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Impact of oxygen supply on production of terpenoids by microorganisms: State of the art

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Chinese Journal of Chemical Engineering 2021年第2期34卷 46-53页

作者： Ting-Ting Liu Han Xiao Jian-Hui Xiao Jian-Jiang Zhong State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesLaboratory of Molecular Biochemical Engineering and Advanced Fermentation TechnologyDepartment of BioengineeringSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Zunyi Municipal Key Laboratory of Medicinal Biotechnology Center for Translational MedicineAffiliated Hospital of Zunyi Medical UniversityZunyi 563003China

Terpenoids are a class of high value-added natural products with a variety of biological *** engineered microorganisms,such as those of Escherichia coli and Saccharomyces cerevisiae,have merits in producing plant or fungus-derived terpenoids,due to their mature genetic manipulation,simple nutrient demand and fast ***,as a key environmental factor,is particularly important to microbial metabolism and growth,and suitable oxygen supply is viewed as a prerequisite for realizing highly efficient production of terpenoids by engineered *** this article,the role of oxygen in regulating terpenoid bioproduction is overviewed from the viewpoints of cellular carbon metabolism,energy metabolism and terpenoid *** on adjusting oxygen availability to microorganisms,including genetic modification of cellular metabolism related with oxygen utilization,are summarized and discussed,to provide helpful information for further improvement of terpenoid biosynthesis by microbes.

关键词： Terpenoid Microorganisms oxygen supply Chassis modification

Effects of H_2O_2 on oxygen supply in the Process of DCA Fermentation

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Tsinghua Science and Technology 2002年第5期7卷 442-447页

作者：黄英明焦鹏李书良华玉涛曹竹安 Department of Chemical Engineering Tsinghua University Beijing 100084 China

Long chain dicarboxylic acid (DCA) produced by Candida tropicalis is produced in an aerobic viscous fermentation system. The gas-liquid transport resistance can be overcome and the oxygen supply can be increased by adding hydrogen peroxide (H_2O_2) to the fermentation system. This paper shows that H_2O_2 can not only enhance the oxygen supply but also change the metabolism by inducing cytochrome P450, the key enzyme of α, ω-oxidation. When C. tropicalis was cultivated in a 3-liter bioreactor using the combination of aeration and hydrogen peroxide feeding, the DCA yield increased about 10% except at the beginning of H_2O_2 feeding. The experiments showed that the maximum activities of P450 could be induced at 2 mmol·L -1 H_2O_2. By adding H_2O_2, the DCA yield in a 22-liter bioreactor was increased 25.3% to 153.9 g/L.

关键词： dicarboxylic acid C. tropicalis oxygen supply hydrogen peroxide cytochrome P450

Causes and detection of coalfield fires, control techniques, and heat energy recovery: A review

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International Journal of Minerals,Metallurgy and Materials 2020年第3期27卷 275-291页

作者： He-tao Su Fu-bao Zhou Bo-bo Shi Hai-ning Qi Jin-chang Deng School of Engineering and Technology China University of Geosciences(Beijing)Beijing 100083China Key Laboratory of Gas and Fire Control for Coal Mines(China University of Mining and Technology) Ministry of EducationXuzhou 221116China Key Laboratory of Deep GeoDrilling Technology Ministry of Natural ResourcesChina University of Geosciences(Beijing)Beijing 100083China

Coalfield fires are considered a global crisis that contributes significantly to environmental destruction and loss of coal resources and poses a serious threat to human safety and health. In this paper, research related to the initiation, development, and evolution of coalfield fires is reviewed. The existing detection and control techniques of coalfield fires are also reviewed. Traditional firefighting is associated with waste of resources, potential risks of recrudescence, potential safety hazards, extensive and expensive engineering works, and power shortages. Recently,coalfield fires have been recognized as having significant potential for energy conservation and heat energy recovery. Thermoelectric power generation is regarded as a suitable technology for the utilization of heat from coalfield fires. The extraction of heat from coalfield fires can also control coalfield fires and prevent reignition leading to combustion. Technologies for absorbing heat from burning coal and overlying rocks are also analyzed. In addition, the control mode of "three-region linkage" is proposed to improve firefighting efficiency. Integrating heat energy recovery with firefighting is an innovative method to control coalfield fires.

关键词： spontaneous coal combustion oxygen supply firefighting thermoelectric thermosyphon three-region linkage

Effects of hypoxic preconditioning on the changes of expression of neuroglobin mRNA and labeled positive cells following cerebral ischemia in gerbils

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Neural Regeneration Research 2006年第5期1卷 397-400页

作者： Yong Zhang Yanqun Chang Zhenfang Liu Qingxi Fu Xiao Zhang Department of Neurology Shandong Provincial HospitalShandong University Jinan250021 Shandong ProvinceChina Depayment of Anesthesiology Shandong Provincial HospitalShandong University Jinan250021 Shandong ProvinceChina

BACKGROUND： Neuroglobin （NGB）, as newly discovered the third member of the globin family binding oxygen, mainly exists in brain of human beings and vertebrates, and it is closely correlated with the oxygen supply in brain. OBJECTIVE： To observe the changes of the expression of neuroglobin and number of positive cells labeled immunohistochemically following cerebral ischemia in gerbils after hypoxic preconditioning. DESIGN ： A complete randomized grouping design and controlled experiment.SETTING ： Department of Neurology, Department of Anesthesia, Shandong Provincial Hospital, Shandong University. MATERIALS ： Sixty-six adult male Mongolian gerbils of clean degree, about 50-65 g, at an average of 57.5 g were provided by the Experimental Animal Center of Capital Medical University [certificate number of animal quality：SCXK（Beijing）2000-0012]. TRNzil （Tianwei Shidai Company, Beijing）, polymerase chain reaction （PCR） primers （synthetized by Invitrogen Company, Shanghai）; reverse transcription-PCR （RT-PCR） one-step kit （Toyobo Company）; PCR instrument （GeneAmp PCR System 240）; mice brain NGB monoclonal antibody （Academy of Military Medical Sciences）; DAB （Zhongshan Company, Beijing）. METHEDS ： The study was completed from December 2004 to June 2005 in Shandong Provincial Hospital. ① The 66 gerbils were randomly divided into sham-operated group （n =6）, cerebral ischemia group （n =30） and hypoxic preconditioning group （n =30）. The gerbils in the hypoxic preconditioning group were put in the environment which contained 02 （0.08 in volume fraction） and N2 （0.92 in volume fraction） at temperature of 25 ℃ for 2 hours. After 5 hours, the gerbils in the hypoxic preconditioning group and cerebral ischemia group were anesthetized, then bilateral common carotid arteries were ligated. In the sham-operated group, bilateral common carotid arteries were only isolated without ligation and hypoxic preconditioning. ② At 1, 5, 10, 30 and 60 minutes after cerebral ischemia, the exp

关键词： cerebral ischemia hypoxic preconditioning mRNA expression Mongolian gerbils carotid arteries volume fraction immunohistochemical staining monoclonal antibody Experimental Animal Medical Sciences oxygen supply human beings