Creation of High-Yield Polyhydroxyalkanoates Engineered Strains by Low Energy Ion Implantation

作     者：钱时权 程郢 朱苏文 程备久 

作者机构：Anhui Key Laboratory of Biomaterials and Biomass EnergyAnhui Agricultural UniversityHefei 230036China Department of Food and BioengineeringBengbu CollegeBengbu 233030China Anhui Key Laboratory of Biomaterials and Biomass EnergyAnhui Agricultural UniversityHefei 230036China 

出 版 物：《Plasma Science and Technology》 (等离子体科学和技术（英文版）)

年 卷 期：2008年第10卷第6期

页      面：769-774页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：Anhui Key Laboratory Foundation of China(No.04swz002) 

主　　题：polyhydroxyalkanoates(PHAs) pCBH4 low energy ion implantation mutation 

摘      要：Polyhydroxyalkanoates （PHAs）, as a candidate for biodegradable plastic materials, can be synthesized by numerous microorganisms. However, as its production cost is high in comparison with those of chemically synthesized plastics, a lot of research has been focused on the efficient production of PHAs using different methods. In the present study, the mutation effects of PHAs production in strain pCB4 were investigated with implantation of low energy ions. It was found that under the implantation conditions of 7.8×10^14 N^＋/cm^2 at 10 keV, a high-yield PHAs strain with high genetic stability was generated from many mutants. After optimizing its fermentation conditions, the biomass, PHAs concentration and PHAs content of pCBH4 reached 2.26 g/L, 1.81 g/L, and 80.08% respectively, whereas its wild type controls were about 1.24 g/L, 0.61 g/L, and 49.20%. Moreover, the main constituent of PHAs was identified as poly-3-hydroxybutyrates （PHB） in the mutant stain and the yield of this compound was increased up to 41.33% in contrast to that of 27.78% in the wild type strain.