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Clinical application of real time-polymerase chain reaction in determining cytomegalovirus viral DNA load in renal transplant recipients

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Chinese Medical Journal 2012年第19期125卷 3575-3577页

作者： ZHANG Chuan-bao LAI Hui-ying XU Hong-tao WANG Da-guang XIAO Fei National Center for Clinical Laboratories Beijing Institute of Geriatrics Beijing Hospital Ministry of Health Beijing 100730 China Department of Laboratory Medicine Beijing Institute of Geriatrics Beijing Hospital Ministry of Health Beijing 100730 China Key Laboratory of Geriatrics Beijing Institute of Geriatrics Beijing Hospital Ministry of Health Beiiing 100730 China

Background Cytomegalovirus （CMV） remains a significant clinical problem among immunosuppressed renal transplant patients. Quantitative PCR assays have become the most common methods in the determination of CMV infections in transplant patients. This study was to determine the relationship between CMV infection and the acute rejection of the transplanted kidney. Methods Plasma samples from 77 renal transplant patients that were pre-transplant negative for CMV infection were tested using reaD-time quantitative PCR and CMV gene-specific primers. The detected viral loads were retrospectively compared with the acute rejection rate and the chronic or mild rejection rates of the renal transplant. Results CMV-DNA was detected in 29 of 77 recipients, yielding a positive rate of detection of 37.7% for this procedure. Twelve of the 21 recipients （57.1%） who suffered acute rejection had positive CMV-DNA. Among the 56 recipients suffered from chronic or mild rejection, 17 （30.4%） had positive CMV-DNA plasma. Moreover, of the 29 recipients who had detectable CMV-DNA after transplant, 12 （41.4%） suffered from acute rejection; of the 48 recipients with undetectable CMV-DNA, only nine （18.8%） developed acute rejection. Post-transplant patients with acute rejection had a higher rate （57.1% vs. 30.4%, P=0.03） of post-transplant CMV infection than those with chronic or mild rejection. Conclusion CMV infection is a risk factor of acute renal transplant rejection and CMV infection should be prevented and treated in renal transplant recipients.

关键词： real time-polymerase chain reaction renal transplant cytomegalovirus infection rejection

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Survivin mRNA expression in urine as a biomarker for patients with transitional cell carcinoma of bladder

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Chinese Medical Journal 2006年第13期119卷 1118-1120页

作者： HOU Jian-quan HE Jun WEN Duan-gai CHEN Zi-xing ZENG Jian Department of Urology Jiangsu Institute of Hematology First Affiliated Hospital of Soochow University Suzhou 215006 China

Transitional cell carcinoma （TCC） of bladder is the most common malignant tumor in uropoiesis system. Up to date, there is still lack of an ideal marker for the diagnosis of TCC except CT and MRI imaging and cystoscopy. Cystoscopy is an invasive examination, which increases the possibility of urinary tract infection. Urine cytology has low sensitivity （21%--40%） in diagnosis of bladder cancer, especially for those with medium or high differentiation. The specificity is often affected by factors such as specimen collection, urinary tract infection, etc. Detecting the expression of survivin mRNA in urine by real time-PCR is simple in specimen collection and is sensitive and relatively specific, which provides a simple and noninvasive diagnostic method for TCC. Moreover it allows comparing the gene expression levels at different stages and grades of TCC, which can help define malignancy degree of TCC.

关键词： transitional cell carcinoma of bladder survivin real time-polymerase chain reaction

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