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Journal of Integrative Agriculture 2020年第9期19卷 2294-2300页

作者： ZENG Xian-ying CHEN Xiao-han MA Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua SHI Jian-zhong CHEN Pu-cheng JIANG Yong-ping LI Yan-bing HU Jing-lei LU Tong MAO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin CHEN Hua-lan State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China Harbin Weike Biotechnology Development Company Harbin 150069P.R.China

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.***.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.***.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.

关键词： avian influenza H5/H7 trivalent vaccine Re-11 Re-12 and H7-Re2 strains protective efficacy

protective efficacy of an H5/H7 trivalent inactivated vaccine(H5-Re13,H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses

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Journal of Integrative Agriculture 2022年第7期21卷 2086-2094页

作者： ZENG Xian-ying HE Xin-wen MENG Fei MA Qi WANG Yan BAO Hong-mei LIU Yan-jing DENG Guo-hua SHI Jian-zhong LI Yan-bing TIAN Guo-bin CHEN Hua-lan State Key Laboratory of Veterinary Biotechnology National Poultry Laboratory Animal Resource CenterHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.***.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.***.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.***.4.4 b HA gene, an H5 N6 virus bearing the clade 2.***.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.***.4.4 h or 2.***.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature.

关键词： avian influenza H5/H7 trivalent vaccine H5-Re13 H5-Re14 H7-Re4 strains protective efficacy chickens ducks and geese

Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China

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Science China(Life Sciences) 2022年第4期65卷 795-808页

作者： Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin150069China College of Agronomy Liaocheng UniversityLiaocheng252000China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou510642China

The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza.

关键词： avian influenza virus H5N8 evolution pathogenicity antigenicity vaccine protective efficacy

Immunogenicity and protective efficacy of Vibrio harveyi pcFlaA DNA vaccine in Epinephelus awoara

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Chinese Journal of Oceanology and Limnology 2009年第4期27卷 769-774页

作者：覃映雪苏永全王世峰鄢庆枇 Fisheries College Jimei University Department of Oceanography Xiamen University Ocean College of Hainan University

The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1（＋）（designated as pcFlaA）. Ninety grouper （Epinephelus awoara） were separated into three equal size groups. An experimental group was immunized with pcFlaA, Control I group was immunized with the vector pcDNA3.1（＋）, and Control 1I group was immunized with PBS. The expression of pcFlaA mRNA and protein was examined using reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry. We also evaluated the immunogenicity and protective efficacy of pcFlaA against V. harveyi by measuring the lymphocyte proliferation response and serum levels of specific antibody and conducting a bacterial challenge test. We successfully transfected the fish muscle with pcFlaA. The pcFlaA mRNA and protein was expressed in the muscle cells for up to one month following injection. The proliferation response of lymphocytes in fish immunized with pcFlaA was significantly higher than in control group II. Furthermore, the immunized fish generated specific antibody. The vaccination also resulted in significantly higher survival during the bacterial challenge test.

关键词： Vibrio harveyi DNA vaccination immunogenicity protective efficacy

H2 strain attenuated live hepatitis A vaccines:protective efficacy in a hepatitis A outbreak

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World Journal of Gastroenterology 2000年第6期6卷 829-832页

作者： Yu Liang Zhao Zong Da Meng Zhi Yi Xu Jun Jie Guo Shao Ai Chai Cheng Gang Duo Xuan Yi Wang Jin Feng Yao Hong Bin Liu Shun Xiang Qi Hui Bin Zhu Department of Virology Hebei Provincial Sanitary and Antiepidemic StationBaoding 071000Hebei ProvinceChina Department of Epidemiology Shanghai Medical University.Shanghai 200032China Fucheng County Sanitary and Anti-epidemic Station Zhejiang Academy of Medical Sciences Hangzhou

AIM:To investigate the protective efficacy of H2 strain attenuated live hepatitis A vaccines (H2-strain vaccines) in hepatitis A (HA) ***:With the permission of their parents, 5551 pre-school and grade 1-3 primary school children were inoculated with 1 dose (10(6.5) TCID(50)) of H2 strain vaccines in a nonrandomized, controlled trial conducted in Fucheng County, Hebei Province in May *** 6485 children in the same grades and compatible in gender and age were enrolled as controls. Epidemiological and serological survey was conducted to evaluate the protective efficacy of the vaccines. ELISA was used to detect serum IgM ***:HA outbreak started in early May 1998, peaked in the middle of the same month, and lasted about 80 days. Overall 302 HA cases were found, 192(63.58%) were 5-9 years old. One vaccinee and 25 control cases were found to have hepatitis A, which account for 0.28% (1/356) and 5.92% (25/422) of all vaccinees and controls in the 14 villages, respectively. The protective efficacy of vaccines was 95.27% (95% CI: 85.83%-104.72%). In subjects tested for anti-HAV IgM from 13 villages, 1(0.40%) overt and 11(4.06%) asymptomatic HAV cases were found in 271 vaccinees but 21(6.69%) of overt and asymptomatic ones were found in 314 ***:H2 strain vaccines were excellent in preventing overt hepatitis A,but not so effective in preventing asymptomatic hepatitis A virus infection.A booster dose might be needed to get permanent reliable immunity.

关键词： hepatitis A/prevention and control vaccines, attenuated vaccines, inactivated viral hepatitis vaccines disease outbreaks protective efficacy

Immunogenicity and protective efficacy of an inactivated SFTS vaccine candidate in mice

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Biosafety and Health 2022年第1期4卷 45-52页

作者： Aqian Li Xinxian Dai Lei Chen Lin Liu Chuan Li Yang Liu Wei Wu Xiaoxia Huang Jiandong Li Shiwen Wang Mifang Liang Xiuling Li Dexin Li Department of Viral Hemorrhagic Fever National Institute for Viral Disease Control and PreventionChina CDCBeijing 102206China b National Vaccine and Serum Institute Beijing 102206China Suzhou Institute of Systems Medicine Suzhou 215123China China CDC-WIV Joint Research Center for Emerging Diseases and Biosafety Center for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China

Severe fever with thrombocytopenia syndrome(SFTS),caused by a novel identified bunyavirus SFTS virus(SFTSV),was an emerging viral infectious disease that was firstly reported in *** are no licensed vaccines and therapeutics against SFTSV currently.B‐Propiolactone(BPL)inactivated whole virions of SFTSV strain AH12 were prepared as experimental vaccine in different antigen dose with or without Al(OH)3 *** experimental SFTS vaccine was a satisfying immunogen,which could efficiently trigger the development of high levels of SFTSV NP‐specific IgG antibodies and neutralizing antibodies against SFTSV Strain HB29 in BALB/c and C57/BL6 mice,and could induce SFTS virus‐specific cellular immune responses to a certain extent.A single dose of vaccine was immunogenically insufficient in BALB/c mice;the second and third dose resulted in significant boost in antibody *** use of Al(OH)3 adjuvant resulted in higher antibody *** mediate‐dose of vaccine could induce as high and equivalent level of antibody titer as that of high‐*** experimental SFTS vaccine in mediate‐and high antigen dose with adjuvant resulted in solid protection of C57/BL6 mice against wild‐type SFTSV challenge with markedly accelerated virus clearance from blood and spleen compared with *** experimental SFTS vaccine prepared in this study could efficiently elicit virus specific humoral and cellular immune responses in both BALB/c and C57/BL6 mice,and could protect C57/BL6 mice against SFTS virus *** results supplied evidence that inactivated vaccine was a promising vaccine candidate for the prevention of SFTSV infection.

关键词： SFTS virus Inactivated vaccine Immunogenicity protective efficacy

Study on Immunogenicity and protective efficacy of a new vaccine expressing Ag85B and MPT83 fusion protein from Mycobacterium tuberculosis

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Study on Immunogenicity and protective efficacy of a new vac...

A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs

第七届全国医学生物化学与分子生物学和第四届全国临床应用生物化学与分子生物学联合学术研讨会暨医学生化分会会员代表大会

作者： Yu Ting Bao Hong Jin Yufen Chang Jing Clinical Labortary of No.2 Hospital of Jilin University

Genetic immunity is a new promising approach for the development of novel tuberculosis *** this study,it is shown that DNA vaccines expressing the fusion protein of antigen 85B(Ag85B) and antigenic target antigen MPT83 can induce high levels of specific IgG2a antibody subtype in the mice,the ratio of antibody subtypes IgG2a to IgG1 changes 4weeks after immunization,and IgG1 is gradually shifted to the main antibody *** vaccines also elicit cellular immunity as shown by specific spleen lymphocytes proliferation to Ag85B or MPT83 protein and the production of high levels of IFN-g and IL-2, which is similar to that elicited by *** of mice with DNA vaccines expressing the fusion protein Ag85B-MPT83 results in a significant level of protection against the subsequent highdose challenge with virulent Mycobacterium tuberculosis(MTB) *** reducing degree of mycobacterial loads in the organ of mice immunized with recombinant plasmid is more than that of BCG,through the analysis of result,we may conclude that the protective effect provided by DNA vaccine expressing the Ag85B-MPT83 fusion protein is much more effective than that afforded by the classical BCG.

关键词： Mycobacterium tuberculosis Ag85b MPT83 Immunogenicity protective efficacy vaccine

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Science China(Life Sciences) 2020年第5期63卷 623-634页

作者： Weiye Chen Dongming Zhao Xijun He Renqiang Liu Zilong Wang Xianfeng Zhang Fang Li Dan Shan Hefeng Chen Jiwen Zhang Lulu Wang Zhiyuan Wen Xijun Wang Yuntao Guan Jinxiong Liu Zhigao Bu State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150001China National High Containment Laboratory for Animal Diseases Control and Prevention Harbin 150069China

African swine fever(ASF)is a devastating infectious disease in swine that is severely threatening the global pig *** efficacious vaccine is urgently ***,we used the Chinese ASFV HLJ/18 as a backbone and generated a series of genedeleted *** virulence,immunogenicity,safety,and protective efficacy evaluation in specific-pathogen-free pigs,commercial pigs,and pregnant sows indicated that one virus,namely HLJ/18-7GD,which has seven genes deleted,is fully attenuated in pigs,cannot convert to the virulent strain,and provides complete protection of pigs against lethal ASFV *** study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV,and as such is expected to play an important role in controlling the spread of ASFV.

关键词： African swine fever vaccine safety protective efficacy pig

Towards development of a universal dengue vaccine-how close are we?

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Asian Pacific Journal of Tropical Medicine 2017年第3期10卷 209-218页

作者： Ee Leen Pang Hwei-San Loh School of Biosciences Faculty of ScienceThe University of Nottingham Malaysia Campus Biotechnology Research Centre The University of Nottingham Malaysia Campus

Dengue has been ranked as one of the top emerging diseases in Asia and Latin *** epidemiological data may not even reflect the true burden of disease due to underreported *** control programmes have failed to contain the disease and worst of all,no specific treatment is available at the ***,this pushes the demand for a dengue vaccine as a long-term protective *** there are numerous vaccine candidates ahead,they could be held back by different aspects in promoting vaccine *** for developing nations,logistics and cost are the major hurdles that need to be addressed in order to provide a quick yet affordable medical *** an alternative,plantbased vaccine production system is able to offer an attractive prospect given to its advantages of biocontainment warranty,low operation cost,rapid scalability and logistics *** that have embarked on this scope are laid out and reviewed in terms of the feasibility of plant system to serve as a biofactory for dengue vaccine.

关键词： Dengue vaccine protective efficacy Developing countries Plant molecular pharming

protective effect of inactivated hepatitis A vaccine against the outbreak of hepatitis A in an open rural community

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World Journal of Gastroenterology 2008年第17期14卷 2771-2775页

作者： Yue-Gen Shen Xie-Jun Gu Jian-Hong Zhou Xiuzhou DistrictConter for Disease Control and Prevention Jiaxing 314001Zhejiang Province China

AIM： To evaluate the protective effect of inactivated hepatitis A vaccine （Healive） against hepatitis A outbreak in an emergency vaccination campaign. METHODS： During an outbreak of hepatitis A in Honghe Town, Xiuzhou District, Jiaxing City, Zhejiang Province, two nonrandomized controlled trials were conducted in September 2006. The first trial was to vaccinate 108 anti-HAV negative individuals with close contacts of the patients from September with 1 dose of an inactivated hepatYds A vaccine, HeaUve. The control group comprised of 115 individuals with close contacts of the patients before September. The second trial was to vaccinate 3365 primary and secondary school students who volunteered to receive a dose of Healive and 2572 students who did not receive Healive serving as its controls. An epidemiological survey was conducted to evaluate the pmtectk, e efficacy of the vaccine. RESULTS： A total of 136 hepatitis A cases were reported during an outbreak that started in June, peaked in August and September, and ended after December of 2006. After a massive vaccination of school children in September, the number of cases declined significantly. No hepatitis A was detected in the 108 vaccinated individuals with dose contacts of patients, whereas 4 cases of hepatitis A were found in the controls. The infection rate of hepatitis A was not significantly different in the individuals with close contacts of patients whether or not they received the vaccine （P = 0.122）. No hepatitis A was detected in the 3365 students who received the vaccine, four cases of hepatitis A were found in the controls. The infection rate of students with or without vaccination was significantly diffeent in the students who received the vaccine （0/3365 vs 4/2572, P = 0.035）. The protective efficacy of the vaccine was 100%.CONCLUSION： Inactivated hepatitis A vaccine demonstrates a good protective effect against an outbreak of hepatitis A.

关键词： Hepatitis A Outbreak Inactivated hepatitis Avaccine Emergency vaccination protective efficacy