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Journal of Biosciences and Medicines 2015年第12期3卷 13-18页

作者： Donatini Bruno Le Blaye Isabelle Medicine Information Formation Cormontreuil France

We investigated the presence of MAP, CMV, or Epstein-Barr virus (EBV) in the saliva of patients presenting with periodontal disease, gingivitis or oral aphthosis. We also measured methylacetate in their breath and looked for the presence of PA on their tongue. All patients were prospectively enrolled into a two-year cohort study. We compared the group presenting with CD versus the group without CD. 167 patients were enrolled. 24 patients presented with CD. CMV and MAP were found concomitantly in 67% of CD patients (17 patients) whereas only 1 patient (<0.1%;p < 0.001) has these infectious agents in the comparative group. PA was rare in CD (25% versus 70.6%;p < 0.001). MA was low, especially with an empty stomach (0.59 ppm +/? 1.45 versus 5.14 ppm +/? 8.97;p < 0.001). It was concluded that the association MAP + CMV was frequently found in oral lesions of CD at an early stage. Detection of PA, MAP and CMV, as well as breath test could be easily performed and may participate to the early diagnosis of CD.

关键词： Mycobacterium avium Cytomegalovirus Crohn propionibacterium VOC

Mass Spectrometric Identification of propionibacterium Isolates Requires Database Enrichment

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Advances in Microbiology 2012年第4期2卷 497-504页

作者： Sophie Edouard Carine Couderc Didier Raoult Pierre-Edouard Fournier Unite de Recherche sur les Maladies Infectieuses Tropicales et Emergentes(URMITE) Aix-Marseille UniversiteMarseilleFrance Federation de Microbiologie Clinique Hopital de la TimoneMarseilleFrance

propionibacterium species are mostly environmental bacteria, some being commensal of mammals including humans, and sometimes pathogenic. These bacteria are poorly identified using routine laboratory methods. Recently, Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) has emerged as a rapid and efficient method to identify bacterial species. We evaluated the use of MALDI-TOF-MS for identification of all validated propionibacterium species. Only four of the 15 tested reference strains (26.7%) were correctly identified at the species level, and P. acnes, the most common human pathogenic species was not identified. When applying MALDI-TOF-MS to 48 P. acnes strains, only 18.7% were correctly identified, suggesting an intraspecific variability of proteic profiles among propionibacterium strains. However, by enriching the Bruker database with spectra from five of these strains and re-testing the other 43 strains against this new database, 93.0% were correctly identified. Our study demonstrates that MALDI-TOF-MS may be used for the identification of propionibacterium isolates but requires a database enrichment in spectra from additional isolates.

关键词： propionibacterium propionibacterium acnes MALDI-TOF Mass Spectrometry

外源有机酸对于propionibacterium freudenreichii CCTCC M207015发酵生产丙酸的影响

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食品与发酵工业 2009年第5期35卷 16-21页

作者：王桂兰冯小海吴波李莎徐虹南京工业大学制药与生命科学学院材料化学工程国家重点实验室

考察了外源有机酸(丙酸、乙酸、琥珀酸)对于游离细胞与纤维床反应器生产丙酸的影响。结果表明,以propionibacterium freudenreichii CCTCC M207015为生产菌株时,纤维床反应器具有比游离细胞更强的丙酸、乙酸耐受性,其中丙酸的抑制现象... 详细信息

考察了外源有机酸(丙酸、乙酸、琥珀酸)对于游离细胞与纤维床反应器生产丙酸的影响。结果表明,以propionibacterium freudenreichii CCTCC M207015为生产菌株时,纤维床反应器具有比游离细胞更强的丙酸、乙酸耐受性,其中丙酸的抑制现象比乙酸的更加严重。此外,外源琥珀酸可以作为丙酸合成的前体物质对*** CCTCC M207015发酵生产丙酸产生促进作用。

关键词： propionibacterium freudenreichii CCTCC M207015 游离细胞纤维床反应器琥珀酸

利用聚乙烯醇-海藻酸钙固定化propionibacterium freudenreichii NX-4制备丙酸

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南京工业大学学报（自然科学版） 2008年第4期30卷 20-24页

作者：李凯冯小海吴波章燕徐虹南京工业大学制药与生命科学学院材料化学工程国家重点实验室江苏南京210009

以propionibacterium freudenreichii NX-4为研究对象,采用聚乙烯醇(PVA)和海藻酸钠互配作为固定化载体,考察了其制备丙酸的最佳固定化条件:ρ(PVA)为10.7 g/L,ρ(海藻酸钠)为1.4 g/L,包埋量16%(质量分数),ρ(硼酸)为50 g/L,固定化时间1... 详细信息

以propionibacterium freudenreichii NX-4为研究对象,采用聚乙烯醇(PVA)和海藻酸钠互配作为固定化载体,考察了其制备丙酸的最佳固定化条件:ρ(PVA)为10.7 g/L,ρ(海藻酸钠)为1.4 g/L,包埋量16%(质量分数),ρ(硼酸)为50 g/L,固定化时间18 h.经15批次的发酵验证,产量稳定,丙酸平均产量为19.64 g/L(葡萄糖40 g/L).以80 g/L的葡萄糖为C源固定化制备丙酸192 h,丙酸产量达35.4 g/L.

关键词：固定化 propionibacterium freudenreichii NX-4 丙酸海藻酸钙聚乙烯醇

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利用纤维床反应器固定化发酵生产丙酸

生物工程学报 2008年第6期24卷 1075-1079页

作者：冯小海吴波沈晓波徐虹南京工业大学制药与生命科学学院材料化学国家重点实验室南京210009

构建了一种纤维床反应器(FBB),并将其应用于丙酸的生产。将棉纤维绕成桶状,固定于反应器中,即可用于丙酸固定化发酵。以40g/L的葡萄糖为碳源,与游离细胞相比,利用FBB生产丙酸,丙酸产量由14.58g/L提高至20.41g/L,发酵时间由120h缩短至60... 详细信息

构建了一种纤维床反应器(FBB),并将其应用于丙酸的生产。将棉纤维绕成桶状,固定于反应器中,即可用于丙酸固定化发酵。以40g/L的葡萄糖为碳源,与游离细胞相比,利用FBB生产丙酸,丙酸产量由14.58g/L提高至20.41g/L,发酵时间由120h缩短至60h。研究了不同糖浓度条件下FBB生产丙酸情况,并将补料策略应用于丙酸发酵中。结果表明:补料发酵能够有效改善propionibacterium freudenreichii CCTCC M207015在高糖条件下丙酸对葡萄糖转化率较低、副产物较多的问题。经补料发酵280h,丙酸产量达45.91g/L,丙酸质量约占有机酸总质量比例为72.31%。

关键词：纤维床反应器 propionibacterium freudenreichii CCTCC M207015 丙酸补料发酵

Intratumoural microorganism can affect the progression of hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2024年第10期16卷 4232-4243页

作者： Bao-Qun Liu Yi Bai Da-Peng Chen Ya-Min Zhang Tian-Ze Wang Jing-Rui Chen Xiang-Yu Liu Bin Zheng Zi-Lin Cui First Central Clinical College Tianjin Medical UniversityTianjin 300070China Department of Hepatobiliary Surgery Tianjin First Central HospitalSchool of MedicineNankai UniversityTianjin 300192China Tianjin First Central Hospital Clinic Institute School of MedicineNankai UniversityTianjin 300192China School of Medicine Tianjin UniversityTianjin 300072China

BACKGROUND Several recent studies have confirmed that intratumoural microorganisms can affect the occurrence and development of hepatocellular carcinoma(HCC);however,their role in tumor progression remains ***,there is a need for further research on the role of intratumoural microorganisms in *** To investigate the changes in intratumoural microorganisms in HCC and the effect of propionibacterium on HCC *** HCC and normal liver tissue specimens were subjected to fluorescence in situ hybridization(FISH).After performing 16S rRNA sequencing on HCC and peritumoral tissues to analyze the differences between the two *** was cocultured with HCC cells in *** in cell proliferation and migration capacity were *** expression of NF-κB pathway related proteins in tumor cells was *** orthotopic liver implantation model and the subcutaneous xenograft model were *** tissues and subcutaneous tumors were collected 2 weeks *** FISH demonstrated the presence of microorganisms in HCC and normal liver tissues.16S rRNA sequencing revealed an abundance of Lysobacter,Lachnospiraceae,Pseudomonas,and Lactobacillus in HCC *** distribution and abundance of propionibacterium showed differences between HCC and peritumoral tissues(Ppropionibacterium and its metabolite propionic acid(PA)inhibited the proliferation and migration of HCC cells(Ppropionibacterium in HCC exerts an effect on the NF-κB pathway,thereby affecting the biological behavior of HCC.

关键词： Hepatocellular carcinoma Intratumoural microbiome 16S rRNA sequencing propionibacterium

Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

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World Journal of Gastroenterology 2008年第8期14卷 1192-1203页

作者： Riina A Kekkonen Elina Kajasto Minja Miettinen Ville Veckman Riitta Korpela Ilkka Julkunen University of Helsinki Institute of Biomedicine Pharmacology PO Box 63 Helsinki 00014 Finland Valio Ltd Research Centre Meijeritie 4 00370 Helsinki Finland National Public Health Institute Department of Viral Diseases and Immunology Mannerheimintie 166 Helsinki 00300 Finland University of Helsinki Institute of Biomedicine Pharmacology PO Box 63 Helsinki 00014 Finland Foundation for Nutrition Research PO Box 30 Valio 00039 Finland Valio Ltd Research Centre Meijeritie 4 00370 Helsinki

AIM： To investigate the capacity of potentially probiotic strains from six bacterial genera to induce cytokine production alone or in combinations in order to identify potential enhancing or synergistic effects in order to select probiotic bacteria for in vivo purposes.METHODS： Cytokine production in human peripheral blood mononuclear cells （PBMC） in response to stimulation with eleven different potentially probiotic bacterial strains from Streptococcus, Lactobacillus, Bifidobacterium, Lactococcus, L euconostoc a n d propionibacterium genera was analysed. Production and mRNA expression of TNF-α, IL-12, IFN-y and IL-10 were determined by ELISA and Northern blotting, respectively.RESULTS： All tested bacteria induced TNF-α production. The best inducers of Thl type cytokines IL-12 and IFN-y were Streptococcus and Leuconostoc strains. All BiHdobacterium and propionibacterium strains induced higher IL-IO production than other studied bacteria. Stimulation of PBMC with any bacterial combinations did not result in enhanced cytokine production suggesting that different bacteria whether gram-positive or gram- negative compete with each other during host cell interactions.CONCLUSION： The probiotic S. thermophilus and Leuconostoc strains are more potent inducers of Thl type cytokines IL-12 and IFN-γ than the probiotic Lactobacillus strains. Bacterial combinations did not result in enhanced cytokine production.

关键词： Probiotics Streptococcus Lactobacillus Bifidobacterium lactococcus leuconostoc propionibacterium Immune response Cytokines Mononuclear cells

Detection of bacterial DNA from cholesterol gallstones by nested primers polymerase chain reaction

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World Journal of Gastroenterology 1998年第3期4卷 54-57页

作者： WU Xiao Ting 1, XIAO Lu Jia 2, LI Xing Quan 3 and LI Jie Shou 1

AIM To search for bacterial DNA sequences in cholesterol gallstones with negative bacterial culture. METHODS DNA was extracted from cholesterol gallstones in gallbladders and nested primers polymerase chain reaction (NP PCR) was used to amplify bacterial gene fragments for identifying the existence of bacteria. The samples of bacterial DNA extracted from potentially causative or unrelated living bacteria were amplified in vitro as the standard markers and comparative 16S ribosomal RNA sequence analysis was made for bacterial identification. RESULTS The gallbladder gallstones of 30 patients were analyzed and bacterial DNA was found in 26 patients. Among them, gallstones with cholesterol content between 30%-69% were seen in 5 (5/5) patients, 70%-90% in 11 (11/14) patients, and more than 90% in 10 (10/11) patients. There was no difference either in cholesterol and water content of gallstones or in harboring bacterial DNA of gallstones. *** related DNA fragments appeared in the stones of 8 (26 67%) patients; propionibacteria type DNA in 7 (23 33%); and harbored bacterial gene fragments in 2 patients, similar to Streptococcus pyogenes . A more heterogeneous sequence collection was found in 7 (23 33%) patients, which could belong to multiple bacterial infections. Two (6 67%) patients had bacterial DNA with low molecular weight which might be related to some unidentified bacteria. CONCLUSION Most cholesterol gallstones harbor bacterial DNA. It is important to determine whether these microorganisms are innocent bystanders or active participants in cholesterol gallstone formation.

关键词： cholelithiasis/microbiology propionibacterium acnes staphylococcus aureus DNA, bacterial polymerase chain reaction

Analysis of the Antimicrobial Properties of Thanaka, a Burmese Powder Used to Treat Acne

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Journal of Biosciences and Medicines 2017年第7期5卷 1-6页

作者： Elizabeth V. Seiverling Jordan P. Trubiano Jacqueline C. Williams Hadjh T. Ahrns David W. Craft Matthew R. England Departments of Dermatology Family and Community Medicine and Pathology Pennsylvania State University - Milton S. Hershey Medical Center Hershey PA USA

Thanaka, powder from Hesperethusa crenulata tree bark, has been used in Burmese culture for acne treatment and prevention for over 2000 years. The purpose of this study was to evaluate the antimicrobial properties of thanaka against Staphylococcus aureus, Escherichia coli, and propionibacterium acnes. Kirby-Bauer disk diffusion revealed no zones of inhibition for thanaka against the tested microorganisms. Disk diffusion may not be the best modality for definitive analysis of the antimicrobial activity of thanaka. Furthermore, the utility of thanaka in acne treatment may be related to anti-inflammatory, rather than antimicrobial properties.

关键词： Thanaka Myanmar Disk Diffusion propionibacterium acnes

Historical transition of management of sarcoidosis

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World Journal of Respirology 2015年第1期5卷 4-16页

作者： Minoru Inomata Satoshi Konno Arata Azuma Department of Pulmonary Medicine and Oncology Graduate School of MedicineNippon Medical School First Department of Medicine Hokkaido University School of Medicine

Sarcoidosis is a systemic granulomatous disease of undetermined etiology, and it primarily affects the lungs and lymphatic system but may involve other organs. Recently, there have been several new insights in Japanese patients. The frequency of cardiac, ocular, and cutaneous sarcoidosis has increased in Japan, whereas, the proportion of patients with bilateral hilar lymphadenopathy decreased from 1960 to *** acnes(P. acnes) has been studied extensively as one of the causative microorganism for granuloma formation, particularly in Japan. P. acnes-specific monoclonal antibodies are useful for diagnosing sarcoidosis. The potential association between smoking and sarcoidosis has been evaluated in a Japanese study, which found a higher prevalence of sarcoidosis among young smokers than that in previous reports. Recently, 18F-fluorodeoxyglucose positron-emission tomography, which permits visualization of activated inflammation, and endobronchial ultrasonography-guided transbronchial needle aspiration have been increasingly used to diagnose sarcoidosis. Cardiac sarcoidosis is found to be the main cause of death in Japan. The 2006 revised Japanese guidelines for diagnosing cardiac sarcoidosis are useful, particularly for subclinical cardiac sarcoidosis patients. Further studies and international communication and evaluation are needed to determine the causes of sarcoidosis, identify the risk factors for progressive disease, and develop new and effective treatments.

关键词： Japan propionibacterium acnes Smoking Cardiac sarcoidosis Positron-emission tomography Endobronchial ultrasonography-guided transbronchial needle aspiration