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Creation of High-Yield Polyhydroxyalkanoates Engineered Strains by Low Energy Ion Implantation

Plasma Science and Technology 2008年第6期10卷 769-774页

作者：钱时权程郢朱苏文程备久 Anhui Key Laboratory of Biomaterials and Biomass Energy Anhui Agricultural UniversityHefei 230036China Department of Food and BioengineeringBengbu CollegeBengbu 233030China Anhui Key Laboratory of Biomaterials and Biomass Energy Anhui Agricultural UniversityHefei 230036China

Polyhydroxyalkanoates （PHAs）, as a candidate for biodegradable plastic materials, can be synthesized by numerous microorganisms. However, as its production cost is high in comparison with those of chemically synthesized plastics, a lot of research has been focused on the efficient production of PHAs using different methods. In the present study, the mutation effects of PHAs production in strain pCB4 were investigated with implantation of low energy ions. It was found that under the implantation conditions of 7.8×10^14 N^＋/cm^2 at 10 keV, a high-yield PHAs strain with high genetic stability was generated from many mutants. After optimizing its fermentation conditions, the biomass, PHAs concentration and PHAs content of pcbh4 reached 2.26 g/L, 1.81 g/L, and 80.08% respectively, whereas its wild type controls were about 1.24 g/L, 0.61 g/L, and 49.20%. Moreover, the main constituent of PHAs was identified as poly-3-hydroxybutyrates （PHB） in the mutant stain and the yield of this compound was increased up to 41.33% in contrast to that of 27.78% in the wild type strain.

关键词： polyhydroxyalkanoates(PHAs) pcbh4 low energy ion implantation mutation

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