Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

作     者：Riina A Kekkonen Elina Kajasto Minja Miettinen Ville Veckman Riitta Korpela Ilkka Julkunen 

作者机构：University of Helsinki Institute of Biomedicine Pharmacology PO Box 63 Helsinki 00014 Finland Valio Ltd Research Centre Meijeritie 4 00370 Helsinki Finland National Public Health Institute Department of Viral Diseases and Immunology Mannerheimintie 166 Helsinki 00300 Finland University of Helsinki Institute of Biomedicine Pharmacology PO Box 63 Helsinki 00014 Finland Foundation for Nutrition Research PO Box 30 Valio 00039 Finland Valio Ltd Research Centre Meijeritie 4 00370 Helsinki 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2008年第14卷第8期

页      面：1192-1203页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：The Research Council for Health of the Academy of Finland the Sigrid Juselius Foundation and Valio Research Centre 

主　　题：Probiotics Streptococcus Lactobacillus Bifidobacterium lactococcus leuconostoc Propionibacterium Immune response Cytokines Mononuclear cells 

摘      要：AIM： To investigate the capacity of potentially probiotic strains from six bacterial genera to induce cytokine production alone or in combinations in order to identify potential enhancing or synergistic effects in order to select probiotic bacteria for in vivo purposes. METHODS： Cytokine production in human peripheral blood mononuclear cells （PBMC） in response to stimulation with eleven different potentially probiotic bacterial strains from Streptococcus, Lactobacillus, Bifidobacterium, Lactococcus, L euconostoc a n d Propionibacterium genera was analysed. Production and mRNA expression of TNF-α, IL-12, IFN-y and IL-10 were determined by ELISA and Northern blotting, respectively. RESULTS： All tested bacteria induced TNF-α production. The best inducers of Thl type cytokines IL-12 and IFN-y were Streptococcus and Leuconostoc strains. All BiHdobacterium and Propionibacterium strains induced higher IL-IO production than other studied bacteria. Stimulation of PBMC with any bacterial combinations did not result in enhanced cytokine production suggesting that different bacteria whether gram-positive or gram- negative compete with each other during host cell interactions. CONCLUSION： The probiotic S. thermophilus and Leuconostoc strains are more potent inducers of Thl type cytokines IL-12 and IFN-γ than the probiotic Lactobacillus strains. Bacterial combinations did not result in enhanced cytokine production.