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Chromatographic and mass spectroscopic guided discovery of Trichoderma peptaibiotics and their bioactivity

Engineering Microbiology 2024年第2期4卷 62-76页

作者： Adigo Setargie Chen Wang Liwen Zhang Yuquan Xu Biotechnology Research Institute The Chinese Academy of Agricultural Sciences12 Zhongguancun South StreetBeijing 100081China Institute of Biotechnology Bahir Dar UniversityP.O.Box.79Bahir DarEthiopia

peptaibiotics are linear or cyclic peptide antibiotics characterized by the non-proteinogenic amino acid,alpha-aminoisobutyric *** exhibit a wide range of bioactivity against various *** report presents a comprehensive review of analytical methods for Trichoderma cultivation,production,isolation,screening,pu-rification,and characterization of peptaibiotics,along with their *** techniques are currently available for each step,and we focus on describing the most commonly used and recently developed chromato-graphic and spectroscopic *** peptaibiotics requires efficient culture media,growth con-ditions,and isolation and purification *** combination of chromatographic and spectroscopic tools offers a better opportunity for characterizing and identifying *** evaluation of the chemical and biological properties of this compound has also been explored concerning its potential application in pharma-ceutical and other *** review aims to summarize available data on the techniques and tools used to screen and purify peptaibiotics from Trichoderma fungi and bioactivity against various pathogens.

关键词： Chromatography peptaibiotics spectroscopy Trichoderma

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Front line defenders of the ecological niche!Screening the structural diversity of peptaibiotics from saprotrophic and fungicolous Trichoderma/Hypocrea species

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Fungal Diversity 2014年第6期69卷 117-146页

作者： Christian R.Rohrich Walter M.Jaklitsch Hermann Voglmayr Anita Iversen Andreas Vilcinskas Kristian Fog Nielsen Ulf Thrane Hans von Dohren Hans Bruckner Thomas Degenkolb Bioresources Project Group Fraunhofer Institute for Molecular Biology and Applied Ecology(IME)Winchesterstrasse 235394 GiessenGermany Department of Systematic and Evolutionary Botany Faculty Centre of BiodiversityUniversity of ViennaRennweg 141030 ViennaAustria Department of Systems Biology Technical University of DenmarkSoltofts PladsBuilding 2212800 Kgs.LyngbyDenmark Interdisciplinary Research Centre for BioSystems Land Use and Nutrition(IFZ)Department of Applied EntomologyInstitute of Phytopathology and Applied Zoology(IPAZ)University of GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany Biochemistry and Molecular Biology OE 2 Institute of ChemistryTechnical University of BerlinFranklinstrasse 2910587 BerlinGermany Interdisciplinary Research Centre for BioSystems Land Use and Nutrition(IFZ)Department of Food SciencesInstitute ofNutritional ScienceUniversity of GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany AB SCIEXGermany GmbH Landwehrstrasse 5464293 DarmstadtGermany Danish Emergency Management Agency Universitetsparken 22100 CopenhagenDenmark

Approximately 950 individual sequences of nonribosomally biosynthesised peptides are produced by the genus Trichoderma/Hypocreathat belong to a perpetually growing class of mostly linear antibiotic oligopeptides,which are rich in the non-proteinogenic α-aminoisobutyric acid(Aib).Thus,they are comprehensively named ***,peptaibiotics represent ca.80% of the total inventory of secondarymetabolites currently known from Trichoderma/*** unique membrane-modifying bioactivity results from amphipathicity and helicity,thus making them ideal candidates in assisting both colonisation and defence of the natural habitats by their fungal *** this,reports on the in vivo-detection of peptaibiotics have scarcely been published in the *** order to evaluate the significance of peptaibiotic production for a broader range of potential producers,we screened nine specimens belonging to seven hitherto uninvestigated fungicolous or saprotrophic Trichoderma/Hypocrea species by liquid chromatography coupled to electrospray high resolution mass *** of peptaibiotics found were independently confirmed by analysing the peptaibiome of pure agar cultures obtained by single-ascospore isolation from the *** the nine species examined,five were screened positive for peptaibiotics.A total of 78 peptaibiotics were sequenced,56(=72%)of which are ***,dihydroxyphenylalaninol and O-prenylated tyrosinol,two C-terminal residues,which have not been reported for peptaibiotics before,were found as well as new and recurrent sequences carrying the recently described tyrosinol residue at their *** majority of peptaibiotics sequenced are 18-or 19-residue *** homologies with‘classical representatives’of subfamily 1(SF1)-peptaibiotics argue for the formation of transmembrane ion channels,which are prone to facilitate the producer capture and defence of its substratum.

关键词： HPLC/QTOF-ESI-HRMS Metabolite profiling peptaibiotics Peptaibols Aib peptides Trichoderma Hypocrea

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