Front line defenders of the ecological niche!Screening the structural diversity of peptaibiotics from saprotrophic and fungicolous Trichoderma/Hypocrea species

作     者：Christian R.Rohrich Walter M.Jaklitsch Hermann Voglmayr Anita Iversen Andreas Vilcinskas Kristian Fog Nielsen Ulf Thrane Hans von Dohren Hans Bruckner Thomas Degenkolb 

作者机构：Bioresources Project GroupFraunhofer Institute for Molecular Biology and Applied Ecology(IME)Winchesterstrasse 235394 GiessenGermany Department of Systematic and Evolutionary BotanyFaculty Centre of BiodiversityUniversity of ViennaRennweg 141030 ViennaAustria Department of Systems BiologyTechnical University of DenmarkSoltofts PladsBuilding 2212800 Kgs.LyngbyDenmark Interdisciplinary Research Centre for BioSystemsLand Use and Nutrition(IFZ)Department of Applied EntomologyInstitute of Phytopathology and Applied Zoology(IPAZ)University of GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany Biochemistry and Molecular Biology OE 2Institute of ChemistryTechnical University of BerlinFranklinstrasse 2910587 BerlinGermany Interdisciplinary Research Centre for BioSystemsLand Use and Nutrition(IFZ)Department of Food SciencesInstitute ofNutritional ScienceUniversity of GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany AB SCIEXGermany GmbHLandwehrstrasse 5464293 DarmstadtGermany Danish Emergency Management AgencyUniversitetsparken 22100 CopenhagenDenmark 

出 版 物：《Fungal Diversity》 (真菌多样性（英文）)

年 卷 期：2014年第69卷第6期

页      面：117-146页

核心收录：

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

基　　金：supported by the Hessian Ministry for Science and Art by a grant from the LOEWE-Schwerpunkt‘Insect Biotechnology’to Andreas Vilcinskas the grant from the Danish Research Council(FI 2136-08-0023)for the maXis QTOF system MYCORED(EC KBBE-2007-222690-2)for supporting Anita Iversen the support by the Austrian Science Fund(project P22081-B17) 

主　　题：HPLC/QTOF-ESI-HRMS Metabolite profiling Peptaibiotics Peptaibols Aib peptides Trichoderma Hypocrea 

摘      要：Approximately 950 individual sequences of nonribosomally biosynthesised peptides are produced by the genus Trichoderma/Hypocreathat belong to a perpetually growing class of mostly linear antibiotic oligopeptides,which are rich in the non-proteinogenic α-aminoisobutyric acid(Aib).Thus,they are comprehensively named ***,peptaibiotics represent ca.80% of the total inventory of secondarymetabolites currently known from Trichoderma/*** unique membrane-modifying bioactivity results from amphipathicity and helicity,thus making them ideal candidates in assisting both colonisation and defence of the natural habitats by their fungal *** this,reports on the in vivo-detection of peptaibiotics have scarcely been published in the *** order to evaluate the significance of peptaibiotic production for a broader range of potential producers,we screened nine specimens belonging to seven hitherto uninvestigated fungicolous or saprotrophic Trichoderma/Hypocrea species by liquid chromatography coupled to electrospray high resolution mass *** of peptaibiotics found were independently confirmed by analysing the peptaibiome of pure agar cultures obtained by single-ascospore isolation from the *** the nine species examined,five were screened positive for peptaibiotics.A total of 78 peptaibiotics were sequenced,56(=72%)of which are ***,dihydroxyphenylalaninol and O-prenylated tyrosinol,two C-terminal residues,which have not been reported for peptaibiotics before,were found as well as new and recurrent sequences carrying the recently described tyrosinol residue at their *** majority of peptaibiotics sequenced are 18-or 19-residue *** homologies with‘classical representatives’of subfamily 1(SF1)-peptaibiotics argue for the formation of transmembrane ion channels,which are prone to facilitate the producer capture and defence of its substratum.