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H-NS family protein MvaU downregulates phenazine-1-carboxylic acid(PCA)biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium pseudomonas strain PA1201

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Synthetic and Systems Biotechnology 2021年第4期6卷 262-271页

作者： Yun-Ling Fang Ying Cui Lian Zhou Chitti Thawai Tatheer Alam Naqvi Hong-Yan Zhang Ya-Wen He State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesSJTU-Shanghai Nong Le Joint R&D Center on Biopesticides and BiofertilizersShanghai Jiao Tong University(SJTU)Shanghai200240China Department of Biology Faculty of ScienceKing Mongkut’s Institute of Technology LadkrabangBangkokThailand Department of Biotechnology COMSATS University IslamabadAbbottabad CampusAbbottabadPakistan Shanghai Nong Le Biological Products Company Limited Shanghai201419China

Histone-like nucleoid-structuring(H-NS)proteins are key regulators in gene expression silencing and in nucleoid *** H-NS family member proteins MvaU in pseudomonas aeruginosa are thought to bind the same AT-rich regions of chromosomes and function to coordinate the control of a common set of ***,we explored the molecular mechanism by which MvaU controls PCA biosynthesis in *** *** present evidence suggesting that MvaU is *** of mvaU significantly increased PCA production,and PCA production sharply decreased when mvaU was *** transcriptionally repressed phz2 cluster expression and consequently reduced PCA biosynthesis.β-galactosidase assays confirmed that base pairing near the􀀀35 box is required when MvaU regulates PCA production in *** mobility shift assays(EMSA)and additional point mutation analysis demonstrated that MvaU directly bound to an AT-rich motif within the promoter of the phz2 *** immunoprecipitation(ChIP)analysis also indicated that MvaU directly bound to the P5 region of the phz2 cluster *** repression of PCA biosynthesis was independent of QscR and OxyR in PA1201 and neither PCA or H2O2 were the environmental signals that induced mvaU *** findings detail a new MvaU-dependent regulatory pathway of PCA biosynthesis in PA1201 and provide a foundation to increase PCA fermentation titer by genetic engineering.

关键词： pseudomonas Phenazine-1-carboxylic acid phz cluster MvaU Regulatory network

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Effect of nalidixic acid on the morphology and protein expression of pseudomonas aeruginosa

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Asian Pacific Journal of Tropical Medicine 2012年第4期5卷 265-269页

作者： Saif Al Bahry Nallusamy Sivakumar Manal Al-Khambashi Department of Biology College of ScienceP.O.Box:36P.C.123Sultan Qaboos UniversitySultanate of Oman

Objective:To determine the effect of nalidixic acid on the morphology and protein expression of pseudomonas aeruginosa(***).Methods:Nalidixic acid solution of 1 600μg/mL was *** minimum inhibitory concentration(MIC) for *** was determined with tube dilution *** effect of nalidixic acid on the morphology of *** was studied using light microscope and scanning electron *** in protein profile were studied using ***:The MIC of nalidixic acid was 700μg/mL against *** exposure of *** to different concentrations of nalidixic acid resulted in deformation of most of the growing *** the concentration of 600μg/mL most of the cells turned into elongated and adhere to each other while some of the cells were *** intensity of protein bands were changed when they exposed to nalidixic ***:The present findings suggest that the morphology and protein expression of *** is greatly affected by nalidixic acid.

关键词： Nalidixic acid pseudomonas aeruginosa Deformed cells Scanning electron microscopy

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pseudomonas stutzeri 1317合成中长链聚羟基脂肪酸酯的组成分析模型

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高等学校化学学报 2002年第1期23卷 71-74页

作者：徐军贺文楠郭宝华胡平张增民张广林会兰陈金春陈国强清华大学材料科学与工程研究院化工系高分子研究所北京100084 清华大学生物科学与技术系北京100084

pseudomonasstutzeri1 31 7可以在不同碳源上生长 ,合成不同共聚单体单元组成的中长链聚羟基脂肪酸酯 ( mcl PHAs) .本文在已知的代谢途径的基础上 ,提出了关于 mcl PHAs组成的数学模型 ,结果表明 ,mclPHAs的共聚单体单元组成主要取决... 详细信息

pseudomonasstutzeri1 31 7可以在不同碳源上生长 ,合成不同共聚单体单元组成的中长链聚羟基脂肪酸酯 ( mcl PHAs) .本文在已知的代谢途径的基础上 ,提出了关于 mcl PHAs组成的数学模型 ,结果表明 ,mclPHAs的共聚单体单元组成主要取决于前体生成途径 ,以及该途径和聚合途径酶对不同碳原子数前体的选择性 .实验结果与文献报道结果均验证了该模型 .代谢控制分析的结果表明 ,碳数为 8～ 1 0的前体的聚合效率最高 .通过调制前体生成途径 ,可以有效调节

关键词：中长链聚羟基脂肪酸酯 pseudomonas stutzeri 数学模型单体单元组成代谢控制分析生物合成

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Antibacterial activities of some plant extracts alone and in combination with different antimicrobials against multidrug-resistant pseudomonas aeruginosa strains

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Asian Pacific Journal of Tropical Medicine 2010年第4期3卷 266-269页

作者： Ghaleb Adwan Bassam Abu-Shanab Kamel Adwan Department of Biology and Biotechnology An-Najah N.UniversityP.O.Box(7)-NablusPalestine Faculty of Veterinary Medicine An-Najah N.UniversityNablusPalestine

Objective:To evaluate the possible in vitro interaction between ethanolic extracts of Rhus coriaria[***)(seed),Sacropoterium spinosum(***)(seed),Rosa damascena(R. damascene)(flower) and certain known antimicrobial drugs including oxytetracycline HCl, penicillin C,cephalexin,sulfadimethoxine as sodium,and enr of *** synergy study was carried out against 3 clinical strains of multidrug-resistant pseudomonas aeruginosa (***).Methods:Evaluation of synergy interaction between plant extracts and antimicrobial agents was carried out using microdilution ***:The results of this study showed that there is a decrease in the MIC in case of combination of ethanolic plant extracts and test antimicrobial *** most interesting result was that the combination between R. coriaria and these antibiotics,showed a high decrease in minimum inhibitory concentration(MIC), and a strong bactericidal activity against these ***:These results may indicate that combinations between *** extract and these antibiotics could be useful in fighting emerging drug-resistance ***,which may due to that *** extract contain natural inhibitors working by different mechanisms or inhibiting efflux *** we have experiments underway leading to the identification of the active molecules present in ***,in vivo experiments are needed to confirm pseudomonal protection.

关键词： Synergism Rhus coriaria Sacropoterium spinosum Rosa damascena Medicinal plants Antimicrobial agents pseudomonas aeruginosa Palestine

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Phenolic compounds affect production of pyocyanin, swarming motility and biofilm formation of pseudomonas aeruginosa

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Asian Pacific Journal of Tropical Biomedicine 2016年第8期6卷 698-701页

作者： Aylin Ugurlu Aysegul Karahasan Yagci Seyhan Ulusoy Burak Aksu Gulgun Bosgelmez-Tinaz Department of Microbiology School of Medicine Marmara University Department of Biology Faculty of Arts and Sciences Süleyman Demirel University Department of Basic Pharmaceutical Sciences Faculty of Pharmacy Marmara University

Objective: To investigate the effects of plant-derived phenolic compounds(i.e. caffeic acid, cinnamic acid, ferulic acid and vanillic acid) on the production of quorum sensing regulated virulence factors such as pyocyanin, biofilm formation and swarming motility of pseudomonas aeruginosa(P. aeruginosa) ***: Fourteen clinical P. aeruginosa isolates obtained from urine samples and P. aeruginosa PA01 strain were included in the study. The antibacterial effects of phenolic compounds were screened by well diffusion assay. Pyocyanin and biofilm activity were measured from culture supernatants and the absorbance values were measured using a spectrophotometer. Swarming plates supplemented with phenolic acids were point inoculated with P. aeruginosa strains and the ability to swarm was determined by measuring the distance of swarming from the central inoculation ***: Tested phenolic compounds reduced the production of pyocyanin and biofilm formation without affecting growth compared to untreated cultures. Moreover, these compounds blocked about 50% of biofilm production and swarming motility in P. aeruginosa ***: We may suggest that if swarming and consecutive biofilm formation could be inhibited by the natural products as shown in our study, the bacteria could not attach to the surfaces and produce chronic infections. Antimicrobials and natural products could be combined and the dosage of antimicrobials could be reduced to overcome antimicrobial resistance and drug side effects.

关键词： Quorum sensing Phenolic compounds pseudomonas aeruginosa Biofilm Swarming

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Isolation of an antibiotic producer pseudomonas *** the Persian Gulf

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Asian Pacific Journal of Tropical Medicine 2010年第4期3卷 318-321页

作者： Esmaeil Darabpour Mohammad Roayaei Ardakani Hossein Motamedi Gholamreza Ghezelbash Mohammad Taghi Ronagh Department of Biology Faculty of ScienceShahid Chamran UniversityAhvazIran Department of Marine Ecology Marine Science & Technology UnivesityKhoramshahrIran

Objective:To investigate the isolation of marine antibiotic-producing bacteria from the Persian Gulf,as an untapped source for searching new natural ***:Initially water and sediment samples were collected from 18 study sites in the some northern areas of Persian *** of the bacterial isolates using Marine Agar 2216 were inoculated into Marine broth and incubated on a rotary shaker at 28℃for 2-7 *** of their ethyl acetate extract was assessed at 100 mg/mL concentration in disc diffusion method against 6 gram-positive and 5 gram-negative *** antibiotics were used as ***:Altogether,46 bacterial colonies were *** one isolate from a marine sediment sample collected at a depth of 10 m,identified as pseudomonas aeruginosa PG-01,was exhibited the capability of antibiotic *** obtained raw extract from intended bacterium was effective against all tested gram positive bacteria while gram negative bacteria were *** resisitant Stapuylococcus aureus(MRSA),Streptococcus pyogenes(***).Staphylococcus epidermidis (***) and Bacillus cereus(***) were the most sensitive *** of tested pathogens were multidrug *** antibacterial compound from this bacterium was active even at 120℃.The optimized temperature and time for antibacterial metabolite production were 37℃and 72 hrs,***:Considering the antibacterial effect of pseudomonas aeruginosa PG-01 especially against MRSA,it can be regarded the intended bacterium as a valuable strain and can give hope for treatment of diseases caused by multidrug resistant bacteria.

关键词： Persian Gulf Marine sediment pseudomonas Antibacterial compound MRSA

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Studies on Locusts' Pathogenesis and Energy Metabolic Inhibition Induced by the Insecticidal Protein Purified from pseudomonas Pseudoalcaligenes

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High Technology Letters 2002年第4期8卷 13-17页

作者：赵建 Zhu Jianqing Luo Xia Liu Zhu Chen Donghui Yang Zhirong National Lab of Biocontrol Engineering of Grassland Pests Sichuan University Chengdu 610065 P.R.China

The insecticidal protein produced by pseudomonas pseudoalcaligenes is purified from the suspension of the bacterial culture. As an intact molecule, the protein acts on the foregut, midgut, hindgut, vasa Malpighii and fat body, and kills locusts. The disinsection rates of feeding and injection are 63.3% and 65.7%, respectively. After 24h to 48h, it is observed that all these tissues and cells show pathological changes in varying degrees, and so did the host cellular organs of these cells, such as cytoblast, mitochondria, endoplasmic reticulum and ribosome. Particularly, the changes of the mitochondria are much more serious than those of others. Detection of oxygen electrode shows the efficiency of oxidative phosphorylation and the ATP synthesis decreases, while the activity of mitochondrial ATPase is almost not affected. That means the energy utilization of locusts is in gear, but the shortage of the supplying of energy results in their death. This research forms the substantial basis for controlling locusts.

关键词： pseudomonas pseudoalcaligenes, insecticidal protein, pathogenesis, mitochondria, oxidative phosphorylation

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Biodegradation of Petroleum Hydrocarbon by pseudomonas Aeruginosa

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China Petroleum Processing & Petrochemical Technology 2012年第2期14卷 66-70页

作者： Chen Boshui Zhang Nan Wu Jiang Wang Jiu Fang Jianhua Department of Petrochemistry Logistical Engineering UniversityChongqing 401311

A bacterial strain of the genus pseudomonas aeruginosa was inoculated into a hydrocarbon culture medium and incubated for a definite period of time. The ability of the bacterial strain to biodegrade a hydrocarbon, viz. n-hexadecane, was evaluated through determining the hexadecane concentration in the inoculated culture medium on a gas chromatograph (GC). The effect of pH value on the degrading ability of the bacterial isolate and the impact of temperature on microbial growth were also explored. Test results showed that pseudomonas aeruginosa was markedly effective in biodegrading n-hexadecane. Furthermore, the ability of pseudomonas aeruginosa to biodegrade n-hexadecane was different at various pH values. pseudomonas aeruginosa provided excellent degrading ability at a pH value of 7.0. The microbial cells of pseudomonas aeruginosa increased with an increasing incubation duration at temperatures ranging from 28 ℃ to 35 ℃, and an exponential phase of microbial growth was observed.

关键词：铜绿假单胞菌生物降解能力石油烃 pseudomonas 微生物生长正十六烷持续时间温度范围

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STUDIES ON A BACTERIOCIN AND ITS SUBUNITS PRODUCED BY pseudomonas solanacearum M2

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Chinese Science Bulletin 1991年第4期36卷 339-344页

作者：王明波张向东范云六 Laboratory of Molecular Biology Biotechnology Research Center Chinese Academy of Agricultural Sciences Beijing 100081 PRC

Ⅰ. INTRODUCTIONBacteriocins are produced by many strains of bacteria including plant pathogens. The potential value of bacteriocins as a biological control agent of plant pathogens is being recognized. However, only ... 详细信息

关键词： pseudomonas solanacearum bacteriocin purification of protein subunits amino acid sequence bacteriocidal activity.

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Immunogenic efficacy of differently produced recombinant vaccines candidates against pseudomonas aeruginosa infections

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第二军医大学学报 1998年第S1期19卷 98-103页

作者： Von Specht BU Gabelsberger J△ Knapp B▲ Hundt E▲ SchmidtPilger H Bauernsachs S△Lenz U▲ Domdey H△ From Chirurgische Universitatsklinik der Universitat Freiburg Freiburg Germany Institut fur Biochemie-Genzentrum-der LMU Munchen Munchen Germany Chiron Behring GmbH Marberg Germany

Ｐｓｅｕｄｏｍｏｎａｓａｅｒｕｇｉｎｏｓａｉｓａｃｏｍｍｏｎｃａｕｓｅｏｆｉｌｎｅｓｓａｎｄｄｅａｔｈｉｎｉｍｍｕｎｏｃｏｍｐｒｏｍｉｓｅｄｐａｔｉｅｎｔｓ．Ｉｎｐａｒｔｉｃｕｌａｒ，ｔｈｅｒｉｓｋｔｏｔｈｏｓｅｓｕ... 详细信息

Ｐｓｅｕｄｏｍｏｎａｓａｅｒｕｇｉｎｏｓａｉｓａｃｏｍｍｏｎｃａｕｓｅｏｆｉｌｎｅｓｓａｎｄｄｅａｔｈｉｎｉｍｍｕｎｏｃｏｍｐｒｏｍｉｓｅｄｐａｔｉｅｎｔｓ．Ｉｎｐａｒｔｉｃｕｌａｒ，ｔｈｅｒｉｓｋｔｏｔｈｏｓｅｓｕｆｅｒｉｎｇｆｒｏｍｓｅ－ｖｅｒｅ...

关键词： pseudomonas aeruginosa outer membrane protein vaccine

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