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Protective effects of estrogens and caloric restriction during aging on various rat testis parameters

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Asian Journal of Andrology 2008年第6期10卷 837-845页

作者： Khaled Hamden Dorothee Silandre Christelle Delalande Abdelfattah E1Feki Serge Carreau Department of Animal Ecophysiology Faculty of Sciences University of Sfax PB 802 Sfax 3018 Tunisia USC 2006 INRA- EA 2608 Biochemistry Caen-14032 France

Aim： To investigate the effects of 17β-estradiol （E2）, Peganum harmala extract （PHE） and caloric restriction （CR） on various testis parameters during aging. Methods： Twelve-month-old male rats were treated for 6 months with either E2 or PHE, or submitted to CR （40%）. Results： Our results show that estrogens and CR are able to protect the male gonad by preventing the decrease of testosterone and E2 levels as well as the decrease of aromatase and estrogen receptor gene expressions. Indeed, E2, PHE and CR treatments induced an increase in the superoxide dismutase activities and decreased the activity of testicular enzymes： gamma-glutamyl transferase, alkaline phosphatase, lactate deshydrogenase as well as the aspartate and lactate transaminases in aged animals. In addition, the testicular catalase and gluthatione peroxidase activities were enhanced in E2, PHE and CR-treated rats compared to untreated animals at 18 months of age. Moreover, the positive effects of estradiol, PHE and CR were further supported by a lower level of lipid peroxidation. Recovery of spermatogenesis was recorded in treated rats. Conclusion： Besides a low caloric diet which is beneficial for spermatogenesis, a protective antioxydant role of estrogens is suggested. Estrogens delay testicular cell damage, which leads to functional senescence and, therefore, estrogens are helpful in protecting the reproductive functions from the adverse effects exerted by reactive oxygen species （ROS） produced in large quanti- ties in the aged testis.

关键词： aging (phyto)estrogens 17β-estradiol Peganum harmala caloric restriction rat testis antioxidant enzymes

Identification of γ-aminobutyric acid transporter (GAT1)on the rat sperm

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Cell Research 2000年第1期10卷 51-58页

作者： HU JING HUA XIAO BING HE YUAN CHANG YAN (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031) ShanghaiInstituteofCellBiology ChineseAcademyofSciences.

Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.

关键词： Gat1 rat testis sperm expression.

Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56

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Cell Research 2003年第2期13卷 121-129页

作者： SS KOIDE The Population Council Center for Biomedical Research 1230 York Avenue New York New York 100021 USA

Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig. namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of 743 bp and 938 bp were amplified. The PCR products show very high homology to mouse sp56. However, the 743 bp product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743 bp product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that a -2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr -42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. rat sp56 was localized on rat sperm head by the indirect immunofluorescence method. rat sp56 immunoreactivi

关键词： ZP3 receptor sp56 rat testis sperm cloning.

Role of LM23 in cell proliferation and apoptosis and its expression during the testis development

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Asian Journal of Andrology 2013年第4期15卷 539-544,I0010页

作者： Qing Liu Ya-Juan Song Li-Jun Meng Fen Hu Li-Xia Gou Chang-Hong Jia Hong-Mei Tang Wei Jie Wang Mi Li Xiu-Jun Zhang Meng-Chun Jia College of Life Sciences Hebei United University Tangshan 063000 China Department of Clinical Laboratory Handan Central Hospital Handan 056002 China Department of Environment & Chemical Engineering Tangshan College Tangshan 063000 China and Department of Reproductive Endocrinology National Research Institute for Family Planning Beijing 100081 China

LM23, a gene expressed specifically in the testis in a stage-specific manner, has a diverse range of functions that are important in both the life and death of spermatogenic cells. The aim of this study was to further investigate the expression of LM23 in the developing rat testis and the biological function of LM23 in proliferation and antiapoptosis in vitro. Semiquantitative reverse transcription （RT）-PCR and real-time PCR were used to examine the expression of LM23 in testis at different developmental stages. The results suggested that LM23 mRNA levels in the testis increased progressively after birth. The role of LM23 in proliferation was analyzed with cell counting kit-8 （CCK8）, colony-forming efficiency （CFE） and flow cytometry assays. The results indicated that ectopic expression of LM23 in 293T cells significantly promoted cell proliferation by increasing cell numbers in S phase. Several methods were used, including CCK8, annexin V and propidium iodide staining and western blotting, to determine the role of LM23 in apoptosis. The results showed that LM23 played a protective role in H202-induced apoptosis of 293T cells, mediated at least in part through the Akt/PI3K signal pathway. Taken together, these results provide new insights into the role of LM23 in the development of the testes andspermatogenesis.

关键词： apoptosis LM23 proliferation rat testis

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Asian Journal of Andrology 2008年第2期10卷 177-187页

作者： Khaled Hamden Dorothee Silandre Christelle Delalande Abdefattah El Feki Serge Carreau Animal Ecophysiology Faculty of Sciences Sfax-3018 Tunisia USC 20061NRA- EA 2608 Biochemistry Caen-14032 France

Aim： To examine the effects on rat aging of caloric restriction （CR1） and undernutrition （CR2） on the body and on testicular weights, on two enzymatic antioxidants （superoxide dismutase and catalase）, on lipid peroxidation and on the expression of testicular aromatase and estrogen receptors （ER）. Methods： CR was initiated in 1-month-old rats and carried on until the age of 18 months. Results： In control and CR2 rats an age-related decrease of the aromatase and of ER （α and β） gene expression was observed; in parallel a diminution of testicular weights, and of the total number and motility of epididymal spermatozo was recorded. In addition, aging in control and CR2 rats was accompartied by a significant decrease in testicular superoxide dismutase, catalase activities, and an increase in lipid peroxidation level （thiobarbituric acid reactive substance）, associated with alterations of spermatogenesis. Conversely, caloric restriction-treatment exerted a protective effect and all the parameters were less affected by aging. Conclusion： These results indicate that during aging, a low caloric diet （not undernutrition） is beneficial for spermatogenesis and likely improves the protection of the cells via an increase of the cellular antioxidant defense system in which aromatase/ ER could play a role. （Asian J Andro12008 Mar; 10： 177-187）

关键词： aging low caloric diet rat testis aromatase estrogen receptors antioxidants

Further Analysis of El Tor Vibrio Cholerae Strains 1d:Comparison of Two Typing Methods

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The Journal of Biomedical Research 1995年第1期19卷 19-21页

作者：蒋小皖陈树昶蒋小陵

The lactate dehydrogenase isoenzyme C4 ofrat testicle was purified with a yield of 1 2.3% anda raise of specific activity to *** purified product was PAGE homogeneous and migrated afterLDH3 during electrophoresis.

关键词： rat testis LDH-C4 purification

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Isolation　and　Purification　of　rat　Testicular　Lactate　Dehydrogenase　C_4

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The Journal of Biomedical Research 1994年第1期18卷 18-18页

作者： Tan Weimin, et al.(ACTA ACADEMIAE MEDICINAE NANJING, 1994, 14 (2):156-157)

关键词： rat testis LDH-C4 purification

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