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The ceRNA Network Indicates Its Immune Function in peripheral blood leukocytes of Half-Smooth Tongue Sole(Cynoglossus semilaevis)After Stimulation with Chitosan Oligosaccharide

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Journal of Ocean University of China 2024年第6期23卷 1615-1629页

作者： WANG Wenwen WEI Shu CHEN Yadong TAN Suxu SHI Kunpeng ZANG Shaoqing WANG Minmin YANG Teng YANG Yingming CUI Zhongkai SHA Zhenxia Institute of Aquatic Biotechnology College of Life SciencesQingdao UniversityQingdao 266071China State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology CenterQingdao 266237China

As an efficient immunostimulant,chitosan oligosaccharide(COS)can enhance the immunity of teleosts;however,the underlying molecular mechanisms still require *** endogenous RNAs(ceRNAs)are vital regulators in the immune response,but their roles in half-smooth tongue sole(Cynoglossus semilaevis)remain *** this study,for the first time,we studied whole-transcriptome expression profiles and analyzed ceRNA networks in peripheral blood leukocytes of half-smooth tongue sole treated with COS.A total of 19 circRNAs(DE-circRNAs),18 miRNAs(DE-miRNAs)and 50 previously identified lncRNAs(DElncRNAs)were differentially expressed after COS *** DE-lncRNAs and DE-miRNAs targeted numerous immunity-related genes,and were enriched in important pathways,including MAPK and Toll-like receptor signaling pathways,suggesting the immunoregulatory roles of ***,we constructed circRNA-miRNA-mRNA and lncRNA-miRNA-mRNA regulatory networks using DE-circRNAs,DE-miRNAs,DE-lncRNAs,and ***,a ceRNA network with immunity-related DEmRNAs was constructed,showing that 3 DE-circRNAs,12 DE-lncRNAs,and 29 DEGs exhibited crosstalk through 9 ***,a DE-miRNA in the ceRNA network,miR-144-3p,was targeted by DE-lncRNA tnrc6a,and negatively regulated the genes of inhibitor of nuclear factor kappa B kinases(IKKs)(ikbkg,ikbkb,and ikbip)and ***,ikbkb,and c3ar1 were significantly up-regulated in macrophages stimulated by *** could be inferred that ncRNAs participated in the immune and inflammatory response by acting as ceRNAs after COS stimulation in *** findings indicate that COS could enhance the immunity of teleosts by regulating ncRNAs,and lay the foundation for further practical application of COS in aquaculture.

关键词： ceRNA circRNA miRNA half-smooth tongue sole peripheral blood leukocytes chitosan oligosaccharide

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Methylation status of DJ-1 in leukocyte DNA of Parkinson’s disease patients

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Translational Neurodegeneration 2016年第1期5卷 33-39页

作者： Yuyan Tan Li Wu Dunhui Li Xiaoli Liu Jianqing Ding Shengdi Chen Department of Neurology and Institute of NeurologyRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Neurology Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200011China Parkinson’s Disease Center Beijing Institute for Brain DisordersBeijing 100069China

Background:DJ-1 has been thought as a candidate biomarker for Parkinson’s disease(PD).It was found reduced in PD brains,CSF and saliva,although there were conflicting *** DJ-1 expression may be regulated is not ***,blood-based DNA methylation represents a highly promising biomarker for PD by regulating the causative gene ***,in this study,we try to explore whether blood-based DNA methylation of DJ-1 could be used as a biomarker to differentiate PD patients from normal control(NC),and whether DNA methylation could regulate DJ-1 expression in a SH-SY5Y cell ***:Forty PD patients and 40 NC were recruited in this *** was extracted from peripheral blood leukocytes(PBLs).Methylation status of two CpG islands(CpG1 and CpG2)in promoter region of DJ-1 was explored by bisulfite specific PCR-based sequencing *** inhibitor 5-Aza-dC was used to treat SH-SY5Y cell line,DJ-1 level was detected in both mRNA and protein ***:CpG sites in these two CpG islands(CpG1 and CpG2)of DJ-1 were unmethylated in both PD and NC *** SH-SY5Y cell model treated by methylation inhibitor,there was no significant change of DJ-1 expression in either mRNA level or protein ***:Our results indicated that DNA methylation inhibitor didn’t alter DJ-1 gene expression in SH-SY5Y cell model,and DNA methylation of DJ-1 promoter region in PBLs level might not be an efficient biomarker for PD patients.

关键词： Parkinson’s disease DJ-1 DNA methylation peripheral blood leukocytes

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