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Effect of oviductus ranae and oviductus ranae Eggs on Bone Metabolism and Osteoporosis

Chinese Journal of Integrative Medicine 2013年第7期19卷 532-538页

作者：王丹辉吴巍田建明王照辉王丹彤项颗朱国英韩铁 Traditional Chinese Medicine Academy of Science of Jilin Province Beijing Tongrentang Biological Product Development Co. Ltd. Institute of Radiation Medicine Fudan University Institute of Military Veterinary Academy of Military Medical Sciences

To evaluate the roles or effects of oviductus ranae （OR） or oviductus ranae eggs （ORE） in preventing and treating postmenopausal osteoporosis. Methods： In vivo experiment： Sixty female adult Wistar rats were randomly divided into 5 groups of 12. To provide an osteoporosis model 4 groups of rats were ovariectomized （OVX）, with the 5th being sham operated. Medication commenced 7 days after the operation and lasted continuously for 12 weeks. Sham operated and OVX groups were given equivalent volumes of 5% Tween-80. The other three groups intragastrically received conjugated estrogens （CE）, OR or ORE of the corresponding doses. At the 12th week, serum estrogen, bone gla protein （BGP）, serum calcium, phosphorus, and alkaline phosphatase （ALP） were assayed; bone mineral densities （BMD） were measured and bone scanning was conducted; uteri were weighed, and weight, volume and length of the femoral bones were determined; and cortical thickness of femoral heads and area of bone trabecula were measured by image analyzer. In vitro experiment： Eighty 10-month old SD rats, with equal numbers of mates and females, were randomly divided into 8 groups. Osteoblasts were isolated from neonatal rat calvariae, and the cells were exposed to various concentrations of serum from OR and ORE groups to study the impact of these sera on osteoblastic proliferation, ALP activity and mineralization. Osteoclastic numbers were determined using tartrate resistant acid phosphatase （TRAP）. Results： In vivo experiment： The body weight of the four OVX groups increased significantly （P〈0.01）. Uterine weight of the CE group was the highest （P〈0.01）; Compared with the model group, estrogen level, BMD, bone scanning/bone imaging index weight of the femoral bones, cortical thickness of femoral heads in the OR and ORE groups increased significantly （P〈0.05, P〈0.01）; femoral volume in the ORE group increased significantly （P〈0.05）; and the content of osteocalcin, phosphorus, and ALP in serum decreased signific

关键词： oviductus ranae oviductus ranae eggs ovariectomized rats osteoblasts osteoclasts,osteoporosis

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林蛙油粗多糖提取条件优化及其药理活性研究

林蛙油粗多糖提取条件优化及其药理活性研究

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作者：杨靖吉林农业大学

学位级别：硕士

In this paper, oviductus ranae from the Changbai Mountains was used to study extracting technology, antineoplastic activity and fatigue resistence under laboratory conditions.1. Comparative test of enzymes:to hydrolyze the dry oviductus ranae, three proteases including pepsin, neutral protease and trypsinase were used to compare the extracting output of crude polysaccharides. Water fraction was set as 1:100, corresponding zymohydrolysis pH was adjusted, trypsinase concentration was 5% and zymohydrolysis time was 2h. After alcohol deposition, extracting output of crude polysaccharides was:0.19%,2.16% and 2.3%. The result showed that trypsinase was a ideal enzyme for crude polysaccharides extrcting from oviductus ranae.2. Optimizing test of extracting technology:first, water fraction, trypsinase concentration, zymohydrolysis time and zymohydrolysis pH were taken as factors to evaluate the best intervals by single-factor examination. Then, further L9(34) orthogonal design trials were carried out. The extracting output and purity of crude polysaccharides were examined. Results showed that extracting condition had significant (PB>D>C. Optimum conditions for the highest output, which was 5.36% and purity was17.40%, was A2B2C3D2, which reffered that the ratio of water to sample was 1:150, trypsinase concentration was 7%;zymohydrolysis time was 8h, and zymohydrolysis pH was 8. Each condition of purity was insignificant (P>0.05), The most important factor was water fraction, zymohydrolysis time came the second, the third was zymohydrolysis pH, and the last was trypsinase concentration, or A>C>D>B. Optimum conditions for the highest purity, which was 24.19% and output was 4.17%, was A3,B3,C3,D3, which reffered that the ratio of water to sample was 1:20

关键词： oviductus ranae crude polysaccharides optimized extracting technology antitumor activity fatigue resistence

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