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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1749-1765页

作者： Yi Xi Li Zhang Yanhong Xu Wei Cheng Chao Chen College of Animal Science Guizhou UniversityGuiyang550025China

In artiﬁcially controlled pot experiments,perennial ryegrass was mixed with other leguminous plants(white clo-ver and alfalfa)and treated with lead,zinc and cadmium(337 mg·kg^(-1),648 mg·kg^(-1),and 9 mg·kg^(-1),respectively)to simulate compound pollution *** results showed that the concentrations of heavy metals,trans-port factors,and bioconcentration factors in mixed planting of ryegrass decreased compared with those in *** of whether heavy metal pollution was introduced,mixed planting increased the aboveground and underground biomasses of *** different mixed planting treatments had no signiﬁcant impact on the chlorophyll concentration of *** mowing time,mixed planting treatment,and heavy metal treatment had impacts on antioxidant and osmotic adjustment substances,and there were some *** mixed planting treatment did not signiﬁcantly affect glutathione concentration,cysteine concentration,or nonprotein *** planting generally increased the nitrogen and phosphorus concentrations of ryegrass while reducing the stoichiometric ratio of carbon,nitrogen,and *** results suggest that the mixed planting of ryegrass with legumes promotes the growth of ryegrass in the presence of high concentrations of heavy metal ***,it does not enhance the ability of ryegrass to remediate heavy metal pollution in the soil.

关键词： perennial ryegrass mixed planting phytoremediation lead zinc and cadmium soil pollution

Removal of nitrogen from wastewater with perennial ryegrass/artificial aquatic mats biofilm combined system

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Journal of Environmental Sciences 2013年第4期25卷 670-676页

作者： Chongjun Chen Rui Zhang Liang Wang Weixiang Wu Yingxu Chen The Academy of Water Science and Environmental Engineering Zhejiang University Institute of Environmental Science and Technology Zhejiang University Zhejiang Provincial Key Laboratory for Water Pollution Control and Environmental Safety

To develop a cost-effective combined phytoremediation and biological process, a combined perennial ryegrass/artificial aquatic mat biofilm reactor was used to treat synthetic wastewater. Influent ammonium loading, reflux ratio, hydraulic retention time （HRT） and temperature all had significant effects on the treatment efficiency. The results indicated that the effluent concentration of ammonium increased with increasing influent ammonium loading. The reactor temperature played an important role in the nitrification process. The ammonium removal efficiency significantly decreased from 80% to 30%-50% when the reactor temperatttre dropped to below 10℃. In addition, the optimal nitrogen removal condition was a reflux ratio of 2. The nitrate and ammonium concentration of the effluent were consistent with the HRT of the combined system. The chemical oxygen demand （COD） removal efficiency was at a high level during the whole experiment, being almost 80% after the start-up, and then mostly above 90%. The direct uptake of N by the perennial ryegrass accounted for 18.17% of the total N removal by the whole system. The perennial ryegrass absorption was a significant contributor to nitrogen removal in the combined system. The result＇illustrated that the combined perennial ryegrass/artificial aquatic mat biofilm reactor demonstrated good performance in ammonium, total N and COD removal.

关键词： nitrogen perennial ryegrass biofilm wastewater treatment

Dissipation of polycyclic aromatic hydrocarbons and microbial activity in a field soil planted with perennial ryegrass

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Frontiers of Environmental Science & Engineering 2012年第3期6卷 330-335页

作者： Dengqiang FU Ying TENG Yuanyuan SHEN Mingming SUN Chen TU Yongming LUO Zhengao LI Peter CHRISTIE Key Laboratory of Soil Environment and Pollution Remediation Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China Agri-Environment Branch Agri-Food and Biosciences InstituteNewforge LaneBelfast BT95PXUK

Dissipation and plant uptake of polycyclic aromatic hydrocarbons （PAHs） in contaminated agricul- tural soil planted with perennial ryegrass were investigated in a field experiment. After two seasons of grass cultivation the mean concentration of 12 PAHs in soil decreased by 23.4% compared with the initial soil. The 3-, 4-, 5-, and 6- ring PAHs were dissipated by 30.9%, 25.5%, 21.2%, and 16.3% from the soil, respectively. ryegrass shoots accumulated about 280 ***1, shoot dry matter biomass reached 2.48 x 104kg-ha1, and plant uptake accounted for about 0.99% of the decrease in PAHs in the soil. Significantly higher soil enzyme activities and microbial community functional diversity were observed in planted soil than that in the unplanted control. The results suggest that planting ryegrass may promote the dissipation of PAHs in long-term contaminated agricultural soil, and plant-promoted microbial degradation may be a main mechanism of phytoremediation.

关键词： perennial ryegrass polycyclic aromatic hydrocarbon bioremediation plant uptake soil microbial activity

Determining the earliest growth stage to detect the presence of endophytes in tall fescue and perennial ryegrass seedlings using molecular markers

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Grassland Research 2023年第2期2卷 106-111页

作者： Kendall Lee Nicholas Hill Chloe Dela Cerna Ali Missaoui Institute of Plant Breeding Genetics and GenomicsUniversity of GeorgiaAthensGeorgiaUSA Department of Crop and Soil Sciences University of GeorgiaAthensGeorgiaUSA

Background:Tall fescue(Festuca arundinacea[Schreb.],Lolium arundinaceum[Schreb.]Darbysh)and perennial ryegrass(Lolium perenne)are important cool-season forage and amenity grasses that have a mutualistic association with an endophytic *** confer insect and drought resistance to plants but can produce mammalian *** endophytes that do not produce mammalian toxins have been introduced to elite cultivars for commercial *** companies need to maintain adequate levels of novel endophytes within the elite forage *** detection is performed using immunochemical and molecular techniques because of their speed and *** detection in seedlings is essential to evaluate the viability of the endophyte within seed ***:This research aimed to identify the earliest growth stage in which immunochemical and molecular methods can detect viable endophyte in seedlings of tall fescue cultivars BarOptima(e34),Texoma MaxQII(584),and Jesup MaxQ(542),as well as the perennial ryegrass cultivar Remington(NEA2).Results:Immunochemical testing detected endophytes in seedlings 14 days after germination(DAG),but the detection rate increased until 42 DAG in some cultivars *** molecular marker Tef1exon detected endophytes at a lower rate than the immunochemical method at 28–42 ***,there was insufficient DNA to detect endophytes in 14 DAG seedlings using ***:We conclude that the most accurate detection of viable endophytes in seedlings was 42 DAG,at which sufficient and consistent endophyte colonization occurred.

关键词： endophyte immunoblot assay molecular markers perennial ryegrass tall fescue

Identification of perennial ryegrass varieties by ultrathin-Iayer isoelectric focusing （UTLIEF） of seed storage protein

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Identification of perennial ryegrass varieties by ultrathin-...

Selection of Rhizosphere Phosphate-solubilizing Bacteria of perennial ryegrass

ⅩⅪ International Grassland Congress and the Ⅷ International Rangeland Congress

作者： LIU Min-Xuan WANG Yun-Wen HAN Jian-Guo~* Institute of Grassland Science China Agricultural University Beijing 100094 China

＜正＞In this work,seed prolamin variation between tested perennial ryegrass varieties was analyzed by ultrathin-layer isoelectric focusing electrophoresis（UTLIEF） for variety *** results showed that UTLIEF is a sui... 详细信息

关键词： perennial ryegrass variety identification UTLIEF

来源： cnki会议评论

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Agricultural Science & Technology 2016年第3期17卷 756-759页

作者：杨明举丁磊磊李世歌莫本田舒健虹张文李娟贵州省水产研究所贵州贵阳550006 贵州省草业研究所贵州贵阳550006

The research isolated phosphorus-soluble bacteria from different parts of ryegrasses and selected 7 bacteria performing better in solbilizing capacity. The test results showed that the capacity of phosphorus-solubilizing tended to be volatile in the range from 135.27 to 187.87 μg/ml and the secreting capacity of IAA was in3.47-24.27 μg/ml. It is believed that Lp59, Lp61, Lp65, Lp69, Lp70 and Lp72 are potential for further development.

关键词： perennial ryegrass Phosphate-solubilizing bacteria Phosphorus-solubilizing ability IAA

Callus Induction and Plant Regeneration from Mature Seeds of perennial ryegrass

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Agricultural Science & Technology 2009年第6期10卷 33-36页

作者：曾升坚卞建春方钲张冬霞邹新海扬州大学兽医学院江苏扬州225009

Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.

关键词： perennial ryegrass Mature seed: Tissue culture Plant regeneration

Effects of daughter tiller removal on shoot and root growth of the parent tiller in Lolium perenne

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Grassland Research 2022年第2期1卷 103-110页

作者： Arif Hasan Khan Robin Cory Matthew The School of Agriculture and Environment College of SciencesMassey UniversityPalmerston NorthNew Zealand Department of Genetics and Plant Breeding Bangladesh Agricultural UniversityMymensinghBangladesh

Background:This study investigated the effects of daughter tiller removal on parent tiller development in Lolium ***:Plants of *** were grown hydroponically to allow separation of roots by phytomer position on the tiller axis and allowed to form two daughter *** Experiment 1,adult daughter tillers were excised and effects on subsequent main tiller growth were observed for 16 days,on *** Experiment 2,the growth of main tillers with or without daughter tillers was compared over 90–100 *** cultivars,‘Alto’bred from New Zealand germplasm and‘Aberdart’bred from United Kingdom germplasm were ***:Excision of adult daughter tillers reduced the dry weight of older roots at the base of parent tiller axes and accelerated new phytomer *** tillers from forming daughter tillers by new tiller excision resulted in increased individual leaf and root dry weight in tillers without daughter ***:The data indicated that daughter tillers contributed substrate for root development of their parent *** presence of daughter tillers reduced the size of their main tiller but greatly enhanced whole plant yield.

关键词： daughter tiller dry weight main tiller perennial ryegrass phytomer

Forage Species Suitability Mapping for China Using Topographic, Climatic and Soils Spatial Data and Quantitative Plant Tolerances

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Agricultural Sciences in China 2005年第9期4卷 660-667页

作者： David B Hannaway Christopher Daly CAO Wei-xing LUO Wei-hong WEI Yu-rong ZHANG Wei-li XU Ai-guo LU Chang-ai SHI Xue-zheng LI Xiang-lin Oregon State University Corvallis Oregon 97331USA Nanjing Agricultural University Nanjing 210095 P.R. China Meteorological Institute of Inner Mongolia Hohhot 010051 P.R.China Soil ＆ Fertilizer Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 P.R.China Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 P.R.China

Selecting plants adapted to the climatic and soil conditions of specific locations is essential for environmental protection and economic sustainability of agricultural and pastoral systems. This is particularly true for countries like China with a diversity of climates and soils and intended uses. Currently, proper species selection is difficult due to the absence of computer-based selection tools. Climate and soil GIS layers, matched with a matrix of plant characteristics through rules describing species tolerances would greatly improve the selection process. Better matching will reduce environmental hazards and economic risks associated with sub-optimal plant selection and performance. GIS-based climate and soil maps have been developed for China. A matrix of quantitative species tolerances has been developed for example forage species and used in combination with an internet map server that allows customized map creation. A web-based decision support system has been developed to provide current information and links to original data sources, supplementary materials, and selection strategies.

关键词： Geospatial Climate Soils Eco-physiology Internet map server (IMS) Dynamic mapping system Decision support system (DSS) perennial ryegrass Lolium perenne L. Orchardgrass Dactylis glomerata L. Tall rescue Lolium arundinaceum (Schreb.) Darbysh

Some Evidence for Host Specificity in Arbuscnlar　Myc-orrhizas

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Pedosphere 1994年第4期4卷 377-381页

作者： J.B. ROGERS P. CHRISTIE and A. S. LAIDLAWDepartment of Applied Plant Science, The Queen’s University of Belfast, Newforge Lane, Belfast BT95PX(U. K.)Department of Agricnltural Chemistry, The Queen’s University of Belfast, Newforge Lane, Belfnst BT

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关键词： arbuscular mycorrhizas host specificity perennial ryegrass white clover