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Isolation, Purification, and Characterization of Exopolysaccharide Produced by leuconostoc Citreum N21 from Dried Milk Cake

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Transactions of Tianjin University 2019年第2期25卷 161-168页

作者： Yanfang Yang Fang Feng Qingqing Zhou Fangkun Zhao Renpeng Du Zhijiang Zhou Ye Han School of Chemical Engineering and Technology Tianjin University

A strain with high production of exopolysaccharide(EPS) was isolated from dried milk cake(a traditional fermented food from Inner Mongolia). The strain was called N21 and later identified as leuconostoc citreum( Leu. citreum). The strain was cultured in Man-Rogosa-Sharpe medium containing 50 g/L of sucrose for 48 h at 30 °C and the EPS purified, with a yield of 24.5 g/L. An average molecular weight of 6.07 × 10~6 g/mol was determined by high-performance size-exclusion chromatography. The structure of the purified EPS was investigated through gas chromatography, ~1H and ^(13)C nuclear magnetic resonance spectroscopy, and Fourier transform infrared spectroscopy. The results demonstrated a polysaccharide composed of D-glucopyranose units in a linear chain with consecutive α(1 → 6) linkages. No branching was found in the structure of the exopolysaccharide. The purified EPS showed high water solubility and emulsibility. Based on the thermogravimetric curve, the degradation temperature of the EPS was 308.47 °C, which suggested that the dextran in the study exhibited high thermal stability. The results indicated that Leu. citreum N21 could be widely used to produce linear EPS and that the EPS has potential applications in food science and processing as a food additive.

关键词： Exopolysaccharide Dextran Characterization leuconostoc citreum Structure

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Probiotic leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

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World Journal of Gastroenterology 2008年第8期14卷 1192-1203页

作者： Riina A Kekkonen Elina Kajasto Minja Miettinen Ville Veckman Riitta Korpela Ilkka Julkunen University of Helsinki Institute of Biomedicine Pharmacology PO Box 63 Helsinki 00014 Finland Valio Ltd Research Centre Meijeritie 4 00370 Helsinki Finland National Public Health Institute Department of Viral Diseases and Immunology Mannerheimintie 166 Helsinki 00300 Finland University of Helsinki Institute of Biomedicine Pharmacology PO Box 63 Helsinki 00014 Finland Foundation for Nutrition Research PO Box 30 Valio 00039 Finland Valio Ltd Research Centre Meijeritie 4 00370 Helsinki

AIM： To investigate the capacity of potentially probiotic strains from six bacterial genera to induce cytokine production alone or in combinations in order to identify potential enhancing or synergistic effects in order to select probiotic bacteria for in vivo purposes.METHODS： Cytokine production in human peripheral blood mononuclear cells （PBMC） in response to stimulation with eleven different potentially probiotic bacterial strains from Streptococcus, Lactobacillus, Bifidobacterium, Lactococcus, L euconostoc a n d Propionibacterium genera was analysed. Production and mRNA expression of TNF-α, IL-12, IFN-y and IL-10 were determined by ELISA and Northern blotting, respectively.RESULTS： All tested bacteria induced TNF-α production. The best inducers of Thl type cytokines IL-12 and IFN-y were Streptococcus and leuconostoc strains. All BiHdobacterium and Propionibacterium strains induced higher IL-IO production than other studied bacteria. Stimulation of PBMC with any bacterial combinations did not result in enhanced cytokine production suggesting that different bacteria whether gram-positive or gram- negative compete with each other during host cell interactions.CONCLUSION： The probiotic S. thermophilus and leuconostoc strains are more potent inducers of Thl type cytokines IL-12 and IFN-γ than the probiotic Lactobacillus strains. Bacterial combinations did not result in enhanced cytokine production.

关键词： Probiotics Streptococcus Lactobacillus Bifidobacterium lactococcus leuconostoc Propionibacterium Immune response Cytokines Mononuclear cells

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