The ceRNA Network Indicates Its Immune Function in Peripheral Blood Leukocytes of Half-Smooth Tongue Sole(Cynoglossus semilaevis)After Stimulation with Chitosan Oligosaccharide

作     者：WANG Wenwen WEI Shu CHEN Yadong TAN Suxu SHI Kunpeng ZANG Shaoqing WANG Minmin YANG Teng YANG Yingming CUI Zhongkai SHA Zhenxia WANG Wenwen;WEI Shu;CHEN Yadong;TAN Suxu;SHI Kunpeng;ZANG Shaoqing;WANG Minmin;YANG Teng;YANG Yingming;CUI Zhongkai;SHA Zhenxia

作者机构：Institute of Aquatic BiotechnologyCollege of Life SciencesQingdao UniversityQingdao 266071China State Key Laboratory of Mariculture Biobreeding and Sustainable GoodsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao Marine Science and Technology CenterQingdao 266237China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2024年第23卷第6期

页      面：1615-1629页

核心收录：

学科分类：0401[教育学-教育学] 04[教育学] 

基　　金：supported by the National Key Research and Development Project of China(No.2022YFD2400401) the Key Research and Development Project of Shandong(No.2021LZGC028) the Academician Special Program of Shandong Province(No.2023ZLYS02) 

主　　题：ceRNA circRNA miRNA half-smooth tongue sole peripheral blood leukocytes chitosan oligosaccharide 

摘      要：As an efficient immunostimulant,chitosan oligosaccharide(COS)can enhance the immunity of teleosts;however,the underlying molecular mechanisms still require *** endogenous RNAs(ceRNAs)are vital regulators in the immune response,but their roles in half-smooth tongue sole(Cynoglossus semilaevis)remain *** this study,for the first time,we studied whole-transcriptome expression profiles and analyzed ceRNA networks in peripheral blood leukocytes of half-smooth tongue sole treated with COS.A total of 19 circRNAs(DE-circRNAs),18 miRNAs(DE-miRNAs)and 50 previously identified lncRNAs(DElncRNAs)were differentially expressed after COS *** DE-lncRNAs and DE-miRNAs targeted numerous immunity-related genes,and were enriched in important pathways,including MAPK and Toll-like receptor signaling pathways,suggesting the immunoregulatory roles of ***,we constructed circRNA-miRNA-mRNA and lncRNA-miRNA-mRNA regulatory networks using DE-circRNAs,DE-miRNAs,DE-lncRNAs,and ***,a ceRNA network with immunity-related DEmRNAs was constructed,showing that 3 DE-circRNAs,12 DE-lncRNAs,and 29 DEGs exhibited crosstalk through 9 ***,a DE-miRNA in the ceRNA network,miR-144-3p,was targeted by DE-lncRNA tnrc6a,and negatively regulated the genes of inhibitor of nuclear factor kappa B kinases(IKKs)(ikbkg,ikbkb,and ikbip)and ***,ikbkb,and c3ar1 were significantly up-regulated in macrophages stimulated by *** could be inferred that ncRNAs participated in the immune and inflammatory response by acting as ceRNAs after COS stimulation in *** findings indicate that COS could enhance the immunity of teleosts by regulating ncRNAs,and lay the foundation for further practical application of COS in aquaculture.