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Involvement of polyamine oxidase in Abscisic Acidinduced Cytosolic Antioxidant Defense in Leaves of Maize

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Journal of Integrative Plant Biology 2009年第3期51卷 225-234页

作者： Beibei Xue Aying Zhang Mingyi Jiang College of Life Sciences Nanjing Agricultural University Nanjing 210095 China

Using pharmacological and biochemical approaches, the role of maize polyamine oxidase （MPAO） in abscisic acid （ABA）- induced antioxidant defense in leaves of maize （Zea mays L.） plants was investigated. Exogenous ABA treatment enhanced the expression of the MPAO gene and the activities of apoplastic MPAO. Pretreatment with two different inhibitors for apoplastic MPAO partly reduced hydrogen peroxide （H202） accumulation induced by ABA and blocked the ABA-induced expression of the antioxidant genes superoxide dismutase 4 and cytosolic ascorbate peroxidase and the activities of the cytosolic antioxidant enzymes. Treatment with spermidine, the optimum substrate of MPAO, also induced the expression and the activities of the antioxidant enzymes, and the upregulation of the antioxidant enzymes was prevented by two inhibitors of MPAO and two scavengers of H202. These results suggest that MPAO contributes to ABA-induced cytosolic antioxidant defense through H202, a Spd catabolic product.

关键词： abscisic acid cytosolic antioxidant defense hydrogen peroxide polyamine oxidase Zea mays.

Regulatory Function of polyamine oxidase-Generated Hydrogen Peroxide in Ethylene-Induced Stomatal Closure in Arabidopsis thaliana

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Journal of Integrative Agriculture 2013年第2期12卷 251-262页

作者： HOU Zhi-hui LIU Guo-hua HOU Li-xia WANG Lan-xiang LIU Xin College of Life Sciences Qingdao Agricultural University/University Key Laboratory of Plant Biotechnology in Shandong Province Universities of Shandong

Hydrogen peroxide （H2O2） is an important signaling molecule in ethylene-induced stomatal closure in Arabidopsis thaliana. Early studies on the sources of H2O2 mainly focused on NADPH oxidases and cell-wall peroxidases. Here, we report the involvement of polyamine oxidases （PAOs） in ethylene-induced H2O2 production in guard cells. In Arabidopsis epidermal peels, application of PAO inhibitors caused the failure of ethylene to induce H2O2 production and stomatal closure. Results of quantitative RT-PCR analysis and pharmacological experiments showed that AtPAO2 and AtPAO4 transcripts and activities of PAOs were both induced by ethylene. In transgenic Arabidopsis plants over-expressing AtPAO2 and AtPAO4, stomatal movement was more sensitive to ethylene treatment and H2O2 production was also significantly induced. The increased H2O2 production in the transgenic lines compared to the wild-type plants suggests that AtPAO2 and AtPAO4 probably are involved in ethylene-induced H2O2 production. Several factors which induce stomatal closure such as dehydration and high salinity all enhanced the expression of AtPAO2 and AtPAO4 to different degrees. Moreover, GFP- AtPAOs fusion protein localized in the nucleus, cytoplasm, and cell wall of the guard cells. Therefore, our results strongly indicated that PAO is a source of H2O2 generation in Arabidopsis guard cells and plays crucial roles in stomatal movement.

关键词： Arabidopsis thaliana ethylene guard cell hydrogen peroxide polyamine oxidase

Thymol ameliorates ammonium toxicity via repressing polyamine oxidase-derived hydrogen peroxide and modulating ammonium transporters in rice root

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Food Production, Processing and Nutrition 2021年第1期3卷 106-114页

作者： Kai Guo Guangchi An Ning Wang Bingdong Pang Zhiqi Shi Hongwu Bai Li Zhang Jian Chen Weimin Xu National Tobacco Cultivation and Physiology and Biochemistry Research Center/Key Laboratory for Tobacco Cultivation of Tobacco Industry Henan Agricultural UniversityZhengzhou 450002China Biology Institute Qilu University of Technology(Shandong Academy of Sciences)Shandong Province Key Laboratory for BiosensorsJinan 250014China Zaozhuang Technology Promotion Center of Agriculture and Agricultural Machinery Zaozhuang 277800China Central Laboratory Jiangsu Academy of Agricultural SciencesNanjing 210014China Institute of Food Safety and Nutrition Jiangsu Academy of Agricultural Sciences/Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and TechnologyNanjing 210014China College of Plant Protection Shandong Agricultural UniversityTaian 271018China Supervision&Testing Center for Processed Agri-products(Nanjing) Ministry of AgricultureNanjing 210014China

Background:Ammonium is an indispensable nutrient for crop growth,but anoxic conditions or inappropriate fertilizer usage result in the increase in ammonium content in *** ammonium causes *** is a kind of natural phenolic compound with anti-microbial ***,little is known about the role of thymol in modulating plant *** we find the novel role of thymol in protecting rice from ammonium ***:Thymol remarkably rescued rice seedlings growth from ammonium stress,which may resulted from the attenuation of reactive oxygen species(ROS)accumulation,oxidative injury,and cell death in both shoots and *** oxidase(PAO)metabolizes polyamines to produce ROS in plants in response to stress *** blocked ammonium-induced upregulation of a set of rice PAOs,which contributed to the decrease in ROS *** rice seedlings upon ammonium stress,thymol downregulate the expression of ammonium transporters(AMT1;1 and AMT1;2);thymol upregulated the expression of calcineurin B-like interacting protein kinase 23(CIPK23)and calcineurin B-like binding protein 1(CBL1),two negative regulators of *** may help rice avoid ammonium overload in excessive ammonium *** analysis indicated that PAOs,AMTs,and CBL1 were the targets of thymol in the detoxification of excessive ***:Thymol facilitates rice tolerance against ammonium toxicity by decreasing PAO-derived ROS and modulating ammonium *** findings may be applicable in the modulation of nutrient acquisition during crop production.

关键词： Ammonium toxicity polyamine oxidase Reactive oxygen species Rice Thymol

Genome-wide analysis of the polyamine oxidase gene family in Citrus sinensis

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Genome-wide analysis of the polyamine oxidase gene family in...

The Improvement of Soybean Salt Tolerance by Overexpressed GmPAO1

从植物科学到农业发展——2012全国植物生物学大会

作者： Wei Wang,Jihong Liu College of Horticulture & Foresty Science,Huazhong Agricultural University,Wuhan,430070.

polyamine oxidases(PAOs) are FAD-dependent enzymes involved in polyamine *** plants,increasing evidence supports that PAOs play an essential role in abiotic and biotc stress *** this study,we investigated 6 putative polyamine oxidases genes from Citrus sinensis genome and compared them with those from *** provide a comprehensive overview of the polyamine oxidases genes family in citrus sinensis(CsPAOs),describing the gene structure,promoter cis-regulatory elements,phylogeny,genome localization,Organ-specific expression and gene expression patterns under stress and hormone ***-regulatory elements prediction showed that 175 putative cis elements more than 6 bp in length were identified in the 1.5 kb of upstream promoter regions,including stress response and hormone response elements. Gene expression patterns analysis found that most of these polyamine oxidase genes were induced by low temperature,salt,drought and ABA *** the CsPAOs were positively induced except for CsPA06 by low *** transcript level of CsPA06 decreased after cold *** respect to NaCl and drought treatment,the expression levels of most of the CsPAOs significantly *** expression pattens under ABA treatment were similar to those under NaCl and drought treatment,and most of the CsPAOs were negatively induced by ABA,suggesting that ABA may be involved in polyamines catabolism.

关键词： Citrus sinensis polyamine oxidase abiotic stress abscisic acid stress response gene expression

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1109-1124页

作者： Yeyao Du Yang Song Ye Zhang Sujie Fan Hanzhu Zhang Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun130118China

polyamines play an important regulatory role during plant growth and development and adversity stress,and polyamine oxidase(PAO)is involved in polyamine *** this study,an up-regulated polyamine oxidase gene GmPAO1 was obtained by transcriptome sequencing analysis and screening at soybean seedling ***,its expression pattern and function were *** identification results of transgenic GmPAO1 soybean positive lines showed that the relative expression level of GmPAO1 in the overexpressed lines was increased under salt *** increasing stress concentration,the seed germination rate ***,the seed germination rate of the overexpressed lines was significantly higher than that of the control lines,and the phenotypic character of the root systems was also better than that of the control *** measurement of superoxide dismutase(SOD)and peroxidase(POD)activities and malondialdehyde and hydrogen peroxide contents revealed that the overexpressed soybean lines significantly increased the SOD and POD activities,significantly reducing the malondialdehyde *** the hydrogen peroxide content in the transformed plants gradually increased,the hydrogen peroxide content in the overexpression lines was still lower than that in the gene editing *** on this,it was preliminarily judged that GmPAO1 can improve soybean salt tolerance.

关键词： Soybean polyamine oxidase salt tolerance overexpressed

Putrescine, spermidine, and spermine play distinct roles in rice salt tolerance

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Journal of Integrative Agriculture 2020年第3期19卷 643-655页

作者： Md Azizul ISlam PANG Jin-huan MENG Fan-wei LI Ya-wen XU Ning YANG Chao LIU Jun State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101P.R.China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049P.R.China Department of Biotechnology and Genetic Engineering Faculty of Applied Science and TechnologyIslamic UniversityKushtia 7003Bangladesh

polyamines(PAs) play diverse roles in plant growth and development,as well as responses to environmental *** this study,the effects of PAs on rice salt tolerance were *** stress resulted in the alteration of endogenous PAs levels in rice roots and leaves,where spermidine(Spd) and spermine(Spm) contents were increased,and putrescine(Put) content was ***-qPCR analysis revealed that PAs biosynthesis-related genes ADC1,ODC,and Arginase were significantly downregulated by salt treatment;however,SAMDC transcription was significantly *** Spm enhanced rice salt tolerance remarkably;however,exogenous Put and Spd undermined rice salt *** rice plants overexpressing SAMDC display a higher ratio of Spm/(Put+Spd) and enhanced salt *** stress also increased polyamine oxidase activities in rice,resulting in elevated reactive oxygen species(ROS) *** findings revealed that accumulation of Put and Spd substantially reduced salt tolerance in rice,likely by facilitating ROS production;whereas,conversion of Put and Spd to Spm contributes to rice salt tolerance.

关键词： rice polyamines NaCl polyamine oxidase guazatine