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Neural Regeneration Research 2023年第10期18卷 2156-2160页

作者： María A.Pajares Elena Hernández-Gerez Milos Pekny Dolores Pérez-Sala Department of Structural and Chemical Biology Centro de Investigaciones Biológicas Margarita SalasMadridSpain Laboratory of Astrocyte Biology and CNS Regeneration Center for Brain RepairDepartment of Clinical NeuroscienceInstitute of Neuroscience and PhysiologySahlgrenska Academy at the University of GothenburgGothenburgSweden University of Newcastle NewcastleNSWand the Florey Institute of Neuroscience and Mental HealthParkvilleVICAustralia

Alexander disease is a rare neurodegenerative disorder caused by mutations in the glial fibrillary acidic protein,a type III intermediate filament protein expressed in astrocytes.Both early(infantile or juvenile)and adult onsets of the disease are known and,in both cases,astrocytes present characteristic aggregates,named Rosenthal fibers.Mutations are spread along the glial fibrillary acidic protein sequence disrupting the typical filament network in a dominant manner.Although the presence of aggregates suggests a proteostasis problem of the mutant forms,this behavior is also observed when the expression of wild-type glial fibrillary acidic protein is increased.Additionally,several isoforms of glial fibrillary acidic protein have been described to date,while the impact of the mutations on their expression and proportion has not been exhaustively studied.Moreover,the posttranslational modification patterns and/or the protein-protein interaction networks of the glial fibrillary acidic protein mutants may be altered,leading to functional changes that may modify the morphology,positioning,and/or the function of several organelles,in turn,impairing astrocyte normal function and subsequently affecting neurons.In particular,mitochondrial function,redox balance and susceptibility to oxidative stress may contribute to the derangement of glial fibrillary acidic protein mutant-expressing astrocytes.To study the disease and to develop putative therapeutic strategies,several experimental models have been developed,a collection that is in constant growth.The fact that most cases of Alexander disease can be related to glial fibrillary acidic protein mutations,together with the availability of new and more relevant experimental models,holds promise for the design and assay of novel therapeutic strategies.

关键词： astrocytes endoplasmic reticulum stress glial fibrillary acidic protein mutants metabolism misassembly misfolding neurodegeneration oxidative stress posttranslational modifications unfolded protein response

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Pan-Specific Antibodies as Novel Tools to Detect Valyllysine

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Reproductive and Developmental Medicine 2020年第2期4卷 78-83页

作者： Ya-Ling Wang Min-Yan Liu Yu-Hua Li Yi-TingYang Wei-Wei Wang He-GuoYu Zhi-Yu Shao Hua Diao Department of Reproductive Biology NHC Key Laboratory of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research)School of Basic Medical SciencesFudan UniversityShanghai 200032China College of Chemistry Chemical Engineering and BiotechnologyDonghua UniversityShanghai 201620China

Objective:Amino acyl modification of lysine residues is an essential mechanism of nutrient sensing that regulates various biological functions including reproduction.At present,the lack of pan-specific antibodies for a recently identified lysine valylation hinders the characterization and detection of this modification.The objective of this study is to raise pan-specific antibodies that may facilitate the identification of novel expression patterns of lysine valylation.Methods:Chicken ovalbumin was valylated as an immunogen to raise polyclonal antibodies(PcAbs)in rabbits.The population of the pan-specific antibodies recognizing valylated lysine was purified using the chemically synthesized valylated peptides consisting of random amino acids.The specificity of the antibodies was evaluated using ELISA,dot blots,Western blots,and immunohistochemistry(IHC)staining in human epididymis as well.Results:A preliminary and simple strategy to make an anti-valylated lysine PcAb was developed.The recognition of the antibodies to valyllysine was evaluated as pan specific.This was useful for the detection of the newly identified valyl modification using ELISA,dot blots,and Western blots.The antibodies were also successfully utilized in IHC assays,which revealed novel valyllysine modification patterns in epididymis tissues of human.Conclusions:A new antibody tool was provided for the study of lysine valylation.The novel expression patterns of valyllysine in the epididymis suggest that this modification may be involved in sperm maturation.

关键词： Anti-Valyllysine Antibody Epididymis posttranslational modifications Valyllysine

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The complexity of nitric oxide generation and function in plants

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BIOCELL 2016年第1期40卷 1-5页

作者：无 MARIA P BENAVIDES SUSANA M GALLEGO FACUNDO RAMOS ARTUSO MARIANA CHECOVICH ANDREA GALATRO Instituto de Bioquímica y Medicina Molecular(IBIMOL) Universidad de Buenos Aires-CONICETBuenos AiresArgentina Cátedra Química Biológica Vegetal Instituto de Química y Fisicoquímica Biológicas"Prof.Alejandro C.Paladini"(IQUIFIB)UBA-CONICETBuenos AiresArgentina Instituto de Fisiología Vegetal(INFIVE)CONICET-UNLP La PlataArgentina Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús(IIB-INTECH) CONICET-UNSAMChascomúsArgentina

Plants are exposed to environmental stress,in natural and agricultural conditions.Nitric oxide(NO),a small gaseous molecule which plays important roles in plants,has been involved in many physiological processes,and emerged as an important endogenous signaling molecule in the adaptation of plants to biotic and abiotic stress.NO is produced from a variety of enzymatic and non enzymatic sources,which are not yet fully understood.Also,NO and reactive nitrogen species(RNS)can produce posttranslational modifications affecting protein function.Nitrate reductase,a key enzyme in the nitrogen metabolism,is a proposed source of NO in plants which could be affected by posttranslational modifications.Thus,different pathways seem to be involved and can also regulate NO synthesis in the plant cell under physiological or stress conditions.However,how the levels of NO are reached in such time and place to fulfill its functions,are still puzzles to elucidate.

关键词： nitrate reductase polyamines posttranslational modifications

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Multifunctions of Histone H1 Proteins

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Wuhan University Journal of Natural Sciences 2014年第1期19卷 8-18页

作者： WANG Wenjun CAI Ruiqi XIAO Hongdou ZHENG Ling College of Life Sciences Wuhan University Diabetes Center Wuhan University

Among various histones, histone H1 proteins have been appreciated for their multiple functions in diverse biological processes. In addition to being a structural protein in chromatin, H1 proteins also play critical roles in cell cycle, gene expression, and development. Recent studies reveal the possible effects of H1 in some diseases, such as cancer and neurodegenerative diseases. Here, we review different variants of HI, the functions, and post translational modifications of ill variants are also discussed.

关键词： histone H1 proteins posttranslational modifications gene transcription cell cycle

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Regulatory mechanism of aflatoxin AFB1 biosynthesis in Aspergillus flavus

Regulatory mechanism of aflatoxin AFB1 biosynthesis in Asper...

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多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： Guang Yang Feng Zhang Xinyi Nie Zhenhong Zhuang Shihua Wang Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province School of Life Sciences Fujian Agriculture and Forestry University

Aspergillus flavus is a saprophytic and pathogenic fungus which contaminates a variety of economical crops（such as peanuts and maize） with mycotoxins, causing huge economic losses. Aflatoxins, which are mainly produced by A. flavus and A. parasiticus, are found to be one of the most toxic and carcinogenic secondary matabolites, posing a serious threat to animals and humans. In order to eliminate the biological hazards caused by A. flavus and aflatoxin, a series factors affecting the development of A. flavus and biosynthesis of aflatoxin have been investigated in our studies. In our previous studies of transcriptome and protemics, some environment factors such as temperature, water activity and nutritions were considered to be involved in the regulation of development and aflatoxin biosynthesis in A. flavus. In addition, epigenetic and posttranslational modifications including methylation, acetylation, phosphorylation, sumoylation and succinylation have been proved to participate in multiple cellular processes in our studies. Overall, our findings not only reveal potential mechanisms of development, aflatoxin production and pathogenicity in A. flavus, but also established theoretical basis for the further research in this fungal pathogen.

关键词： Aspergillus flavus Aflatoxin Environment factors Epigenetic posttranslational modifications

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