Enzymatic reaction of ethanol and oleic acid by lipase and lignin peroxidase in rhamnolipid(RL) reversed micelles

作     者：包珊 袁兴中 彭馨 曾光明 武海鹏 王侯 刘欢 马玉洁 崔凯龙 吴秀莲 

作者机构：College of Environmental Science and Engineering Hunan University Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education (Hunan University) 

出 版 物：《Journal of Central South University》 (中南大学学报（英文版）)

年 卷 期：2015年第22卷第8期

页      面：2936-2944页

核心收录：

学科分类：0820[工学-石油与天然气工程] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 082002[工学-油气田开发工程] 

基　　金：Projects(50978087 51009063 50978088)supported by the National Natural Science Foundation of China 

主　　题：lipase lip enzymatic reaction rhamnolipid reversed micelles oleic acid 

摘      要：An environment friendly bio-surfactant of rhamnolipid(RL) was used as a solvent. The enzymatic reaction of oleic acid catalyzed by lipase and lignin peroxidase(lip) was evaluated. The optimum conditions of enzymatic reaction catalyzed by lipase(lip) were water to amphiphile molar ratio of 30(20), RL of 60(60) critical micelle concentration(CMC), pH of 7.0(3.0) and temperature of 40(30) °C, respectively. The change of enzyme conformation indicates that, for catalytic of lipase, water content is the most important factor of the enzymatic reaction of oleic acid, and p H for lip. With individual optimum conditions, the enzymatic efficiency of oleic acid catalyzed by lipase is higher than that by lip. In the presence of ethanol, the enzymatic reaction of oleic acid catalyzed by lipase suits Ping-Pong Bi-Bi mechanism. As an alternative to chemical reversed micelles, the RL reversed micelles are promising methods to enzymatic reaction of oleic acid.