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Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea

International Journal of Ophthalmology(English edition) 2016年第3期9卷 325-331页

作者： Xian-Ning Liu Xiu-Ping Zhu Jie Wu Zheng-Jie Wu Yong Yin Xiang-HuaXiao Xin Su Bin Kong Shi-Yin Pan Hua Yang Yan Cheng Na An Sheng-Li Mi Shaanxi Institute of Ophthalmology Xi'an 710002 Shaanxi Province China Shaanxi Key Laboratory of Eye Xi'an 710002 Shaanxi Province China Xi'an First Hospital Xi'an 710002 Shaanxi Province China Biomanufacturing Engineering Laboratory Graduate School at Shenzhen Tsinghua University Shenzhen 518055Guangdong Province China

AIM： To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea. METHODS： A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission electron microscopy （TEM） and hematoxylin and eosin （H＆E） staining. The mechanical properties were detected by a rheometer and a tension machine. The acellular corneal matrix was also transplanted into a rabbit cornea and cytokeratin 3 was used to check the immune phenotype, RESULTS： The microstructure and mechanical properties of the ostrich cornea were well preserved after the decellularization process, in vitro, the methyl thiazolyl tetrazoUum results revealed that extracts of the acellular ostrich corneas （AOCs） had no inhibitory effects on the proliferation of the corneal epithelial or endothelial cells or on the keratocytes, The rabbit lamellar keratoplasty showed that the transplanted AOCs were transparent and completely incorporated into the host cornea while corneal turbidity and graft dissolution occurred in the acellular porcine cornea （APC） transplantation, The phenotype of the reconstructed cornea was similar to a normal rabbit cornea with a high expression of cytokeratin 3 in the superficial epithelial cell layer, CONCLUSION： We first used AOCs as scaffolds to reconstruct damaged corneas. Compared with porcine corneas, the anatomical structures of ostrich corneas are closer to those of human corneas. In accordance with the principle that structure determines function, a xenograft lamellar keratoplasty also confirmed that the AOC transplantation generated a superior outcome compared to that of the APC graft.

关键词： ostrich acellular corneal stroma tissue engineering cornea

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ostrich Antibody and Its Application to Skin Diseases, a Review and Case Report

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Health 2018年第10期10卷 1357-1370页

作者： Yasuhiro Tsukamoto Osamu Maeda Genshi Shigekawa Stuart Greenberg Barry Hendler Department of Animal Sciences Graduate School of Environmental & Biological Sciences Kyoto Prefecture University Kyoto Japan Clinical Studies Zeal Cosmetics Osaka Japan Bioresearch Section Maz World Hong Kong Science Park Hong Kong S.A.R. China Clinical Studies Ostri Gen Holdings West Newton MA USA Microfollicular Hair Transplantation Ambler PA USA

Antibodies are immunoglobulins produced by B cells when antigens such as allergens or pathogens invade an animal’s body. The antibodies remove and inactivate antigens. Antibodies are distributed in internal body and mucosal membrane to protect living animals, but they are excellent proteins that can exert their functions, “antigen-antibody reactions,” even when removed from the body. For that reason, antibodies are being put to practical use in diagnostic kits for conditions such as pregnancy and influenza infection, and as anticancer drugs targeting specific tumor markers. The result has been an increasing use of antibodies for research, diagnosis, and therapeutic purposes. Unfortunately, antibodies from experimental mammals such as mice, rats, and rabbits, are not suited to industrial use because of their high production cost. Moreover, handling of these antibodies is difficult due to their vulnerability to heat, acids and alkalis. Accordingly, there is no adaptability to mass production. Recently, we developed a convenient method for the low-cost, mass-production of antibodies using egg-laying hen ostriches. The ostrich egg is an excellent source of antibodies for industrial purposes. The present report shows that the ostrich antibodies have therapeutic effects in ailments such as atopic dermatitis, acne, pyoderma, and pollen allergies. We have successfully produced and purified ostrich immunoglobulin yolk (IgY) against pollen allergens (Cryj1, Cryj2, Chao1, Chao2) and found that allergic reactions were alleviated in skin patch tests of allergic patients by using the ostrich IgY. In addition, we produced ostrich IgY against the homogenates of Staphylococcus aureus and Propionibacterium acnes, and applied to dermal lesions of atopic dermatitis and acne patients, and then observed the therapeutic effects on the dermatitis of volunteer subjects. Antibody against S. aureus also had the therapeutic effect on canine pyoderma caused by MRSA. A particular advantage in us

关键词： ostrich Antibody Cosmetics Atopic Dermatitis Acne Pollen Allergy Pyoderma

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ostrich ocular optics and the design of eyes in terrestrial birds

Ostrich ocular optics and the design of eyes in terrestrial ...

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23rd International Ornithological Congress

作者： Graham R.Martin Gadi Katzir Udi Ashash School of Biosciences The University of Birmingham Birmingham B15 2TT UK Dept. of Biology University of Haifa Oranim 36910 Israel Zemach ostrich Syndicate Jordan Valley Israel

＜正＞ The optical structure of the eye of ostriches （Struthio camelus） was determined by constructing a schematic eye model for paraxial optics. The eye is large （axial length = 38mm） and of globose shape, with an anterior focal length （posterior nodal distance） of 21.8mm. The optical design of the eye is such that the lens and cornea contribute equally to its total optical power. Interspecific comparison shows that, optically, the ostrich eye is a

关键词： vision eye optical structure ostrich

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Protection against Infectious Bronchitis Virus, a Corona Virus Infection, Using ostrich Antibodies

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Health 2018年第10期10卷 1294-1308页

作者： Yasuhiro Tsukamoto Yuna Nakano Kazuhide Adachi Department of Animal Hygiene Graduate School of Environmental & Biological Sciences Kyoto Prefecture University Kyoto Japan

In chickens, infectious bronchitis (IB) is a major respiratory disease. The respiratory system is the primary multiplication site of IB virus (IBV), a coronavirus, after which the virus is distributed to other organs. Poultry farms sustain considerable economic damage due to IB outbreaks in flocks, since IB causes a severe reduction in weight gain in chicks. In the present study, we produced the ostrich IgY against IBV by immunizing female ostriches with the IB viral antigens. The resultant purified IgY showed a strong neutralizing activity against IBV infection of cultured primary chick kidney cells. The infectivity of IBV was markedly inhibited in the trachea of chicks when ostrich IgY was injected intra-muscularly into newly hatched chicks prior to viral inhalation challenge at two weeks of age. Furthermore, the infection was strongly blocked in the tracheae when IgY was injected into chicks at one day and one week of age, with viral inhalation performed at three weeks of age. These findings suggest that the injection of ostrich IgY can help protect young chicks from IBV infections. In south Asian and African countries, broiler chicks are sent to poultry market around 30 days of age, so it is important to prevent IB outbreaks in very young flocks. We strongly believe that ostrich IgY will be a powerful weapon against IB infection in poultry farms on a wide scale and also hope that these findings will aid in the development of antibody vaccines for new type corona viruses, SARS-CoV and MERS-CoV.

关键词： ostrich Antibody Infectious Bronchitis Corona Virus

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Characterization of the Gastric Intraluminal pH and Development of the Proventricular Deep Glands during Perinatal Period in the ostrich

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Journal of Life Sciences 2011年第10期5卷 806-813页

作者： Ilmars Duritis Arnis Mugurevics Preclinical Institute Faculty of Veterinary Medicine Latvia University of Agriculture Jelgava Latvia

In this research, the state of the gastric secretion in 38 days old ostrich embryos and chicks of different age was investigated by determining the pH level in the stomach as well as the histological structure of the deep proventricular glands （gll. proventriculares profundi）. In both parts of the stomach, on the 38th day of embryonic development pH parameters were higher than in chicks after hatching. The proventricular deep gland lobules and secretory epithelium on the 38th day of embryonic development and on the day of hatching were comparatively poorly developed. The most rapid increase of the proportion of the secretory epithelium of the proventricular deep glands was observed just before hatching and during the first week after hatching, but having reached two weeks of age it did not increase any more. The obtained results allow making a conclusion that already on the day of hatching an intensive gastric juice secretion occurs in the proventricular deep glands regardless of the stage of their histological development. The gastric intraluminal pH parameters indicate that the gastric secretion increases with chicks advancing in age, however on the day of hatching this important gastric function is biologically rather complete.

关键词： ostrich gastric intraluminal pH deep proventricular glands.

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Morphological Analysis of the African ostrich Tibia

Morphological Analysis of the African Ostrich Tibia

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中国畜牧兽医学会动物解剖学及组织胚胎学分会第五次会员代表大会暨第十六次学术研讨会

作者： CHENG Jia-yue~(1,2),PENG Ke-mei~(1*),WANG Yun~1,LIU~1,Hua-zhenSONG Hui~1, TANG Zhong-lin~2,YANG Shu-lin~2 1 College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070 2 Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193

To reveal their morphological characteristics,the tibias of four ostriches(one-year-old) were examined in three aspects:bone mineral density(BMD),ultrastructure and *** results indicate that the ostrich tibia has some dramatic *** ostrich fibula shows degeneration and gradual concrescence with the *** addition,the ostrich ankle,the distal end of tibia,cannot *** BMD of the ostrich tibia was also found to be very high,about 4-8-fold greater than human and scanning electron microscopy indicated a firm,strong and non-honeycomb-shaped *** further observed three characteristics of the microstructure:(1) the layers of cortical bone are very clear;(2) the layers of the outer circumferential lamella and inner circumferential lamella are much thicker than those in other animals;and(3) there is a special"transition zone"between the outer circumferential lamella and osteon *** analysis has also revealed that the decalcification of ostrich bone requires more time than other species reported to date.

关键词： ostrich tibia osteon decalcification transition zone

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The Efficacy of ostrich Antibodies to Dihydrotestosterone and 5<i>α</i>-Reductase in the Restoration of Hair Growth

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Health 2019年第10期11卷 1320-1330页

作者： Yasuhiro Tsukamoto Barry Hendler Stuart Greenberg Carol Epstein Department of Animal Sciences Graduate School of Environmental & Biological Sciences Kyoto Prefecture University Kyoto Japan Clinical Studies Ostrich Pharma USA West Newton Massachusetts USA Microfollicular Hair Transplantation Ambler Pennsylvania USA

The hormone dihydro testosterone (DHT) and the catalyst 5α-reductase are key contributors to hair loss due to androgenetic alopecia (AGA). Also, dermal bacteria and their toxins have been implicated in hair loss. A preliminary study by the authors with six male volunteer subjects showed that ostrich antibodies against above causative substances were quite effective in promoting hair regrowth. The present study included 13 men and 4 women, with ages ranging from 39 to 78. Pre- and post-treatment hair counts were also incorporated into the study procedure. In all instances but one, the subjects had a hair-growth effect about 3 months after the antibody was applied to the scalp, and the study participants with hair growth were pleased with the results and with the ease of use of the antibody solution.

关键词： Alopecia AGA ostrich Antibody

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Application of ostrich Antibodies to the Restoration of Hair Growth, a Preliminary and Case Report

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Journal of Cosmetics, Dermatological Sciences and Applications 2018年第4期8卷 179-184页

作者： Yasuhiro Tsukamoto Barry Hendler Stuart Greenberg Department of Animal Sciences Graduate School of Environmental & Biological Sciences Kyoto Prefecture University Kyoto Japan Microfollicular Hair Transplantation Ambler PA USA Clinical Studies OstriGen Holdings West Newton MA USA

The hormone dihydrotestosterone (DHT) and the catalyst 5α-reductase play very important roles in the hair loss due to androgenetic alopecia (AGA). Recently, some chemicals against these hormones have been commercialized as therapeutics for AGA. In the present study, we have attempted to find out the potential effects of antibodies on AGA therapeutics as an inhibitor of androgenic hormones. The immune system of the ostrich is believed to be the most versatile and powerful of any animal on the planet, and antibodies derived from the ostrich egg yolk are now being developed for a wide range of diseases and ailments. ostrich antibodies against DHT and 5α-reductase have been produced and combined with antibodies against harmful skin bacteria to restore normal hair growth in AGA cases. Preliminary testing with six volunteer subjects showed that ostrich antibodies were clearly quite effective in hair regrowth.

关键词： Alopecia AGA ostrich Antibody

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基于RC缩放因子的IC物理设计时序一致性方法研究

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微电子学与计算机 2024年

作者：李锦明周焱江中北大学半导体与物理学院

在IC物理设计中，能够准确地对电路中的RC参数进行合理的提取计算，是对后面可以更快更高效完成时序收敛以及设计前后时序一致性的重要保障。本文针对数字IC物理设计中，由于不同工具对RC寄生参数提取的数值存在较大差异，而导致设计前... 详细信息

在IC物理设计中，能够准确地对电路中的RC参数进行合理的提取计算，是对后面可以更快更高效完成时序收敛以及设计前后时序一致性的重要保障。本文针对数字IC物理设计中，由于不同工具对RC寄生参数提取的数值存在较大差异，而导致设计前后时序一致性不能保证的问题，提出了一种采用辅助工具ostrich来对不同来源的SPEF文件进行比对，从而生成缩放因子对RC参数进行校准，将设计进行进一步修正。最后，通过项目验证，WNS和TNS与签核标准的契合度均有所提升，证明了该方法能够有效提高系统的可靠性并且实现设计前后时序一致性的目的。

关键词：物理设计时序一致性 RC参数 ostrich 缩放因子

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