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DIW1 encoding a cladeⅠPP2C phosphatase negatively regulates drought tolerance by de-phosphorylating TaSnRK1.1 in wheat

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Journal of Integrative Plant Biology 2023年第8期65卷 1918-1936页

作者： Jingyi Wang Chaonan Li Long Li Lifeng Gao Ge Hu Yanfei Zhang Matthew P.Reynolds Xueyong Zhang Jizeng Jia Xinguo Mao Ruilian Jing National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China International Maize and Wheat Improvement Center Texcoco 56237Mexico

Drought seriously impacts wheat production(Triticum aestivum L.),while the exploitation and utilization of genes for drought tolerance are insufficient.Leaf wilting is a direct reflection of drought tolerance in plants.Clade A PP2Cs are abscisic acid(ABA)co-receptors playing vital roles in the ABA signaling pathway,regulating drought response.However,the roles of other clade PP2Cs in drought tolerance,especially in wheat,remain largely unknown.Here,we identified a gain-of-function drought-induced wilting 1(DIW1)gene from the wheat Aikang 58 mutant library by map-based cloning,which encodes a cladeⅠprotein phosphatase 2C(TaPP2C158)with enhanced protein phosphatase activity.Phenotypic analysis of overexpression and CRISPR/Cas9 mutant lines demonstrated that DIW1/TaPP2C158 is a negative regulator responsible for drought resistance.We found that TaPP2C158 directly interacts with TaSnRK1.1 and de-phosphorylates it,thus inactivating the TaSnRK1.1–Ta AREB3 pathway.TaPP2C158 protein phosphatase activity is negatively correlated with ABA signaling.Association analysis suggested that C-terminal variation of TaPP2C158 changing protein phosphatase activity is highly correlated with the canopy temperature,and seedling survival rate under drought stress.Our data suggest that the favorable allele with lower phosphatase activity of TaPP2C158 has been positively selected in Chinese breeding history.This work benefits us in understanding the molecular mechanism of wheat drought tolerance,and provides elite genetic resources and molecular markers for improving wheat drought tolerance.

关键词： association analysis drought elite genetic resources map-based clone PP2C protein phosphatase wheat wilting

Suppression of ETI by PTI priming to balance plant growth and defense through an MPK3/MPK6-WRKYs-PP2Cs module

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Molecular Plant 2023年第5期16卷 903-918页

作者： Dacheng Wang Lirong Wei Ting Liu Jinbiao Ma Keyi Huang Huimin Guo Yufen Huang Lei Zhang Jing Zhao Kenichi Tsuda Yiming Wang Key Laboratory of Biological Interactions and Crop Health Department of Plant PathologyNanjing Agricultural UniversityNanjing 210095China Key Laboratory of Integrated Management of Crop Disease and Pests Ministry of EducationNanjing Agricultural UniversityNanjingChina State Key Laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryHubei Key Lab of Plant PathologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

Pattern-triggered immunity(PTI)and effector-triggered immunity(ETI)are required for host defense against pathogens.Although PTI and ETI are intimately connected,the underlying molecular mechanisms remain elusive.In this study,we demonstrate that flg22 priming attenuates Pseudomonas syringae pv.tomato DC3000(Pst)AvrRpt2-induced hypersensitive cell death,resistance,and biomass reduction in Arabidopsis.Mitogen-activated protein kinases(MAPKs)are key signaling regulators of PTI and ETI.The absence of MPK3 and MPK6 significantly reduces pre-PTI-mediated ETI suppression(PES).We found that MPK3/MPK6 interact with and phosphorylate the downstream transcription factor WRKY18,which regulates the expression of AP2C1 and PP2C5,two genes encoding protein phosphatases.Furthermore,we observed that the PTI-suppressed ETI-triggered cell death,MAPK activation,and growth retardation are significantly attenuated in wrky18/40/60 and ap2c1 pp2c5 mutants.Taken together,our results suggest that the MPK3/MPK6-WRKYs-PP2Cs module underlies PES and is essential for the maintenance of plant fitness during ETI.

关键词： PAMP-triggered immunity effector-triggered immunity MAPK,WRKY18 protein phosphatase

Melatonin ameliorated okadaic-acid induced Alzheimer-like lesions

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Acta Pharmacologica Sinica 2004年第3期25卷 22-26页

作者： Yi-peng WANG,Xian-tao LI,Shi-jie LIU,Xin-wen ZHOU,Xiao-chuan WANG,Jian-zhi WANG2 TongjiMedical College,Huazhong University of Science and Technology,Wuhan 430030,China Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

AIM: To explore the protective effects of melatonin (Mel) on the abnormal phosphorylation of neuronal cytoskeletal proteins. METHODS: We generated a neuroblastoma (SH-SY5Y) cell system in which cytoskeletal proteins are abnormally phosphorylated resulting in microtubule disruption due to the marked inhibition of protein phosphatase activities by okadaic acid (OA). RESULTS: OA-induced declines in cell viability and mitochondrial metabolic activity were remarkably prevented by Mel. In addition, the hyperphosphorylation/accumulation of neurofilament- (NF-) H/M subunits and the disruption of microtubules, induced by OA, were significantly inhibited by Mel. CONCLUSION: Our results suggest multiple protective functions of Mel against a series of pathological lesions known to culminate in AD, including abnormal phosphorylation of cytoskeletal proteins, microtubule disassembly and mitochondrion-initiated cell toxicity.

关键词： Alzheimer disease protein phosphatase okadaic acid neurofilament proteins melatonin

ABA Signaling in Guard Cells Entails a Dynamic protein-protein Interaction Relay from the PYL-RCAR Family Receptors to Ion Channels

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Molecular Plant 2013年第2期6卷 528-538页

作者： Sung Chul Lee Chae Woo Lim Wenzhi Lan Kai He Sheng Luan Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA School of Biological Sciences (BK21 Program) Chung-Ang University Seoul 156-756 Korea Plant Molecular Biology Institute and College of Life Sciences Nanjing University China WCU Program Chonnam National University Gwangju Korea

Plant hormone abscisic acid （ABA） serves as an integrator of environmental stresses such as drought to trig-ger stomatal closure by regulating specific ion channels in guard cells. We previously reported that SLAC1, an outward anion channel required for stomatal closure, was regulated via reversible protein phosphorylation events involving ABA signaling components, including protein phosphatase 2C members and a SnRK2-type kinase （OST1）. In this study, we reconstituted the ABA signaling pathway as a protein-protein interaction relay from the PYL/RCAR-type receptors, to the PP2C-SnRK2 phosphatase-kinase pairs, to the ion channel SLAC1. The ABA receptors interacted with and inhibited PP2C phosphatase activity against the SnRK2-type kinase, releasing active SnRK2 kinase to phosphorylate, and activate the SLAC1 channel, leading to reduced guard cell turgor and stomatal closure. Both yeast two-hybrid and bimolecular fluorescence complementation assays were used to verify the interactions among the components in the pathway. These biochemical assays demonstrated activity modifications of phosphatases and kinases by their interaction partners. The SLAC1 channel activity was used as an endpoint readout for the strength of the signaling pathway, depending on the presence of different combinations of signaling components. Further study using transgenic plants overexpressing one of the ABA receptors demonstrated that changing the relative level of interacting partners would change ABA sensitivity.

关键词： abscisic acid ABA receptor protein kinase protein phosphatase SLAC1.

PAPP2C Interacts with the Atypical Disease Resistance protein RPW8.2 and Negatively Regulates Salicylic Acid-Dependent Defense Responses in Arabidopsis

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Molecular Plant 2012年第5期5卷 1125-1137页

作者： Wen-Ming Wang Xian-Feng Ma Yi Zhang Ming-Cheng Luo Guo-Liang Wang Maria Bellizzi Xing-Yao Xiong Shun-Yuan Xiao Institute for Bioscience and Biotechnology Research and Department of Plant Sciences and Landscape Architecture University of Maryland Rockville MD20850 USA Rice Research Institute Sichuan Agricultural University Chengdu 611130 China Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop Hunan Agricultural University Changsha 410128 China Department of Plant Sciences University of.Califor.nia. Davis: CA 95616 USA Department of Plant Pathology The Ohio State University Columbus OH 43210 USA

Many fungal and oomycete pathogens differentiate a feeding structure named the haustorium to extract nutrition from the plant epidermal cell. The atypical resistance （R） protein RPW8.2 activates salicylic acid （SA）-dependent, haustorium-targeted defenses against Golovinomyces spp., the causal agents of powdery mildew diseases on multiple plant species. How RPW8.2 activates defense remains uncharacterized. Here, we report that RPWS.2 interacts with the phytochrome-associated protein phosphatase type 2C （PAPP2C） in yeast and in planta as evidenced by co- immunoprecipitation and bimolecular fluorescence complementation assays. Down-regulation of PAPP2C by RNA interfer- ence （RNAi） in Col-0 plants lacking RPWS.2 leads to leaf spontaneous cell death and enhanced disease resistance to powdery mildew via the SA-dependent signaling pathway. Moreover, down-regulation of PAPP2C by RNAi in the RPW8.2 background results in strong HR-like cell death, which correlates with elevated RPWS.2 expression. We further demonstrate that hemagglutinin （HA）-tagged PAPP2C prepared from tobacco leaf cells transiently transformed with HA-PAPP2C possesses phosphatase activity. In addition, silencing a rice gene （Os04g0452000） homologous to PAPP2C also results in spontaneous cell death in rice. Combined, our results suggest that RPW8.2 is functionally connected with PAPP2C and that PAPP2C negatively regulates SA-dependent basal defense against powdery mildew in Arabidopsis.

关键词： Arabidopsis PAPP2C RPW8 protein phosphatase powdery mildew resistance SA-signaling.

Cautionary Notes on the Usage of abil-2 and abil-3 Mutants of Arabidopsis ABI1 for Functional Studies

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Molecular Plant 2015年第2期8卷 335-338页

作者： Yaorong Wu Yaqiong Li Yongchang Liu Qi Xie State Key Laboratory of Plant Genomics National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences No. 1 West Beichen Road Chaoyang District Beijing 100101 China College of Agriculture/Key Laboratory of Oasis Ecology Agriculture of BINTUAN Shihezi University Shihezi 832003 China

Dear Editor,Abscisic acid （ABA） is a key hormone involved in plant growth and development, as well as stress adaptation. Recently, a core ABA signal pathway was proposed in which the ABA receptor proteins （PYR/PYL/RCAR） bind and inhibit type 2C protein phosphatases （PP2Cs） in the presence of ABA, which in turn releases the SnRK2s kinases, and the phosphorylated SnRK2s accumulate and subsequently phosphorylate the ABA-responsive element binding factors （Melcher et al., 2009; Cutler et al., 2010）.

关键词： ABSCISIC-ACID protein phosphatase THALIANA