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Protective Effect of propyl gallate Against Oxidized Low-Density Lipoprotein-Induced Injury of Endothelial Cells

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Chinese Journal of Integrative Medicine 2015年第4期21卷 299-306页

作者：马路朱晓法吴育云陈可冀史大卓殷惠军 Department of Geriatric Cardiology the Navy General Hospital of Chinese PLA The Lab of Cardiovascular Molecular Biology Xiyuan HospitalChina Academy of Chinese Medical Sciences

Objective： To evaluate the protective effect of propyl gallate （PG）, an alkyl ester of gallic acid which is an active ingredient of Radix Paeoniae, against oxidized low-density lipoprotein （ox-LDL）-induced apoptosis and death in endothelial cells （ECs） and to find out its preliminary mechanism. Methods： The cultured endothelial cells were divided into normal, model （ox-LDL）, control （fetal bovine serum）, PG high dose （20 p,g/mL）, PG middle dose （10 μg/mL）, and PG low dose （5 μg/mL） groups, each derived from three different pools of umbilical cords. The model of injured human umbilical vein endothelial cells （HUVECs） was induced by ox-LDL. The 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-2H-tetrazolium bromide （MTT） assay, Hoechst 33258 staining, flow cytometry and measurement of nitrogen monoxidum （NO） release were used to evaluate the protective effect of PG against ox-LDL-induced apoptosis and death in HUVECs. To find out the mechanism of this protective effect, the expression of endothelial nitric oxide synthase （eNOS） mRNA, eNOS protein expression, immunofluorescence of intracellular reactive oxygen species （ROS） and activities of malondialdehyde （MDA）, superoxidedismutase （SOD） and glutathione peroxidase （GPx） were observed. Results： PG significantly reduced ox-LDL-induced apoptosis and cell death. The percentage of cells death and apoptosis was significantly higher in the ox-LDL group than that in the control group （P〈0.05）. Compared with the control group, the cells death and apoptosis of PG group was no different （P〉0.05）. As compared with the ox-LDL group, results of the PG high dose group showed that cell viability was significantly increased （P〈0.05）, the level of NO release, expression of eNOS mRNA, densitometric value of eNOS protein expression, as well as the activities of SOD and GPx were all significantly higher （all P〈0.05）. Conclusion： PG could potentially serve as a novel endothelial protective agent against ox-LDL-induced injury of endothelial cell.

关键词： propyl gallate oxidized low-density lipoprotein endotheliocyte reactive oxygen species Radix Paeoniae Chinese medicine

Thermally Reversible,Self-Healing Polyurethane Based on propyl gallate and Polyurethane Prepolymers with Varied Isocyanate Content

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Journal of Renewable Materials 2020年第1期8卷 1-11页

作者： Haiyang Ding Xiaohua Yang Lina Xu Shouhai Li Jianling Xia Mei Li Institute of Chemical Industry of Forest Products(ICIFP) Nanjing210042China Key Laboratory of Biomass Energy and Material Nanjing210042China National Engineering Laboratory for Biomass Chemical Utilization Nanjing210042China Key and Open Laboratory on Forest Chemical Engineering SFANanjing210042China Co-Innovation Center of Efficient Processing and Utilization of Forest Resources Nanjing Forestry UniversityNanjing210042China

Thermosetting polyurethanes are widely used in various fields owing to their excellent elasticity,strength and solvent *** environmental friendly propyl gallate-based self-healing polyurethanes were prepared from polyurethane prepolymers with varying isocyanate *** thermal stabilities of the polyurethanes were tested using thermogravimetric *** self-healing and mechanical properties were analyzed using a universal testing machine and dynamic thermomechanical *** polyurethanes were found with high self-healing ability and excellent mechanical properties due to the absence of phenolic *** qualities improved with increased isocyanate content and the prolonged selfhealing *** found,therefore,that the propyl gallate-based polyurethane has potential for use in industrial applications as self-healing materials.

关键词： propyl gallate thermosetting polyurethane self-healing ability mechanical property

Effects of propyl gallate on Adhesion of Polymorphonuclear Leukocytes to Human Endothelial Cells Induced by Tumor Necrosis Factor Alpha

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Chinese Journal of Integrative Medicine 2009年第1期15卷 47-53页

作者：蒋跃绒陈可冀许勇钢杨晓红殷惠军 Department of Cardiovascular Diseases Xiyuan HospitalChina Academy of Chinese Medical Sciences Laboratory of Hematopathy Xiyuan HospitalChina Academy of Chinese Medical Sciences

Objective:To investigate the effects of propyl gallate(PrG) on cellular adhesion between human umbilical vein endothelial cells(HUVEC) and polymorphonuclear leukocytes(PMN) as well as the expression of intercellular adhesion molecule-1(ICAM-1,CD54) and E-selectin(CD62E) on the VEC ***: A human VEC inflammation model was induced by tumor necrosis factor alpha(TNF-α).VECs were preincubated with varying concentrations of PrG(0.001-5 mmol/L) or 1‰DMSO(v:v) or 10 mmol/L acetylsalicylic acid(ASA) for ...

关键词： cellular adhesion intercellular adhesion molecule-1 E-selectin propyl gallate

Effects of propyl gallate on Carotid Artery Thrombosis and Coagulation/Fibrinolysis System in Rats

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Chinese Journal of Integrative Medicine 2008年第1期14卷 42-45页

作者：蒋跃绒殷惠军刘剑刚马路陈可冀 Xiyuan Hospital China Academy of Chinese Medical SciencesBeijing(100091)China

Objective： To investigate the effects of propyl gallate （PrG） on the thrombus formation time and the coagulation/fibrinolysis system in an experimental carotid artery thrombosis model in rats. Methods： Fifty SD rats were randomly divided into 5 groups （10 animals/group）： the normal group （normal saline 2 mL/kg）, the model group （normal saline, 2 mL/kg）, the heparin control group （1 250 IU/kg）, the low dose PrG group （30 mg/kg）, and the high dose PrG group （60 mg/kg）. Thirty minutes after intravenous injection of saline or the corresponding drugs, a carotid artery thrombus was induced by continuous electric stimulation in all rats except for those in the normal group. The duration from the initiation of the electric stimulation to the sudden drop in carotid temperature was recorded as the thrornbus formation time. Levels of plasma tissue-type plasminogen activator （t-PA） and plasminogen activator inhibitor-1 （PAl-1） were determined by ELISA. Results： PrG （30 and 60 mg/kg） can prolong the thrombus formation time, but the effect was obviously weaker than that of heparin （P〈0.05, P〈0.01）. Compared with the model group, PrG （30 and 60 mg/kg） elevated the plasma activity of t-PA （both P〈0.05） and showed an increasing tendency in elevating the ratio of t-PA/PAI-1 （P〉0.05）, while it had no significant effect on the level of PAI-1. Conclusion： PrG has a certain antithrombotic effect and can slightly regulate the imbalance of the t-PA/PAl-1 ratio.

关键词： propyl gallate carotid artery thrombosis tissue-type plasminogen activator,plasminogen activator inhibitor

Effect of propyl gallate on Activity of Cyclooxygenase 1 and 2 in Mice's Peritoneal Macrophages*

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Chinese Journal of Integrated Traditional and Western Medicine 2004年第3期10卷 213-217页

作者：殷惠军蒋跃绒吴晓华陈晓红陈可冀 Xiyuan Hospital China Academy of Traditional Chinese Medcine Beijing (100091) China Fujian Mindong Rejuvenation Pharmaceutical Co. Ltd. Fuzhou Institute of Materia Medica Chinese Academy of Medical Sciences

Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages. Methods: A screening model for COX inhibitors in vitro based on murine peritoneal macrophages was used. COX-1 activity was reflected by the level of 6-ketoprostaglandin F1α(6-keto-PGF1α) in supernatants of cultured macrophages which were stimulated with calcium ionophore A23187 for a short-term, while COX-2 activity was reflected by the level of prostaglandin E2 (PGE2) in supernatants of cultured macrophages which were stimulated with lipopolysaccharide (LPS) for a long-term. Results: PrG did not affect A23187-induced, COX-1-derived 6-keto-PGF1α synthesis at the concentrations of 1×10-5, 5×10-6 mol/L (P>0.05), but enhanced 6-keto-PGF1α synthesis at the concentrations of 1×10-6, 5×10-7, 1×10-7 mol/L (P<0.01) in vitro, and showed a good dose-dependent manner. It inhibited LPS-induced, COX-2-derived PGE2 synthesis at the concentrations of 1×10-5,1×10-6 mol/L (P< 0. 05). Conclusion: Within the range of 1×10-5 to 1×10-7 mol/L, PrG activated COX-1 at lower concentrations and inhibited COX-2 at higher concentrations in murine peritoneal macrophages.

关键词： propyl gallate cyclooxygenase 1 cyclooxygenase 2 inflammation

Insight into synergistic antioxidation mechanisms of butyl hydroxyanisole with common synthetic antioxidants

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Grain & Oil Science and Technology 2022年第3期5卷 114-130页

作者： Jun Li Jiali Chen Yanlan Bi Huifang Yang College of Food Science and Engineering Henan University of TechnologyZhengzhou 450001China Sinograin Oils&Fats(Xinzheng)Co. Ltd.Xinzheng 451100China

Butyl hydroxyanisole(BHA) is usually blended with other synthetic antioxidants to improve the antioxidative property due to synergistic antioxidation. However, the synergistic antioxidation mechanisms of BHA with synergists have not been revealed yet. Thus, the antioxidation of BHA with butylated hydroxytoluene(BHT), tert-butylhydroquinone(TBHQ), or propyl gallate(PG) was investigated in the 2,2-azobis(2-amidino-propane) dihydrochloride oxidizing system. The contents of BHA, BHT, TBHQ,PG, and transformation products were measured by high-performance liquid chromatography. Transformation products were identified with liquid chromatography-mass spectrometry and gas chromatography–mass spectrometry. Results showed that synergistic antioxidation occurred between BHA and BHT, TBHQ, or PG. The synergistic antioxidation effect of BHA and BHT was attributed to the regeneration of BHA by BHT. Transformation products of BHA and BHT(compounds 6 and 7, dimers of BHA and BHT) had little contribution due to the relatively low content(<0.6%). The synergistic antioxidation effect of BHA and TBHQ or BHA and PG was attributed to the protective mechanism of TBHQ or PG on BHA. No transformation products were detected of BHA and TBHQ. Transformation products of BHA and PG(compounds 9 and 10, dimers of BHA and PG) had limited contribution due to the relatively low percentage(<7%). Therefore, BHA and BHT performed competitive antioxidation,while BHA and TBHQ or PG performed protective antioxidation.

关键词： Butyl hydroxyanisole Butylated hydroxytoluene tert-Butylhydroquinone propyl gallate Synergistic antioxidation

IDENTIFYING　SCANVENGING　EFFECTS　OF　ANTIOXIDANTS　ON　ACTIVE　OXYGEN　BY　A　NEW　SYSTEM

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中国科学院大学学报(中英文) 1994年第1期 78-83页

作者： DETERMINATION OF CYANIDE BY A SPECTROPHOTOMETRIC REVERSED FLOW INJECTION METHODMa Yao Zhang Jianzhong(Department of Chemistry, Graduate School, Acadeinia Sinica)Cong Jianbo Wu Ke Sun Cunpu(Beijing instilute of Radiation Medicine)Duan Shaojin(Gang Anmen

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ＩＤＥＮＴＩＦＹＩＮＧＳＣＡＮＶＥＮＧＩＮＧＥＦＦＥＣＴＳＯＦＡＮＴＩＯＸＩＤＡＮＴＳＯＮＡＣＴＩＶＥＯＸＹＧＥＮＢＹＡＮＥＷＳＹＳＴＥＭ￥ＭａＹａｏ；ＺｈａｎｇＪｉａｎｚｈｏｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＧｒａｄｕａｔｅＳｃｈｏ...

关键词： propyl gallate spin trapping antioxidants active oxygen