Cromakalin pretreatment affects mitochondrial structure and function in a rat model of ischemia/reperfusion injury

作     者：Shilei Wang Peng Wang Qingxian Chang Yu Li Yan Jiang Shiduan Wang 

作者机构：Department of Anesthesiology Affiliated Hospital of Qingdao University Medical College Qingdao 266003 Shandong Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2008年第3卷第9期

页      面：933-938页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

主　　题：apoptosis cromakalin cytochrome C energy metabolism mitochondria potassium channel/opener 

摘      要：BACKGROUND：Mitochondrial structural changes and energy dysmetabolism frequently occur subsequent to cerebral *** triphosphate（ATP）-sensitive potassium channel openers exhibit protective effects on cerebral ischemia/reperfusion ***：To validate the effects of cromakalin on mitochondrial structure and function in ischemic penumbra brain tissue in a rat model of middle cerebral artery occlusion（MCAO）.DESIGN,TIME AND SETTING：The present single-factor analysis of variance,randomized,controlled,animal experiment was performed at the Institute of Brain Science,Affiliated Hospital of Qingdao University Medical College between October 2007 and March ***：Forty male,Wistar rats were randomly divided into four groups,with 10 rats per group：sham-operated,MCAO,MCAO＋ATP-sensitive potassium channel opener（cromakalin）,and MCAO＋cromakalin＋ATP-sensitive potassium channel blocking agent（glibenclamide）.METHODS：Focal cerebral ischemia/reperfusion injury was induced by MCAO in all groups except the sham-operated *** MCAO cromakalin group was administered 10 mg/kg cromakalin（i.p.） prior to MCAO *** MCAO＋cromakalin＋glibenclamide group received an injection of 10 mg/kg cromakalin（i.v.）,and subsequently an injection of 10 mg/kg cromakalin（i.p.） prior to MCAO *** OUTCOME MEASURES：At 24 hours after cerebral ischemia/reperfusion injury,cellular apoptosis was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate（dUTP） nick-end labeling *** C expression was measured by *** addition,mitochondrial swelling,membrane fluidity,membrane phospholipid and malonaldehyde（MDA） contents,as well as Na^＋-K^＋-ATPase,Ca^2 ＋-ATPase,and superoxide dismutase（SOD） activities were ***：Compared with the sham-operated group,the three ischemia groups exhibited significantly elevated mitochondrial MDA content,reduced membrane phospholipid and ATP contents,down-regulated membra