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Establishment of image-guided radiotherapy of orthotopic hepatocellular carcinoma mouse model

Animal models and Experimental Medicine 2023年第5期6卷 419-426页

作者： Kaixiao Zhou Yabo Jiang Shuang Feng Wei Mo Jing Nie Jianping Cao Yang Jiao School of Radiation Medicine and Protection State Key Laboratory of Radiation Medicine and ProtectionSoochow UniversitySuzhouChina The Six Department of Hepatic Surgery Eastern Hepatobiliary Surgery HospitalNavy Military Medical UniversityShanghaiChina Department of Radiotherapy Eastern Hepatobiliary Surgery HospitalNavy Military Medical UniversityShanghaiChina

Background:Hepatocellular carcinoma(HCC)is the most common type of liver ***,developments in radiotherapy technology have led to radiotherapy becoming one of the main therapeutics of ***,a suitable animal model for radiotherapy of the orthotopic HCC mouse model is urgently ***:In the present study,Hepa1-6 cells were injected into the liver of C57BL/6 mice in situ to mimic the pathological characteristics of the original *** formation was monitored by applying magnetic resonance imaging techniques and verified by H&E histopathological staining,AFP staining,and Ki67 staining.A single dose of 10 Gy X-ray was applied to simulate clinical radiotherapy plans using image-guided radiotherapy(IGRT)*** efficiency of radiotherapy was then assessed by examining tumor size and weight one week after ***-caspase3 staining and TUNEL were used to assess apoptosis in tumor ***:Intrahepatic tumor development was detected in the liver according using MRI.A high-density shadow could be seen 10 days after cell injection,which indicated the formation of HCC in *** tumors grew steadily bigger,and underwent precision radiotherapy 20 days after *** typical pathological characteristics of HCC,such as large,deeply stained nuclei and irregular cell size,were visible with H&E *** radiotherapy,significantly higher expression of the immunohistochemical markers Ki67 and AFP were detected in tumor tissue than in the nearby normal *** with the control group,the tumor volume(p=0.05)and weight(porthotopic HCC model,MRI was utilized to monitor the formation of tumors,and IGRT was used to simulate clinical *** present study could provide a suitable pre

关键词： hepatocellular carcinoma orthotopic model radiotherapy

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维普期刊数据库

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The role of mouse models in colorectal cancer research—The need and the importance of the orthotopic models

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Animal models and Experimental Medicine 2020年第1期3卷 1-8页

作者： Rui C.Oliveira Ana Margarida Abrantes JoseGuilherme Tralhao Maria Filomena Botelho Biophysics Unit Faculty of MedicineUniversity of CoimbraCoimbraPortugal Pathology Department University Hospital(CHUC)CoimbraPortugal Centre of Investigation on Environment Genetics and Oncobiology(CIMAGO)CoimbraPortugal Surgery A Department Faculty of MedicineUniversity Hospital(CHUC)CoimbraPortugal

Colorectal cancer is a worldwide health burden,with high incidence and mortality,especially in the advanced stages of the *** models are very important and valuable to discover and validate early and specific biomarkers as well as new therapeutic *** order to accomplish that,the animal models must replicate the clinical evolution of the disease in all of its *** this article,we review the existent mouse models,with their strengths and weaknesses in the replication of human cancer disease progression,with major focus on orthotopic models.

关键词： colorectal cancer mouse model orthotopic model

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Uncoupling protein 2 deficiency of non-cancerous tissues inhibits the progression of pancreatic cancer in mice

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Hepatobiliary & Pancreatic Diseases International 2023年第2期22卷 190-199页

作者： Denis Revskij Jakob Runst Camilla Umstätter Luise Ehlers Sarah Rohde Dietmar Zechner Manuela Bastian Brigitte Müller-Hilke Georg Fuellen Larissa Henze Hugo Murua Escobar Christian Junghanss Axel Kowald Uwe Walter Rüdiger Köhling Olaf Wolkenhauer Robert Jaster Department of Medicine Ⅱ Division of GastroenterologyRostock University Medical CenterRostockGermany Rudolf-Zenker-Institute of Experimental Surgery Rostock University Medical CenterRostockGermany Institute of Clinical Chemistry and Laboratory Medicine Rostock University Medical CenterRostockGermany Facility for Cell Sorting and Cell Analysis and Institute of Immunology Rostock University Medical CenterRostockGermany Institute for Biostatistics and Informatics in Medicine and Ageing Research Rostock University Medical CenterRostockGermany Department of Medicine Clinic ⅢHematologyOncologyPalliative MedicineRostock University Medical CenterRostockGermany Department of Neurology Rostock University Medical CenterRostockGermany Oscar LangendorffInstitute of Physiology Rostock University Medical CenterRostockGermany Department of Systems Biology and Bioinformatics University of RostockRostockGermany

Background: Pancreatic ductal adenocarcinoma(PDAC) is a disease of the elderly mostly because its development from preneoplastic lesions depends on the accumulation of gene mutations and epigenetic alterations over time. How aging of non-cancerous tissues of the host affects tumor progression, however, remains largely unknown. Methods: We took advantage of a model of accelerated aging, uncoupling protein 2-deficient( Ucp2 knockout, Ucp2 KO) mice, to investigate the growth of orthotopically transplanted Ucp2 wild-type(WT) PDAC cells(cell lines Panc02 and 6606PDA) in vivo and to study strain-dependent differences of the PDAC microenvironment. Results: Measurements of tumor weights and quantification of proliferating cells indicated a significant growth advantage of Panc02 and 6606PDA cells in WT mice compared to Ucp2 KO mice. In tumors in the knockout strain, higher levels of interferon-γ m RNA despite similar numbers of tumor-infiltrating T cells were observed. 6606PDA cells triggered a stronger stromal reaction in Ucp2 KO mice than in WT animals. Accordingly, pancreatic stellate cells from Ucp2 KO mice proliferated at a higher rate than cells of the WT strain when they were incubated with conditioned media from PDAC cells. Conclusions: Ucp2 modulates PDAC microenvironment in a way that favors tumor progression and implicates an altered stromal response as one of the underlying mechanisms.

关键词： Pancreatic cancer orthotopic model Uncoupling protein 2 Fibrosis

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维普期刊数据库

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Evaluation of direct intramural injection to the bladder wall as a method for developing orthotopic tumor models

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Animal models and Experimental Medicine 2022年第6期5卷 575-581页

作者： Masoud Bitaraf Samad Muhammadnejad Ashkan Azimzadeh Saman Behboodi Tanourlouee Erfan Amini Masoumeh Majidi Zolbin Abdol-Mohammad Kajbafzadeh Pediatric Urology and Regenerative Medicine Research Center GeneCell and Tissue Research InstituteTehran University of Medical SciencesTehranIran Gene Therapy Research Center Digestive Diseases Research InstituteTehran University of Medical SciencesTehranIran Uro-oncology Research Center Tehran University of Medical SciencesTehranIran

Background:Bladder cancer poses a great burden on society and its high rate of recurrence and treatment failure necessitates use of appropriate animal models to study its pathogenesis and test novel *** models are superior to other types since they provide a normal *** methods are described for developing bladder cancer models inside the animal’s *** intramural injection is one of these methods and is widely ***,its efficacy in model development has not yet been *** aimed to evaluate the efficacy and success rate of the direct intramural injection method of developing an orthotopic model for the study of bladder ***:Tumor cell lines were prepared in four *** of 200×10^(3) cells were injected through a 27 gauge needle into the ventral wall of the bladders of 4male and 4 female BALB/c mice following a midline 1 cm laparotomy *** addition,1 million cells from each microtube were injected into the flanks of control *** prevent infection and alleviate pain,5 mg/kg enrofloxacin and 2.5 mg/kg flunixin meglumine,respectively,were injected ***:Tumors formed in all mice,resulting in 100% take rate and zero post-operation *** time was≤15 min per *** two mice,tumors were found in the peritoneal space as ***:Direct intramural injection is a rapid,reliable,and reproducible method for developing orthotopic models of bladder *** can be done on both male and female mice and only requires readily available surgical ***,needle track can result in cell spillage and peritoneal tumors.

关键词： animal model bladder cancer bladder wall injection model evaluation orthotopic model

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维普期刊数据库

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