Immunogenicity and protective efficacy of Vibrio harveyi pcFlaA DNA vaccine in Epinephelus awoara

作     者：覃映雪 苏永全 王世峰 鄢庆枇 

作者机构：Fisheries CollegeJimei University Department of OceanographyXiamen University Ocean College of Hainan University 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2009年第27卷第4期

页      面：769-774页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：Supported by Fujian Science and Technology Innovation Foundation for Young Scientists (No.2006F3096) Scientific Research Foundation of Jimei University 

主　　题：Vibrio harveyi DNA vaccination immunogenicity protective efficacy 

摘      要：The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1（＋） （designated as pcFlaA）. Ninety grouper （Epinephelus awoara） were separated into three equal size groups. An experimental group was immunized with pcFlaA, Control I group was immunized with the vector pcDNA3.1（＋）, and Control 1I group was immunized with PBS. The expression of pcFlaA mRNA and protein was examined using reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry. We also evaluated the immunogenicity and protective efficacy of pcFlaA against V. harveyi by measuring the lymphocyte proliferation response and serum levels of specific antibody and conducting a bacterial challenge test. We successfully transfected the fish muscle with pcFlaA. The pcFlaA mRNA and protein was expressed in the muscle cells for up to one month following injection. The proliferation response of lymphocytes in fish immunized with pcFlaA was significantly higher than in control group II. Furthermore, the immunized fish generated specific antibody. The vaccination also resulted in significantly higher survival during the bacterial challenge test.