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骨桥蛋白中多肽片段的FT-ICR-MS分析

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分析测试学报 2008年第S1期27卷 50-52页

作者：石磊王韶刘晓梅程舸于雷段芳芳韩丽华吉林大学公共卫生学院吉林长春130021 上海市肿瘤研究所流行病学研究室上海200032

Biological mass spectrometry has received a great progress in recent *** osteopontin(OPN) isolated and purified from the human milk was studied by HPLC using SCX and C4 *** hydrolyzing the purified OPN sample by trypsin,the correlative polypeptide fragments GDSVVYGLR and QNLLAPQTLPSK were obtained by *** showed that the method of nano-spray HPLC combined with FT-ICR-MS and Mascot search was very efficient for the analysis of the purified OPN and its polypeptide *** study provides more academic theories of their different modifications and the relative bioactivities.

关键词： osteopontin(OPN) Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR-MS) polypeptide fragments

EFFECTS OF MOLECULAR WEIGHT ON THERMAL RESPONSIVE PROPERTY OF PEGYLATED POLY-L-GLUTAMATES

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Chinese Journal of Polymer Science 2013年第2期31卷 201-201,202-210页

作者： Shusheng Zhang Chongyi Chen 李志波 Laboratory of Polymer Physics and Chemistry Institute of Chemistry Chinese Academy of Sciences

We investigated the ring opening polymerization （ROP） of di- and tri-ethylene glycol monomethyl ether functionalized L-glutamate N-carboxyanhydrides （NCAs） using hexamethyldisilazane （HMDS） as primary initiator and 1,5,7-triazabicyclo-[4.4.0]dec-5-ene （TBD） as co-initiator. The binary initiator system afforded a living ROP for these pegylated NCAs, and a series of homopolypeptides with controlled molecular weight （MW） and low polydispersity were obtained. We then systematically studied the helical content and clouding point （CP） dependence on polypeptide MW using circular dichroism （CD） spectroscopy and turbidity measurements, respectively. We found that the helical content of both homopolypeptides increased with MW, but the triethylene glycol functionalized poly-L-glutamate （poly-L-EG3Glu） intended to form more stable or-helical structure than diethylene glycol functionalized counterpart （poly-L-EG2Glu） at similar MW. Accordingly, the CP of poly-L-EG2Glu with known end group has strong dependence on its helical content, which is essentially determined by MW. Our results suggested that the thermal responsive properties of these unique pegylated poly- L-glutamates not only rely on their chemical structure but also on their secondary structures, wh^ch is different from conventional thermal responsive polymers.

关键词： N-carboxyanhydride (NCA) Ring opening polymerization polypeptide Helicity Thermal responsive.

POLYMERIZATION OF γ-STEARYL-α,l-GLUTAMATE N-CARBOXYANHYDRIDE USING RARE EARTH COORDINATION CATALYSTS

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Chinese Journal of Polymer Science 2000年第4期18卷 369-372页

作者： Xiao-ping Hu Jian Wu Zhi-kang Xu Lin-xian Feng Institute of Polymer Science, Zhejiang University, Hangzhou 310027, China Department of Chemistry, Zhejiang University, Hangzhou 310027, China 浙江大学浙江杭州

A rare earth coordination system was first investigated as a new type of catalyst for the ring-openingpolymerization of α-amino acid N-carboxyanhydrides (NCAs). The results for the polymerization of γ-stearyl-α,L-glutamate(SLG) NCA using neodymium acetylacetonate (Nd(acac)_3)- or neodymium tris(2-ethylhexylphosphonate) (Nd(P_(204))_3)-triethylaluminum-water as catalysts were compared with those using conventional catalysts. It was found that the helicalpoly(γ-stearyl-L-glutamate) with high molecular weight as well as narrow molecular weight distribution can be obtained inthe presence of Nd(acac)_3/AlEt_3-1/2H_2O. The polymer obtained was characterized by IR and NMR spectroscopy.

关键词： Rare earth coordination catalyst Ring-opening polymerization polypeptide Poly(γ-stearyl-α,L-glutamate)

Thermal and Redox Dual Responsive Poly(L-glutamate)with Oligo(ethylene glycol)Side-chains

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Chinese Journal of Polymer Science 2016年第12期34卷 1436-1447页

作者： Xiao-hui Fu Yi-nan Ma 孙静李志波 School of Polymer Science and Engineering Qingdao University of Science and Technology Laboratory of Polymer Physics and Chemistry Institute of Chemistry Chinese Academy of Sciences

A series of poly（L-glutamate）s grafted with oligo（ethylene glycol）（OEG） side-chains through the thioether linkages （PALGn-g-EGx, x = 2, 3 and 4） were prepared by ring-opening polymerization （ROP） of γ-allyl-L-glutamate N-carboxyanhydride （ALG-NCA） and thiol-ene photoaddition. The chemical structures and physical properties were characterized by 1H-NMR, Fourier transform infrared （FTIR）, circular dichroism （CD）, etc. The PALGn-g-EGx samples with x = 3 and 4 displayed lower critical solution temperature （LCST） in water due to the presence of OEG units. The clouding point （CP） of polypeptides can be finely tuned by changing the side chain structures, molecular weights and sample concentrations. In addition, the thioether linkages in the side chains offer additional redox-responsive properties. The influence of both OEG units and thioether linkages on the LCST behavior was systematically investigated. This work provides an efficient way to prepare multi-stimuli responsive materials with highly tunable properties.

关键词： N-carboxyanhydride (NCA) polypeptide Ring-opening polymerization Click chemistry Stimuli-responsive.

ELECTROSPRAYING/ELECTROSPINNING OF POLY(γ-STEARYL-LGLUTAMATE):FORMATION OF SURFACES WITH SUPERHYDROPHOBICITY

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Chinese Journal of Polymer Science 2009年第1期27卷 115-120页

作者：吴健徐志康 Department of Chemistry Zhejiang University Institute of Polymer Science and Key Laboratory of Macromolecular Synthesis and Functionalization (Ministry of Education)Zhejiang University

Electrospraying/electrospinning of poly(γ-stearyl-L-glutamate) (PSLG) was investigated on a series solutions with different concentrations in *** emission scanning electron microscopy (FESEM) and attenuated Iotal reflectance Fourier transform infrared spectroscopy (FT-IR/ATR) were used to characterize the morphology and structure of the electrosprayed/electrospun polypeptide *** was found that electrospraying of PSLG with concentrations lower than 16 wt% afforded beads,while microfibers cou...

关键词： Electrospinning/electrospraying Poly(γ-stearyl-L-glutamate) polypeptide Water contact angle Superhydrophobic surface

A novel plant defensin from Chinese mistletoe,Viscum coloratum (Kom.) Nakai

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Chinese Chemical Letters 2007年第1期18卷 55-58页

作者： Shi Lei Liu Xiu Bao Du Jing Lin Kong Hui Jiang Research Institute of Chemical Defense Beijing 102205 China

A novel polypeptide named as defensin CM was isolated from Chinese mistletoe, Viscum coloratura （Kom.） Nakai. The amino acid sequence was determined by the combination of Edman degradation, endoproteinase Lys-C digestion, and MALDI-TOF mass spectrometry. The primary structure was ATCSAPSGRF KGACFSSNTC SNICKTLEGL KDGHCTGLAC YCSRNC.

关键词： Viscum coloratura Mistletoe Primary structure Plant defensin polypeptide

Vacuum-tuned-atmosphere induced assembly of Au@Ag core/shell nanocubes into multi-dimensional superstructures and the ultrasensitive IAPP proteins SERS detection

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Nano Research 2019年第6期12卷 1375-1379页

作者： Meng Xu Guopeng Tu Muwei Ji Xiaodong Wan Jiajia Liu Jia Liu Hongpan Rong Yanlian Yang Chen Wang Jiatao Zhang Beijing Key Laboratory of Construction-Tailorable Advanced Functional Materials and Green Applications Experim ental Center of Advanced Materials School of Materials Science & EngineeringBeijing Institute of TechnologyBeijing 100081China Institute of Low-dimensional Materials Genome Initiative College of chemistry and environmental engineeringShenzhen UniversityGuangdong Shenzhen 518060China Graduate School at Shenzhen Tsinghua UniversityShenzhen 518055China National Center for Nanoscience and Technology (NCNST) Chinese Academy of SciencesBeijing 100190China

Utilizing vacuum-tuned-atmosphere induced dip coating method,we achieve the cross-dimensional macroscopic diverse self-assemblies by using one building block with one chemical *** modulating the vacuum degree,colloid concentration and evaporation atmosphere,Au@Ag core/shell nanocubes (NCs) can controllably assemble into diverse multi-dimensional *** 0.08 MPa,we obtained the two-dimensional (2D) stepped superstructures with continuously tunable step *** addition,we generated a series of tailorable nanoscale-roughened 2D Au@Ag NCs superstructures at 0.04 MPa,which exhibited the label-free ultrasensitive SERS detection for the different mutants of IAPP8-37 *** 0.01 MPa,we obtained the cross-dimensional tailorable Au@Ag NCs assemblies from random to macroscale 2D and three-dimensional (3D) densest superstructures by adjusting the capping ligand-environmental molecule *** is a flexible method to generate as-prepared Au@Ag core/shell NCs into well-defined macroscopic diverse superstructures and to promote the exploitation into biological applications.

关键词： superstructure Au@Ag core/shell nanocubes (NCs) self-assembly surface enhanced Raman scattering (SERS) detection islet arnyloid-like polypeptide

Nitric Oxide-Releasing Poly(L-glutamic acid)Hybrid Hydrogels for Accelerating Diabetic Wound Healing

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Chinese Journal of Chemistry 2023年第17期41卷 2103-2112页

作者： Lin Teng Yingying Song Lingli Hu Qian Bai Xueliang Zhang Chang-Ming Dong Schoolof Chemistry and Chemical Engineering Frontiers Science Centerfor Transformative MoleculesShanghai Key Laboratory o Electrical Insulation and Thermal AgingShanghai Jiao Tong UniversityShanghai 200240China Department of Environmental Health Schoolof Public HealthShanxi Medical UniversityTaiyuanShanxi 030012China

Comprehensive Summary,Diabetic wound healing is threatening worldwide and challenging in wound *** to inhibit heavy inflammation and bacterial infection in diabetic wounds,herein,we facilely construct a kind of NO-releasing poly(L-glutamic acid)(PGA)based graphene oxide(GO)hybrid hydrogel of HDGS at a lower solid percentage,presenting large microporous size of about 20μm,mild photothermal conversion property,and good *** improving hemostasis performance,the less amount of GO endowed the hydrogel with near infrared(NIR)responsivity to control fast and pulsatile NO release for killing bacteria and inhibiting heavy inflammation to proheal diabetic wound,in which a broad spectrum of antibacterial activities toward killing ***,*** and MRSA was achieved via a combined effect of photothermia and NO *** vivo effective hemostasis was attained in a rat liver bleeding model with short hemostatic time of~20 s and lower blood loss of 1.5%—2.0%.Moreover,the treatment of HDGS plus 4 times of mild NIR irradiation(10 min,808 nm,1 W/cm^(2)per time)performed superior full diabetic wound healing within 11—14 d,in which the regenerated skins were characteristic of thick epidermis/dermis,dense blood vessels,some hair follicles-embedding,and high level of collagens.

关键词： polypeptide Hydrogel Nitric oxide Diabetic wound Wound healing Wound dressings

Isolation and partial molecular characterization of heat-stable growth factor from human placenta

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Science China(Life Sciences) 1998年第6期41卷 628-635页

作者：方云南王希成罗利群周海梦 Department of Biological Science and Biotechnology Tsinghua University Beijing China State Key Laboratory of Biomembrane and Biomembrane Engineering Tsinghua University Beijing China

A heat\|stable growth factor has been purified to homogeneity from human placenta. Purification procedure involved extraction with acidic medium, precipitation using ethanol, DE\|52 ion\|exchange chromatography, and reversed\|phase HPLC. The purified preparation has been named human placental growth factor\|1 (HPGF\|1), which simulates the proliferation of some cell lines, such as human amniotic cell, mouse marrow tumor cell, and mouse fibroblast cell. The growth factor has an approximate molecular weight of 1 900. It is a glycopeptide which contains a polypeptide chain consisting of 10 amino acid residues with molecular weight of 1 195. The N\|terminal residue of the polypeptide chain part is phenylalanine. The molecular weight of non\|peptide part is 682. The isoelectric point of the growth factor is pI 6.8.

关键词： human placenta growth factor active polypeptide purification.