic surfactant. High molecular weight (Mr) protein, yeast alcohol dehydrogenase (YADH, Mr = 141000) from baker's yeast, has been purified using the affinity reversed micelles by the phase transfer method. Various parameters, such as CB concentration, pH and ionic strength, on YADH forward and backward transfer were studied. YADH can be transferred into and out from the reversed micelles under mild conditions (only by regulation of solution pH and salt concentration) with the successful recovery of most YADH activity. Both forward and backward extractions occurred when the aqueous phase pH＞pI with electrostatic attraction between YADH and CTAB. The recovery of YADH activity and purification factor have been improved with addition of a small amount of affinity CB. The recovery of YADH activity obtained was ～99% and the purification factor was about 4.0-fold after one cycle of full forward and backward extraction. The low ionic strength in the initial aqueous phase might be responsible for the YADH transfer into the reversed micellar phase.

关键词： reversed micelles yeast alcohol dehydrogenase protein purification affinity technology cetyltrimethy- lammonium bromide

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Review on aggregation of acid extractants in solvent extraction of metal ions:remark on the general model

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Progress in Natural Science:Materials International 2003年第1期 3-14页

作者： ZHOU Naifu and WU Jinguang (Department of Chemistry, Peking University, Beijing 100871, China Charles S. Roberts Laboratory, Gilmer Tumham Building, Auburn 36830, USA) Department of Chemistry，Peking University，Beijing 100871，China Charles S. Roberts Laboratory，Gilmer Tumham Building，Auburn 36830，USA Department of Chemistry，Peking University，Beijing 100871，China

Abstract The aggregation behavior of various acid extractants in the solvent extraction systems of metal ions is re-examined andexplained according to knowledge obtained in recent work. The conclusions are as follows. (1) Complexes formed by the extractants andmetal ions can form reversed micelles in organic diluents, depending on the microstructures of the complexes. The dimers of the acid ex-tractant cannot percolate to the metal-extractant aggregates, and the acid-salt complexes are always formed in the aggregates. The reversedmicelles or the W/O microemulsions formed by different species cannot be associated with each other to form a unified aggregate. (2) Insolvent extraction system, hydration of the extractants and metal ions can be considered as the driving force of forming reversed micelles.(3) Information of the first approach to the insight of the bicontinuous microemulsion of NaDEHP shows that various components in theaqueous phase behave confined and very similar to the typical AOT/n-heptane

关键词： liquid/liquid extraction complex aggregate reversed micelles W/O microemulsions.

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