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Mutations in FgPrp6 suppressive to the Fgprp4 mutant in Fusarium graminearum

Journal of Integrative Agriculture 2022年第5期21卷 1375-1388页

作者： LI Chao-hui FAN Zhi-li HUANG Xin-yi WANG Qin-hu JIANG Cong XU Jin-rong JIN Qiao-jun NWAFU-PU Joint Research Center/State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYang ling 712100P.R.China Institute of plant Protection/Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Department of Botany and plant Pathology Purdue UniversityWest LafayetteIN47907USA

The pre-mRNA processing factor Prp6 is an essential component of the U4/U6.U5 tri-small nuclear ribonucleoprotein(snRNP).In a previous study,mutations were identified in the PRP6 ortholog in four suppressors of Fgprp4 that was deleted of the only kinase FgPrp4 among the spliceosome components in the plant pathogenic fungus Fusarium graminearum.In this study,we identified additional suppressor mutations in FgPrp6 and determined the suppressive effects of selected mutations.In total,12 mutations of FgPRP6 were identified in 20 suppressors of Fgprp4 by sequencing analysis.Whereas three mutation sites are in the linker region of FgPrp6,seven are in the first two HAT repeats.RNA-seq analysis showed that suppressor mutations on different sites caused different splicing efficiency recovery.The suppressive effects of E308K and R230H were verified.Similar to human and fission yeast,the FgPrp6 was phosphorylated by the FgPrp4 kinase.Interestingly,the conserved Prp4-phosphorylation sites T261,T219&T221,and predicted phosphorylation sites T199&T200 on FgPrp6 were dispensable for the function of FgPrp6 in hyphal growth and sexual reproduction but important in plant infection.They are required for the infectious growth of F.graminearum in wheat lemma.RNA-seq analysis of the wheat lemma infected with Fgprp6/FgPRP6^(Δ199-221)-GFP or Fgprp6/FgPRP6^(Δ250-262)-GFP showed that 28 and 35% introns had splicing defects,respectively,which may be responsible for their defects in plant infection.

关键词： RNA splicing FgPrp6 suppressor plant infection phosphorylation

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FgCDC14 regulates cytokinesis,morphogenesis,and pathogenesis in Fusarium graminearum

FgCDC14 regulates cytokinesis,morphogenesis,and pathogenesis...

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中国植物病理学会2015年学术年会

作者： LI Chao-hui ZHANG Shi-jie Michael Melesse HAO Chao-Feng WANG Chen-fang Mark C.Hall XU Jin-Rong State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest Agricultural and Forestry University Dept.of Botany and plant Pathology Purdue University Dept.of Biochemistry Purdue University

Members of Cdc14 phosphatases are common in animals and fungi but absent in plants.Although its orthologs are conserved in plant pathogenic fungi,their functions during infection are not clear.In this study,we showed that CDC14 is important for pathogenesis and morphogenesis in two fungal pathogens.In Fusarium graminearum,FgCDC14 is required for normal cell division and septum formation and FgCdc14 possesses phosphatase activity with specificity for a subset of yeast Cdk-type phosphorylation sites.The Fgcdc14 mutant was reduced in growth,conidiation,and ascospore formation.It was defective in ascosporogenesis and pathogenesis.Septation in Fgcdc14 was reduced and hyphal compartments contained multiple nuclei,indicating defects in the coordination between nuclear division and cytokinesis.Interestingly,foot cells of mutant conidia often differentiated into conidiogenous cells,resulting in the production of inter-connected conidia.In the interphase,FgCdc14-GFP localized to the nucleus and spindle-pole-body.In M.oryzae,deletion of MoCDC14 resulted in similar defects in growth,conidiation,and septation.The Mocdc 14 mutant was defective in appressorium morphogenesis and non-pathogenic.Taken together,our results indicate that Cdcl4 phosphatase functions in cell division and septum formation in F.graminearum and M.oryzae,likely by counteracting Cdk phosphorylation,and is required for plant infection.

关键词： Cell cycle Cytokinesis plant infection Conidiation Sexual reproduction Gibberella zeae

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FgSsn3 kinase,a component of the mediator complex,is important for sexual reproduction and pathogenesis in Fusarium graminearum

FgSsn3 kinase,a component of the mediator complex,is importa...

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中国植物病理学会2015年学术年会

作者： CAO Shu-lin ZHANG Shi-jie LIU Hui-quan HAO Chao-feng JIN Qiao-jun XU Jin-rong State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest Agricultural and Forestry University Dept.of Botany and plant Pathology Purdue University

Fusarium graminearum,an important plant pathogen of wheat and barley,not only causes severe yield losses but also produces harmful mycotoxins contaminating infested cereal.In this study,we characterized the FgSSN3 gene in F.graminearum which is orthologous of SSN3 in Saccharomyces cerevisiae,SRBIO in Saccharomyces pombe and Neurospora crassa.The Fgssn3 deletion mutant is decreased in vegetative growth,abnormal colony appearance and significant reduced in virulence on wheat heads and maize silks.Scanning and transmission electron microscopy（SEM and TEM） results indicate that the mutant Fgssn3 failed to spread from the inoculation site to other parts of wheat heads.Although it is defective in conidium morphology,the Fgssn3 is normal in conidiation.Deletion of FgSSN3 resulted in increased expression levels of HTF1 and PCS1.In addition,the Fgssn3 mutant is defective in sexual reproduction and essential for female fertility but dispensable for male fertility.FgSsn3 physically interacted with FgSsn8 in yeast two-hybrid assays.Results indicate that FgSsn3 as a subunit of mediator complex in F.graminearum regulates growth,development,sexual reproduction and plant infection and contributes to transcriptional repression or activation of diversely regulated genes.

关键词： Mediator complex plant infection Conidiation Sexual reproduction Gibberella zeae

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