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Chloroplast DNA reveals genetic population structure in Sinomenium acutum in subtropical China

Chinese Herbal Medicines 2023年第2期15卷 278-283页

作者： Chun Guo Ying He Xiyao Zeng Xingyao Xiong Ping Qiu Xueshuang Huang Hua Yang Center for Medical Research and Innovation The First Hospital of Hunan University of Chinese MedicineChangsha 410007China College of Bioscience and Biotechnology Hunan Agricultural UniversityChangsha 410125China Hunan Zhengqing Pharmaceutical Group Co. Ltd.Huaihua 418000China College of Horticulture and Landscape Hunan Agricultural UniversityChangsha 410125China Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine Hunan University of MedicineHuaihua 418000China

Objective:The population density and diversity of Sinomenium acutum(Menispermaceae)have been greatly reduced recently by overharvesting for medicinal purposes in ***,it is urgent that the remaining populations are investigated,and that strategies for the utilization and conservation of this species are *** study aimed to find the possible glacial refugia and define the genetic diversity of *** for its proper utilization and ***:A total of 77 *** samples were collected from four locations,Qinling Mountains,Daba Mountains,Dalou Mountains,and Xuefeng Mountains,in subtropical *** diversity among and between these populations were phylogenetically analyzed using four chloroplast DNA molecular markers(atpI-atpH,trnQ-50 rps16,trnH-psbA and trnL-trnF).Results:A total of 14 haplotypes(C1 to C14)were found in collected *** C1 and C3 were shared among all populations,with C3 as the ancestral *** C11 and C12 diverged the most from C3 and other *** obvious phylogeographic structure was found in four locations using the GST/NST *** is no evidence of rapid demographic expansion in *** based on the mismatch distribution,and the results of Tajima’s D test,and Fu’s FS *** analyses of molecular variance revealed a high level of genetic variation within *** contrast,the genetic differentiation among *** populations was low,indicating frequent gene ***:Xuefeng,Dalou,and Daba Mountains were possible glacial refugia for the populations of ***.C1,C3,C11 and C12 haplotypes of *** should be carefully preserved and managed for their genetic value.

关键词： chloroplast DNA genetic diversity phylogeny population variation Sinomenium acutum(Thunb.)Rehd.et Wils

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Thin-Plate Spline Analysis of Diversity in Field populations of Codling Moth, Cydia pomonella L. （Lep., Tortricidae） in North West of Iran

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Journal of Agricultural Science and Technology 2011年第1期5卷 35-41页

作者： S. Khaghaninia S. A. Mohammadi A. M. Sarafrazi K. Haddad Irani Nejad R. Zahiri Department of Plant Protection Faculty of Agriculture University of Tabriz Tabriz 51664 Iran Department of Agronomy ＆ Plant Breeding Faculty of Agriculture University of Tabriz Tabriz 51664 lran Institute of Plant Pests ＆ Diseases Research Tehran 19395 Iran Department of Biology University of Turku Turku 20014 Finland

In order to study morphological diversity of codling moth, Cydia pomonella （L.） using thin-plate spline analysis, nine geographical populations from four north western provinces of Iran namely East Azarbayjan, West Azarbayjan, Ardebil and Zandjan were collected during 2003 and 2004. 575 and 564 images were prepared from fore and hind wings, respectively. Then 15 and 11 landmarks were determined from fore and hind wings, respectively. With transforming of landmark＇s two dimensional coordinate data into partial warp scores, 26 and 18 scores were generated for fore and hind wings, respectively. Cluster analysis based on wing shape variables using Ward＇s algorithm assigned nine geographical populations into two groups. The pattern of grouping based on fore and hind wings was different in both sexes. Principal component analysis revealed discrimination between geographic populations and confirmed the result of cluster analysis. Among environmental parameters, wind speed showed the highest correlation with wing shape variables. Non significant correlation was observed between geographic and morphological distance matrices as revealed by Mantel test.

关键词： Geometric morphometrics population variation thin-plate spline analysis codling moth Cydia pomonella (L.)

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