检索结果-南通市图书馆

Chinese Journal of Oceanology and Limnology 2009年第4期27卷 723-728页

作者：丁森王芳董双林高勤峰 The Key Laboratory of Mariculture Ministry of EducationOcean University of China

The effects of various salinity fluctuation amplitudes （2, 4, 6 and 8） on the growth, osmolarity, Na＋-K＋-ATPase activity and Hsp70 of juvenile Fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studied. The results show that weight gain in the salinity fluctuation treatments was better than that in control; in particular, the weight gain of treatments S4 and SO, at 231.8% and 196.3%, respectively, was significantly different （P〈0.05）. The hemolymph osmolarity of treatments SO, S2, S4, S6 and S8 was 635.4, 630.8, 623.6, 614.4 and 600.3 mOsm/kg, respectively, and decreased with increasing salinity fluctuation amplitude. The level of Na＋-K＋-ATPase activity in gills ofE chinensis was higher than that in hepatopancreas, but there were no significant differences among all treatments, either in gills or hepatopancreas （P〉0.05）. The relative level of Hsp70 in treatment $4 was 48.4% and 40.4% higher than control in muscle and eyestalks, respectively, with the highest values being recorded under a salinity fluctuation amplitude of 4.

关键词： Fenneropenaeus chinensis growth osmolarity Na+-K+-ATPase activity Hsp70 salinityfluctuation amplitude

Effects of Salinity Fluctuation Frequency on the osmolarity,Na^+-K^+-ATPase Activity and HSP70 Expression in Juvenile Chinese Shrimp,Fenneropenaeus chinensis

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2009年第1期8卷 71-76页

作者： DING Sen WANG Fang SUN Hao GUO Biao DONG Shuanglin Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P. R. China National Marine Data Information Service Tianjin 300171 P. R. China

Experiments were conducted to examine the effects of salinity fluctuation frequency on the osmolarity, Na^＋-K^＋-ATPase activity and HSP70 of Chinese shrimp Fenneropenaeus chinensis ruth initial wet body weight of 1.460g±0.091 g. The salinity in the control group （DO） was 28 throughout the experiment, whereas treatments D2, D4, D6 and D8 were subjected to different salinity fluctuation frequencies of 2, 4, 6 and 8d, respectively. The salinity in treatments D2, D4, D6 and D8 was kept at 28 for 2, 4, 6 and 8d, respectively, decreased abruptly to salinity 24, lasted for another 2 d, and then was raised to its initial value 28. This was a complete salinity fluctuation cycle that afterwards repeated itself. After 32 days, the osmolarity in treatments D2, D4, D6 and D8 was significantly lower than that in treatment DO （P〈0.05）. There were significant differences in both muscle and eyestalks HSP70 expression among groups. The HSP70 expressions in muscle and eyestalks in group D4 were 61.4% and 57.0% higher, respectively, than that in the control group DO （P〈0.05）. There were, however, no significant differences in gill or hepatopancreas Na^＋-K^＋-ATPase activity between the treatments and the control.

关键词： Fenneropenaeus chinensis Salinity fluctuation frequency osmolarity Na^+-K^+-ATPase activity HSP70

The different osmolarity activates the astrocytes release different neurotransmitters signalling to the neurons induce different response in supraoptic nucleus of rat

在线全文

学校读者我要写书评

暂无评论

The different osmolarity activates the astrocytes release di...

Survival of rat sciatic nerve segments preserved in storage solutions ex vivo assessed by novel electrophysiological and morphological criteria

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Zhi-Ren RAO,Shan JIANG,Rong CAO,Li DUAN,Bei GAO,Ying-Fei XIONG and Xin-Hong WANG Institute of Neuroscience,The Fourth Military Medical University,Xi’an city,710032

The aim of this study is to investigate the response of astrocytes(AS) in rat supraoptic nucleus(SON) to different osmolar stimulation and the mechanism of affecting the activation of *** experiments were performed both in vivo and in *** adult male Sprague-Dawley rats were divided into two groups,(1) The model group:rats under anesthesia,0.15 M NaCl-(IS),1.5 M NaCl-(HS) and 0.83%glucose + 0.3%NaCl-(LS) solution(5.5 mL/kg B.W) was injected into the femoral vein(i.v.) respectively,as IS,HS and LS models.(2) The second group was pretreated with carbenoxolone(CBX,a gap junction blocker),or 6-aminomethyl-3-methyl-4H-1,2,4 -benzothiadiazine-1,1-dioxide(TAG,a taurine antagonist) or artificial cerebrospinal fluid(ACSF) containing Ca(2.5 mM).CBX,TAG or ACSF with Ca was injected into the lateral ventriculum,after 2 h performed IS,HS and LS *** 0 min before and 45min after IS,HS or LS ***,blood(5.5mL/kg,B.W.)was drew from the femoral veins of 5 rats in each group for radioimmunoassay of plasmic VP *** 90 min after injection(i.v.),all experimental rats were then sacrificed *** sections from hypothalamus were performed anti-vasopressin(VP),anti- N-methyl-D-aspartic acid receptor 2(NMDAR-2), anti-glycine receptor(GlyR) immunofluorescent staining and anti-glutamate(Glu)/ anti-glial fibrillary acidic protein(GFAP), anti-taurine(Tau)/anti-GFAP or anti-Fos/anti-GFAP double immunofluorescent *** sections were performed anti-connexin 43(Cx43)/NMDAR-2 or Cx43/GlyR double electromicroscopic *** results indicated that plasmic VP concentration of HS rats was higher significantly than that of IS,LS,CBX+HS,Ca+HS rats(P<0.001).Confocal microscopic image research showed that AS in HS rats were activated and revealed cellular hypertrophy,processes thickened,Fos-,GFAP- and Glu-expression up-regulating,and exhibited many Fos/GFAP-,or Glu/GFAP- double labeled *** Tau signal level was ***,NMDAR-2 and VP express

关键词： astrocyte supraoptic nucleus osmolarity glutamate taurine

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第9期18卷 2082-2088页

作者： Liwen Zhou Monzer Alatrach Ted Zhao Paul Oliphint George D.Bittner Department of Neuroscience University of Texas at AustinAustinTXUSA

Most organ or tissue allografts with viable cells are sto red in solutions ex vivo for hours to seve ral *** allografts then require rapid host revascula rization upon transplantation to maintain donor-cell functions(e.g.,cardiac muscle contra ctions,hepatic secretions).In contrast,peripheral nerve allografts stored ex vivo do not require revascularization to act as scaffolds to guide outgrowth by host axons at 1-2 mm/d,likely aided by viable donor Schwann *** current storage solutions and protocols,axons in all these donor orga n/tissue/nerve transplants are expected to rapidly become non-viable due to Wallerian degeneration within ***,ex vivo storage solutions have not been assessed for preserving normal axonal functions,i.e.,conducting action potentials or maintaining myelin *** hypothesized that most or all organ storage solutions would maintain axonal *** examined several common organ/tissue storage solutions(University of Wisconsin Cold Storage Solution,Normosol-R,Normal Saline,and La ctated Ringe rs) for axonal viability in rat sciatic nerves ex vivo as assessed by maintaining:(1) conduction of artificially-induced compound action potentials;and(2) axonal and myelin morphology in a novel assay *** ten diffe rent storage solution conditions for peripheral nerves with viable axons(PNVAs) diffe red in their solution composition,osmolarity(250-318 mOsm),temperature(4℃ vs.25℃),and presence of *** action potentials and axonal morphology in PNVAs were best maintained for up to 9 days ex vivo in calcium-free hypotonic diluted(250 mOsm) Normosol-R(dNR) at 4℃.Surprisingly,compound action potentials were maintained for only 1-2 days in UW and NS at 4℃,a much shorter duration than PNVAs maintained in 4℃ dNR(9 days) or even in 25℃ dNR(5 days).Viable axons in peripheral nerve allografts are critical for successful polyethylene glycol(PEG)-fusion of viable proximal and distal ends of host axons with viable donor a

关键词： axonal morphometrics axonal viability calcium osmolarity peripheral nerve grafts peripheral nerve injury temperature tissue storage solutions tonicity Wallerian degeneration

New approaches for diagnosis of dry eye disease

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2019年第10期12卷 1618-1628页

作者： Abdelrahman MElhusseiny Ali AKhalil Reem HEl Sheikh Mohammad ABakr MohamedGaber Eissa Yasmine MEl Sayed Department of Ophthalmology Kasr Al Ainy School ofmedicineCairo UniversityDokki12611Egypt Department of Ophthalmology Bascom Palmer Eye InstituteUniversity of Miami Miller School of Medicine900NW17StreetMiamiFL33136USA Faculty of Medicine American University of BeirutBeirut2341Lebanon

We reviewed the literature for different diagnostic approaches for dry eye disease(DED) including the most recent advances, contradictions and promising diagnostic tools and technique. We performed a broad literature search for articles discussing different methods for diagnosis of DED including assessment of tear osmolarity, tear film stability, ocular biomarkers and others. Articles indexed in PubMed and google scholar were included. With the growing cosmetic industry, environmental pollution, and booming of digital screens, DED is becoming more prevalent. Its multifactorial etiology renders the diagnosis challenging and invites the emergence of new diagnostic tools and tests. Diagnostic tools can be classified, based on the parameter they measure, into tear film osmolarity, functional visual acuity, tear volume, tear turnover, tear film stability, tear film composition, ocular biomarkers and others. Although numerous methods exist, the most accurate diagnosis can be reached through combining the results of more than one test. Many reported tests have shown potential as diagnostic/screening tools, however, require more research to prove their diagnostic power, alone or in combination. Future research should focus on identifying and measuring parameters that are the most specific to DED diagnosis.

关键词： dry eye disease tear film stability tear osmolarity ocular biomarkers

Osmotic pressure of serum and cerebrospinal fluid in patients with suspected neurological conditions

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2020年第5期15卷 944-947页

作者： Tetsuya Akaishi Toshiyuki Takahashi Ichiro Nakashima Michiaki Abe Masashi Aoki Tadashi Ishii Department of Education and Support for Regional Medicine Tohoku University HospitalSendaiJapan Department of Neurology Tohoku University Graduate School of MedicineSendaiJapan Department of Neurology National Hospital Organization Yonezawa National HospitalYonezawaJapan Department of Neurology Tohoku Medical and Pharmaceutical UniversitySendaiJapan

Interstitial fluid movement in the brain parenchyma has been suggested to contribute to sustaining the metabolism in brain parenchyma and maintaining the function of neurons and glial *** pulsatile hydrostatic pressure gradient may be one of the driving forces of this bulk ***,osmotic pressure- related factors have not been studied until *** this prospective observational study,to elucidate the relationship between osmolality (mOsm/kg) in the serum and that in the cerebrospinal fluid (CSF),we simultaneously measured the serum and CSF osmolality of 179 subjects with suspected neurological *** osmolality was 283.6 ± 6.5 mOsm/kg and CSF osmolality was 289.5 ± 6.6 mOsm/*** the specific gravity of serum and CSF is known to be 1.024–1.028 and 1.004–1.007,respectively,the estimated average of osmolarity (mOsm/L) in the serum and CSF covered exactly the same range (i.e.,290.5–291.5 mOsm/L).There was strong correlation between CSF osmolality and serum osmolality,but the difference in osmolality between serum and CSF was not correlated with serum osmolality,serum electrolyte levels,protein levels,or quotient of *** conclusion,CSF osmolarity was suggested to be equal to serum *** is not one of the driving forces of this bulk *** factors such as hydrostatic pressure gradient should be used to explain the mechanism of bulk flow in the brain *** study was approved by the Institutional Review Board of the Tohoku University Hospital (approval *** No.2015-1-257) on July 29,2015.

关键词： brain parenchyma bulk flow cerebrospinal fluid hydrostatic pressure interstitial fluid osmolarity osmotic pressure

Hypertonic and isotonic potassium solutions have different effects on vessel contractility resulting in differences in optimal resting tension in rat aorta

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第5期28卷 643-650页

作者： Yun-feng GUAN Ruo-hua CHEN Pei WANG Ye QIN Ding-feng SU Chao-yu MIAO Department of Pharmacology,Second Military Medical University,Shanghai 200433,China DepartmentofPharmacology SecondMilitaryMedicalUniversityShanghai200433China

Aim:To compare high K~+-induced contraction and optimal resting tension mea-sured by two commonly used techniques of hypertonic and isotonic K~+ in aortaswith and without adventitial fat from various age ***:Three age groupsof rats (15,25,and 62 weeks) were used to prepare thoracic aortic rings in whichadventitial fat was either removed or left *** K~+ (30 mmol/L)-inducedcontractions were observed under increasing resting tensions of 1.0,1.5,2.0,2.5,3.0,and 3.5 *** resting tension was the resting tension at which the aortashowed a maximal ***:The contractions induced by 2 kinds ofhigh K~+ were significantly *** and isotonic K~+ induced a differ-ent style of contraction,and the pattern varied with different *** the age of15 weeks,isotonic K~+-induced contractions were greater than hypertonic K~+-in-duced ***,at the age of 62 weeks,isotonic K~+-induced con-tractions were smaller than hypertonic K~+-induced *** restingtensions measured by 2 kinds of high K~+ were *** restingtensions in different kinds of aortic preparations from various age rats were almosta constant of 2 g,determined by isotonic K~+,but a variable,determined by hyper-tonic K~+.The adventitial fat could delay the development of high K~+-inducedcontractions at different resting tensions,but had little effect on the ***:Hypertonic and isotonic K~+ may produce differentcontractions resulting in differences in optimal resting tension in rat aorta.

关键词： potassium osmolarity contraction aorta fat

Disrupted ompC Causes Osmosis Sensitivity of Escherichia coli in Alkaline Medium

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2007年第12期34卷 1131-1138页

作者：王玉琪汪玲玲孙益嵘陈艺彩朱磊郭丽霞罗彪王海洪华南农业大学生命科学学院广州510642

The Escherichia coli strain DH42 is sensitive to high osmolarity in an alkaline medium. Using mini-Tn5 mutagenesis, construction of mutant strains by homologous recombination and subcloning of DNA fragment techniques, gene ompC was identified as the key factor that, once disrupted, caused osmosis-sensitivity of E. coli strain DH42 grown in an alkaline medium. Through P1 transduction, a mutant strain, D9 （W3110 ompC：kan）, was constructed and growth comparison was performed between DH42 and D9 under different pHs and salt concentrations. The result showed that ompC was necessarily required for hyperosmotic adaptation of E. coli in the alkaline medium.

关键词： ompC osmolarity alkaline pH

Shellfish allergy and relation to iodinated contrast media: United Kingdom survey

在线全文

学校读者我要写书评

暂无评论

World Journal of Cardiology 2014年第3期6卷 107-111页

作者： Mudassar Baig Ahmad Farag Jamal Sajid Rahul Potluri R Bruce Irwin Hafiz Mohammed Idrees Khalid Lancashire Cardiac Centre Blackpool Victoria HospitalBlackpoolLancashire FY3 8NRUnited Kingdom Cardiology Department Fairfield Hospital BuryPennine Acute TrustLancashire BL9 7TDUnited Kingdom ACALM Study Unit in partnership with School of Medical Sciences Aston UniversityBirmingham B4 7ETUnited Kingdom

AIM: To assess current practice of United Kingdom cardiologists with respect to patients with reported shellfish/iodine allergy, and in particular the use of iodinated contrast for elective coronary *** we have reviewed the current evidence-base and guidelines available in this ***: A questionnaire survey was send to 500senior United Kingdom cardiologists(almost 50% cardiologists registered with British Cardiovascular Society)using email and first 100 responses used to analyze practise. We involved cardiologists performing coronary angiograms routinely both at secondary and tertiary centres. Three specific questions relating to allergy were asked:(1) History of shellfish/iodine allergy in pre-angiography assessment;(2) Treatments offeredfor shellfish/iodine allergy individuals; and(3) Any specific treatment protocol for shellfish/iodine allergy cases. We aimed to establish routine practice in United Kingdom for patients undergoing elective coronary angiography. We also performed comprehensive PubMed search for the available evidence of relationship between shellfish/iodine allergy and contrast ***: A total of 100 responses were received, representing 20% of all United Kingdom cardiologists. Ninety-three replies were received from consultant cardiologists, 4 from non-consultant grades and 3 from cardiology specialist nurses. Amongst the respondents, 66% routinely asked about a previous history of shellfish/iodine allergy. Fifty-six percent would pre-treat these patients with steroids and anti-histamines. The other 44% do nothing, or do nonspecific testing based on their personal experience as following:(1) Skin test with 1 mL of subcutaneous contrast before intravenous contrast;(2) Test dose 2 mL contrast before coronary injection;(3) Close observation for shellfish allergy patients; and(4) Minimal evidence that the steroid and anti-histamine regime is effective but it makes us feel ***: There is no evidence that alle

关键词： Shellfish allergy Contrast allergy Iodinated contrast allergy Low osmolarity contrast media High osmolarity contrast media Pre-angiography assessment

From Russia with Polyuria

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Endocrine and Metabolic Diseases 2015年第5期5卷 51-53页

作者： DaLim Ki Udaya M. Kabadi Des Moines University Des Moines Iowa USA Veterans Affairs Medical Center Des Moines Iowa USA University of Iowa Iowa City Iowa USA

Introduction: Polyuria is a sign for many disease processes, including diabetes mellitus and diabetes insipidus. Urine osmolarity helps distinguish osmotic diuresis caused by diabetes mellitus from water dieresis induced by diabetes insipidus. Case Presentation: We report a case of a 48-year-old woman who presented with polyuria, polydipsia, nocturia, and weight loss after a return from a visit to Russia, during which she received a five-day course of antibiotic Demeclocycline, a tetracycline derivative for dental treatment. She recovered from all clinical manifestations by 8 weeks. Conclusion: Manifestation of transient nephrogenic diabetes insipidus is induced by Demeclocycline.

关键词： Polyuria Urine osmolarity Nephrogenic Diabetes Insipidus Demeclocycline