Isolation of an antibiotic producer Pseudomonas *** the Persian Gulf

作     者：Esmaeil Darabpour Mohammad Roayaei Ardakani Hossein Motamedi Gholamreza Ghezelbash Mohammad Taghi Ronagh 

作者机构：Department of BiologyFaculty of ScienceShahid Chamran UniversityAhvazIran Department of Marine EcologyMarine Science & Technology UnivesityKhoramshahrIran 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2010年第3卷第4期

页      面：318-321页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：the vice chancellor for research of Shahid Chamran University Ahvaz Iran for the research grant and financial support 

主　　题：Persian Gulf Marine sediment Pseudomonas Antibacterial compound MRSA 

摘      要：Objective:To investigate the isolation of marine antibiotic-producing bacteria from the Persian Gulf,as an untapped source for searching new natural ***:Initially water and sediment samples were collected from 18 study sites in the some northern areas of Persian *** of the bacterial isolates using Marine Agar 2216 were inoculated into Marine broth and incubated on a rotary shaker at 28℃for 2-7 *** of their ethyl acetate extract was assessed at 100 mg/mL concentration in disc diffusion method against 6 gram-positive and 5 gram-negative *** antibiotics were used as ***:Altogether,46 bacterial colonies were *** one isolate from a marine sediment sample collected at a depth of 10 m,identified as Pseudomonas aeruginosa PG-01,was exhibited the capability of antibiotic *** obtained raw extract from intended bacterium was effective against all tested gram positive bacteria while gram negative bacteria were *** resisitant Stapuylococcus aureus(MRSA),Streptococcus pyogenes(***).Staphylococcus epidermidis (***) and Bacillus cereus(***) were the most sensitive *** of tested pathogens were multidrug *** antibacterial compound from this bacterium was active even at 120℃.The optimized temperature and time for antibacterial metabolite production were 37℃and 72 hrs,***:Considering the antibacterial effect of Pseudomonas aeruginosa PG-01 especially against MRSA,it can be regarded the intended bacterium as a valuable strain and can give hope for treatment of diseases caused by multidrug resistant bacteria.