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Production and Optimization of pseudomonas fluorescensBiomass and Metabolites for Biocontrol of Strawberry Grey Mould

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American Journal of Plant Sciences 2012年第7期3卷 836-845页

作者： Wafaa M. Haggag Mostafa Abo El Soud Biotechnology and Genetic Engineering Unit National Research Center Dokki Cairo Egypt. Department of Plant Pathology National Research Centre Dokki Cairo Egypt

pseudomonas species have been widely studied as biological agents (BCAs) and it is alternative to the application of chemical fungicides. Our objective was to optimize nutritional and environmental conditions of the isolated pseudomonas fluorescens fp-5 for biomass and metabolites production and to evaluate itsagainst the grey mould disease caused by Botrytis cinerea on strawberry plants under field conditions. pseudomonas fluorescens, showed antagonistic properties, in vitro, against thepathogen Botrytiscinerea. Effect of the separated secondary metabolites on the fungal growth by broth dilution technique and antifungal activity by agar well diffusion technique was studied. Response surface methodology was used to investigate the effects of four fermentation parameters (pH, incubation time, carbon and nitrogen concentrations) on biomass and bioactivemetabolites [antibiotic phenazin and siderophore] production. Glycerol was found to be the best carbon source for improved biomass and metabolites production. Meanwhile, peptone and yeast extract were found to be the best nitrogen source. Analysis of each formulation revealed that glycerol oil at 0.01% the best oil used for protect P. fluorescens for 3 months Under natural condition, P. fluorescens formulation was effective in reducing B. cinerea disease in strawberry leaves and fruits. Pre-harvest treatment protected fruits from Botrytis post-harvest disease in comparing of fungicide. In addition, the obtained results showed that bacterial treatment significantly increased thegrowth parameters as well as dry weights and yield.

关键词： Botrytis Bunch Rot pseudomonas fluorescens Strawberry Blight

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Biofilm Development, Plant Growth Promoting Traits and Rhizosphere Colonization by pseudomonas entomophilaFAP1: A Promising PGPR

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Advances in Microbiology 2018年第3期8卷 235-251页

作者： Firoz Ahmad Ansari Iqbal Ahmad Biofilm Research Lab. Department of Agricultural Microbiology Faculty of Agricultural Sciences Aligarh Muslim University Aligarh India

Among the diverse soil bacteria, plant growth promoting rhizobacteria (PGPR) mark an important role in enhancing plant growth through a range of beneficial functions. This is mainly achieved by effective rhizosphere colonization by PGPR. Biofilm development by PGPR is considered as a survival strategy over the planktonic mode of growth under stress and natural conditions. Since the performance of microbial inoculants under field conditions is not always consistent due to various biotic and abiotic factors affecting survival, colonization and functions. Therefore, the rhizobacteria with efficient colonization ability and exhibiting multiple PGP traits are expected to perform better. We hypothesized that the biofilm forming ability of PGPR on plant root will be an added advantage to rhizosphere colonization. Therefore, we have selected a promising isolate of PGPR through random screening programme from rhizoplane of wheat (Triticum aestivum). The selection was based on biofilm development ability, multifarious PGP activities (production of indole acetic acid, sidero-phore, phosphate solubilization, hydrogen cyanide, ammonia production and biocontrol activity) and tolerance to salinity and heavy metals. The selected isolate was identified by 16 s rRNA partial gene sequencing as pseudomonas entomophila-FAP1. The strain FAP1 formed strong biofilm in microtitre plate, glass surface as well as on the roots of wheat seedlings. Biofilm forming capacity of the FAP1 was characterized by scanning electron microscopy and confocal laser scanning microscopy. FAP1 exhibited biofilm-related traits such as the production of exopolysaccharides, EPS (1501.33 ± 1.08 μg ml-1), alginate (212.81 ± 1.09 μg ml-1), swarming motility (22 ± 1.36 mm), swimming motility (31 ± 2.12 mm) and cell surface hydrophobicity (63%). Rhizosphere colonization by FAP1 was found 7.5 Log CFU g-1 of soil comparable with rhizoplane colonization (7.2 Log CFU g-1 of root). Therefore, biofilm formation on plant root

关键词： Biofilm PGPR Rhizosphere Colonization pseudomonas entomophila SEM and CLSM

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Common Preconditions for Safe Phage Therapy of pseudomonas aeruginosaInfections

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Advances in Microbiology 2014年第12期4卷 766-773页

作者： V. Krylov E. Pleteneva O. Shaburova M. Bourkaltseva S. Krylov E. Chesnokova O. Polygach Mechnikov Research Institute for Vaccines & Sera RAMS Moscow Russia

One of the approaches to the treatment of infections caused by multiply-(MDR) or extra drug- resistant (XDR) pathogenic strains may be application of bacterial viruses (bacteriophages)— phage therapy. Results of a long, but quite limited use of phage therapy in several Eastern European countries, as well as experiments on animal models in Western countries support the possibility to use phage therapy. However, given the role of phages in the evolution of bacterial pathogens, it is necessary to discuss and evaluate negative consequences of mass introduction of phage therapy, as the measures necessary for its safe use. We discuss some actions in case of transiting to world-wide use of phage therapy with purpose to prolong the active life of phage therapy and to diminish possible complications.

关键词： Phage Therapy Safety Measures pseudomonas aeruginosa

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Surveillance of Fluoroquinolone-Resistant Clinical Isolates of pseudomonas aeruginosa

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Open Journal of Medical Microbiology 2013年第2期3卷 144-150页

作者： Hideki Kobayashi Miho Isozaki Tatsuya Fukuda Yojiro Anzai Fumio Kato Pharmaceutical Unit Ohashi Medical Center Toho University Tokyo Japan School of Pharmaceutical Sciences Toho University Funabashi Chiba Japan

Ciprofloxacin (CPFX) and pazufloxacin (PZFX) have strong antibacterial activity against pseudomonas aeruginosa. We investigated the sensitivity of P. aeruginosa to CPFX and PZFX in 373 strains isolated from inpatients (321 strains) and outpatients (52 strains) during September 2010 to September 2011 at Toho University Ohashi Medical Center. The percentage of CPFX-non-susceptible (≥3.91 μg/mL) among inpatients was 22.4%, but that among outpatients was 1.9%. As the major resistance mechanism to fluoroquinolones in P. aeruginosa involves modification of type II topoisomerases (DNA gyrase and topoisomerase IV), we examined mutations in the quinolone-resistance-determining regions (QRDRs) of gyrA and parC of P. aeruginosa isolates. Among the 373 isolates, 73 isolates had reduced CPFX-susceptibility and 88 had reduced PZFX-susceptibility. Sequencing of gyrA and parC revealed base substitutions that resulted in amino acid replacements in QRDR of GyrA in 70 P. aeruginosa isolates, while Thr83Ile (in GyrA) and Ser87Leu (in ParC) substitutions were found in 12 strains. These replacements were clearly associated with reduced susceptibility to CPFX and PZFX. However, we also found strains with high MICs to quinolones without mutations in either gyrA or parC. We then investigated the effect of efflux pumps in CPFX-resistance in these isolates. In the presence of an efflux pump inhibitor, MIC values in 12 of 66 strains decreased to 1/23. We also sequenced genes related to overexpression of efflux pumps, viz., mexZ, mexR, and nfxB. Eight of the strains without mutations in QRDRs had a mutation in mexZ, 7 strains had a mutation in mexR, but no mutation was identified in nfxB.

关键词： pseudomonas aeruginosa Fluoroquinolone Resistant Type II Topoisomerases Efflux Pump

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Effect of Combined Therapy on Colistin Resistant pseudomonas aeruginosa: An in VitroStudy

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Advances in Microbiology 2019年第10期9卷 877-892页

作者： Nuzhath Fatima Amany Marwan Alkhansa Anwar Aymen Madhkhali Nahed Ali Areej Gadi Amany Hasnany Razan Hassan Hamali Mousa Ali Ayyashi Yahya Hobani Department of Medical Laboratory Technology College of Applied Medical Sciences Jazan University Jazan KSA College of Applied Medical Sciences Jazan University Jazan KSA Prince Mohammed Bin Nasser Hospital Jazan KSA

pseudomonas aeruginosa is known for its antibiotic resistance to the clinicians. The infections caused by this pathogen are hard to treat because of its highly versatile property to mutate and acquire drug resistance. pseudomonas aeruginosa also possesses intrinsic property of resistance to certain antibiotics like tetracyclines. However;in a practice to overcome the problem of multi drug resistance, clinicians restored the use of some antibiotics that were previously been used to treat the Pseudomonal infections;but they were discontinued because of its toxic effects. Colistin is an example of one such antibiotic. Use of Colistin was barred for its neurotoxicity. However in recent clinical trials, Colistin was reintroduced to fight with this superbug. Sadly in recent years, pseudomonas aeruginosa developed resistance to Colistin as well. Therefore combined therapy is an alternate and suitable treatment to overcome the infections caused by multidrug-resistant pseudomonas aeruginosa. The present study is an in vitro study;in which we tested synergy between two antibiotics namely streptomycin and Colistin on 29 clinical isolates of P. aeruginosa collected from hospitals in Jazan city KSA. The combination of two drugs showed synergistic activity on 55.1% of tested strains, while 20.6% strains had partial synergy, whereas indifferent synergy was observed in 13.8% strains and the 6.8% of strains had additive synergy. In addition to this, the drugs when combined also showed antagonism on one strain (3.44%). The present study showed synergistic action on Colistin-resistant pseudomonas aeruginosa to greater extent (55.1%) by the two tested drugs. Hence Colistin and streptomycin can be used as a suitable combination therapy (in vivo) to treat multidrug resistant P. aeruginosa infections.

关键词： “MDR”—Multidrug Resistant “MIC”—Minimum Inhibitory Concentration “FICI”—Fractional Inhibitory Concentration Index “MHB”—Muller Hinton Broth pseudomonas aeruginosa

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Polyhydroxyalkanoate Production by pseudomonas putidaKT217 on a Condensed Corn Solubles Based Medium Fed with Glycerol Water or Sunflower Soapstock

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Advances in Microbiology 2012年第3期2卷 241-251页

作者： Jeremy Javers Chinnadurai Karunanithy Agricultural and Biosystems Engineering Department South Dakota State University Brookings USA Biology and Microbiology Department South Dakota State University Brookings USA

pseudomonas putida KT217 was grown on a complex medium comprised of co-products of the ethanol and biodiesel industries to assess the organism's capability to produce medium-chain-length polyhydroxyalkanoate (mcl-PHA). The growth phase was carried out in a medium containing 400 g/L condensed corn solubles (CCS), supplemented with ammonium hydroxide as a nitrogen source. Following the exponential phase, co-products of the biodiesel industry (soapstock and glycerin) were fed into the reactor to trigger PHA production. When glycerin was added to the bioreactor (75 g/L total addition), the final cell dry weight (CDW) and PHA content were 30 g/L and 31%, respectively. The monomeric composition in the PHA formed was relatively uniform throughout incubation with 3-hydroxydecanoate dominating. When a total of 153 g/L of sunflower soapstock was added to the bioreactor in a fed-batch manner, the final CDW and PHA content were 17 g/L and 17%, respectively. Following addition of soapstock the monomeric composition of the polymer changed dramatically, with the 3-hydroxyoctanoate monomer becoming dominant and greater unsaturation present in the PHA.

关键词： Polyhydroxyalkanoate pseudomonas putida Condensed Corn Solubles Glycerol Soapstock

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pseudomonas aeruginosaBUP2—A Novel Strain Isolated from Malabari Goat Produces Type 2 Pyoverdine

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Advances in Bioscience and Biotechnology 2014年第11期5卷 874-885页

作者： Kizhakkepowathial Nair Unni Prakasan Priji Valerie A. Geoffroy Mukesh Doble Sailas Benjamin Enzyme Technology Laboratory Biotechnology Division Department of Botany University of Calicut Malappuram India Laboratoire de Microbiologie et de Génétique CNRS UPRES-A 7010 Université Louis-Pasteur Strasbourg France Department of Biotechnology Indian Institute of Technology Madras Chennai India

This study focuses on the isolation and characterization of a novel strain of siderophore producing bacterium, i.e., pseudomonas aeruginosa BUP2 (Pa BUP2) from the rumen of Malabari goat, coupled with qualitative and quantitative analyses of the siderophore produced by it. Pa BUP2—a facultative anaerobe was tuned to be an aerobe by repeatedly growing in Benjamin flask. The new isolate was grown in a specially designed semi-synthetic medium, designated as BUP medium, and the yellowish-green pigment produced was identified as a typical siderophore by spectrophotometry, Chromazurol-S assay, thin layer chromatography and isolectric focusing (IEF). The characteristic orange fluorescence upon UV irradiation on chromatogram and absorption maximum at λ404 confirmed that the characteristic siderophore produced by Pa BUP2 was a typical pyoverdine (PVD). This PVD was further categorized under type 2 by comparing its profile on the IEF gel with that of the representative strains of each PVD types, viz., Pa O1, Pa ATCC 27853 and Pa6. Moreover, the type 2 PVD was purified by XAD-4 Amberlite column chromatography and quantified;maximum yield (11.17 mg/ml) was observed on day 4 of incubation (37°C). Thus, it was confirmed that the bacterium isolated from the rumen content of Malabari goat is a novel strain of Pa capable of producing large quantity of PVD type 2 in specially designed BUP medium under aerobic condition, and that its clinical and industrial implications remain elusive.

关键词： pseudomonas aeruginosa BUP2 Siderophore Pyoverdine Type 2 Isoelectric Focusing

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Prevalence and Antimicrobial Susceptibility Profile of Metallo-β-Lactamase Producing pseudomonas aeruginosaIsolates at Kenyatta National Hospital

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Advances in Microbiology 2018年第11期8卷 885-893页

作者： Jane Njeri Karuitha Odera Susan Akinyi Maina Anne Njeri Mureithi Marianne Department of Microbiology School of Medicine University of Nairobi Nairobi Kenya

pseudomonas aeruginosa is a major cause of nosocomial infections with high mortality rates. The organism is highly resistant to most classes of drugs used and can develop resistance during treatment. One of the resistance mechanisms of P. aeruginosais is Metallo-β-Lactamase (MBL) production. MBL producing P. aeruginosa is a major health concern given it’s resistance to almost all available drugs. The prevalence of this resistant strain is unknown since there is no standardized method for testing MBL production. This was a laboratory based cross-sectional prospective study that was carried out from September 2015 to March 2016 at Kenyatta National Hospital. Ninety-nine isolates of P. aeruginosa were collected during the period and tested for antimicrobial susceptibility and isolates found to be resistant to imipenem tested for MBL production. The results indicated high resistance of P. aeruginosa to commonly used drugs. Of the isolates tested 69.7% were resistant to piperacillin, 63.6% were resistant to aztreonam, 58.6% were resistant to levofloxacin, 55.6% were resistant to cefipime, 65.7% were resistant to ceftazidime, 68.7% were resistant to ticarcillin-clavulanate, 72.2% were resistant to meropenem, 64.9% were resistance to imipenem while 86.4% of urine isolates were resistant to ofloxacin. Of the isolates resistant to imipenem 87.3% were found to be MBL producers. In conclusion, P. aeruginosais highly resistant to the drugs currently is used for treatment and resistance to carbapenems is largely due to MBL production.

关键词： pseudomonas aeruginosa Metallo-β-Lactamase Antimicrobial Resistance Kenyatta National Hospital

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Perturbation of Quorum Sensing in pseudomonas aeruginosaby Febuxostat

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Advances in Microbiology 2018年第8期8卷 650-664页

作者： Hisham Abdel Monem Abbas Wafaa Ezz Elarab Soliman Moutaz Ahamed Shaldam Department of Microbiology and Immunology Faculty of Pharmacy Zagazig University Zagazig Egypt Department of Microbiology and Biotechnology Faculty of Pharmacy Delta University for Science and Technology Mansoura Egypt Department of Medicinal Chemistry Faculty of Pharmacy Delta University for Science and Technology Mansoura Egypt

Quorum sensing is a signal-based communication system in bacteria. It is an attractive target because it regulates the production of virulence factors in pseudomonas aeruginosa. As a result, interference with quorum sensing could result in inhibition of virulence of pseudomonas aeruginosa with the merit of lack of selective pressure on growth that leads to development of resistance. This study investigated the anti-quorum sensing and anti-virulence activities of febuxostat in pseudomonas aeruginosa PAO1 strain. At 1/8 MIC of febuxostat, the production of the quorum-sensing regulated violacein pigment of Chromobacterium violaceum CV026 was significantly reduced. Moreover, it markedly reduced pyocyanin, hemolysin, protease and elastase production. Significant inhibitory activities were also found against biofilm, swimming, twitching and swarming motilities. Molecular docking showed the ability of febuxostat to inhibit quorum sensing by competing with the autoinducers to bind with LasR and RhlR receptors. Febuxostat could bind to both receptors by hydrogen bonding and hydrophobic interaction. From the Molecular docking scores, febuxostat is a very promising quorum sensing inhibitor. Febuxostat could also significantly decrease the level of expression of all QS genes LasI, LasR, RhlI, RhlR, PqsA and PqsR that regulate the production of virulene factors as confirmed by qRT-PCR.

关键词： Febuxostat pseudomonas aeruginosa Quorum Sensing Virulence Inhibition Molecular Docking

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Assessment of Susceptibility, Pharmacodynamics, and Therapeutic Response for Interpretation of Piperacillin-Tazobactam in VitroActivity in the Treatment of pseudomonas aeruginosaInfection

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Open Journal of Medical Microbiology 2012年第3期2卷 101-109页

作者： Toni-Marie Gonzalzles Mira Suseno Joseph M. Gaydos Paul C. Schreckenberger Carrie Sincak Maitry S. Mehta Lance R. Peterson Cleveland VAMC Cleveland USA Departments of Pathology and Medicine University of Chicago Chicago USA Departments of Pathology Medicine and Pharmacy NorthShore University HealthSystem (NorthShore) Evanston USA Loyola University Medical Center Maywood USA

pseudomonas aeruginosa remains an important pathogen. Our purpose was to determine the minimum inhibitory con-centration (MIC) and pharmacodynamic (PD) parameters predicting a positive response to therapy with piperacil-lin-tazobactam. Medical records were retrospectively reviewed at 3 centers. Data were recorded to assess age, type of disease, renal function, weight (body mass), MIC, antimicrobial treatment, and clinical outcome. Success was response to piperacillin-tazobactam alone, or in combination with another active agent;failure was lack of response. Of 78 eva-luable patients, 63 responded (7 UTI;56 non-UTI) and 15 did not;26 responding received combination therapy and 37 monotherapy. Piperacillin-tazobactam treatment was successful in 53 of 63 of non-UTI disease with a MIC of ≤64/4 μg/mL, but in only 3 of 7 with a MIC of >64/4 μg/mL (P = 0.023);overall 9 of 10 infections by strains with MICs = 32 - 64 μg/mL had a successful outcome. Piperacillin estimated time above MIC at 20% separated those responding from those that did not (P = 0.019).

关键词： pseudomonas Aeruginosa Piperacillin-Tazobactam Multidrug Resistance Pharmacodynamics

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