Chloroplast DNA reveals genetic population structure in Sinomenium acutum in subtropical China

作     者：Chun Guo Ying He Xiyao Zeng Xingyao Xiong Ping Qiu Xueshuang Huang Hua Yang Chun Guo;Ying He;Xiyao Zeng;Xingyao Xiong;Ping Qiu;Xueshuang Huang;Hua Yang

作者机构：Center for Medical Research and InnovationThe First Hospital of Hunan University of Chinese MedicineChangsha 410007China College of Bioscience and BiotechnologyHunan Agricultural UniversityChangsha 410125China Hunan Zhengqing Pharmaceutical Group Co.Ltd.Huaihua 418000China College of Horticulture and LandscapeHunan Agricultural UniversityChangsha 410125China Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese MedicineHunan University of MedicineHuaihua 418000China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2023年第15卷第2期

页      面：278-283页

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：supported by the Hunan Provincial Major Science and Technology Project(2015SK1001) Changsha Science and Technology Plan(kq1801015) Project of Hunan Administration of Traditional Chinese Medicine(2021212) Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine(HCSW2020-01) 

主　　题：chloroplast DNA genetic diversity phylogeny population variation Sinomenium acutum(Thunb.)Rehd.et Wils 

摘      要：Objective:The population density and diversity of Sinomenium acutum(Menispermaceae)have been greatly reduced recently by overharvesting for medicinal purposes in ***,it is urgent that the remaining populations are investigated,and that strategies for the utilization and conservation of this species are *** study aimed to find the possible glacial refugia and define the genetic diversity of *** for its proper utilization and ***:A total of 77 *** samples were collected from four locations,Qinling Mountains,Daba Mountains,Dalou Mountains,and Xuefeng Mountains,in subtropical *** diversity among and between these populations were phylogenetically analyzed using four chloroplast DNA molecular markers(atpI-atpH,trnQ-50 rps16,trnH-psbA and trnL-trnF).Results:A total of 14 haplotypes(C1 to C14)were found in collected *** C1 and C3 were shared among all populations,with C3 as the ancestral *** C11 and C12 diverged the most from C3 and other *** obvious phylogeographic structure was found in four locations using the GST/NST *** is no evidence of rapid demographic expansion in *** based on the mismatch distribution,and the results of Tajima’s D test,and Fu’s FS *** analyses of molecular variance revealed a high level of genetic variation within *** contrast,the genetic differentiation among *** populations was low,indicating frequent gene ***:Xuefeng,Dalou,and Daba Mountains were possible glacial refugia for the populations of ***.C1,C3,C11 and C12 haplotypes of *** should be carefully preserved and managed for their genetic value.