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Cromakalin pretreatment affects mitochondrial structure and function in a rat model of ischemia/reperfusion injury

Neural Regeneration Research 2008年第9期3卷 933-938页

作者： Shilei Wang Peng Wang Qingxian Chang Yu Li Yan Jiang Shiduan Wang Department of Anesthesiology Affiliated Hospital of Qingdao University Medical College Qingdao 266003 Shandong Province China

BACKGROUND：Mitochondrial structural changes and energy dysmetabolism frequently occur subsequent to cerebral *** triphosphate（ATP）-sensitive potassium channel openers exhibit protective effects on cerebral ischemia/reperfusion ***：To validate the effects of cromakalin on mitochondrial structure and function in ischemic penumbra brain tissue in a rat model of middle cerebral artery occlusion（MCAO）.DESIGN,TIME AND SETTING：The present single-factor analysis of variance,randomized,controlled,animal experiment was performed at the Institute of Brain Science,Affiliated Hospital of Qingdao University Medical College between October 2007 and March ***：Forty male,Wistar rats were randomly divided into four groups,with 10 rats per group：sham-operated,MCAO,MCAO＋ATP-sensitive potassium channel opener（cromakalin）,and MCAO＋cromakalin＋ATP-sensitive potassium channel blocking agent（glibenclamide）.METHODS：Focal cerebral ischemia/reperfusion injury was induced by MCAO in all groups except the sham-operated *** MCAO cromakalin group was administered 10 mg/kg cromakalin（i.p.） prior to MCAO *** MCAO＋cromakalin＋glibenclamide group received an injection of 10 mg/kg cromakalin（i.v.）,and subsequently an injection of 10 mg/kg cromakalin（i.p.） prior to MCAO *** OUTCOME MEASURES：At 24 hours after cerebral ischemia/reperfusion injury,cellular apoptosis was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate（dUTP） nick-end labeling *** C expression was measured by *** addition,mitochondrial swelling,membrane fluidity,membrane phospholipid and malonaldehyde（MDA） contents,as well as Na^＋-K^＋-ATPase,Ca^2 ＋-ATPase,and superoxide dismutase（SOD） activities were ***：Compared with the sham-operated group,the three ischemia groups exhibited significantly elevated mitochondrial MDA content,reduced membrane phospholipid and ATP contents,down-regulated membra

关键词： apoptosis cromakalin cytochrome C energy metabolism mitochondria potassium channel/opener

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