检索结果-南通市图书馆

In Vitro Propagation and Artificial Seed Production of Fritillaria cirrhosa D. Don, an Endangered Medicinal Plant

Phyton-International Journal of Experimental Botany 2024年第6期93卷 1297-1310页

作者： Qian Tao Guiqi Han Bujin Ma Hongmei Jia Can Zhao Wenshang Li Zhuyun Yan Chengdu University of Traditional Chinese Medicine State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest ChinaChengdu610000China

Fritillaria cirrhosa D.Don(Liliaceae)is an endangered perennial bulbous plant and its dry bulb is a valuable med-icinal material with antitussive and expectorant effects.Nevertheless,lack of resources and expensive prices make it difﬁcult to meet clinical needs.This study presents a regeneration system aimed at overcoming the challenge of inadequate supply in F.cirrhosa,focusing on:(1)callus induction,(2)bulblets and adventitious bud induction,and(3)artiﬁcial seed production.Callus development was achieved in 84.93%on Murashige and Skoog(MS)medium fortiﬁed with 1.0 mg·L^(-1) picloram.The optimal medium for callus differentiation into regenerated bulb-lets was MS medium supplemented with 1.0 mg·L^(-1)6-benzyladenine(6-BA)and 0.2 mg·L^(-1)α-naphthaleneacetic acid(NAA).Subsequently,bulblets and adventitious buds were induced from regenerated bulblet sections cul-tured on MS medium fortiﬁed with 0.3 mg·L^(-1)6-BA+1.0 mg·L^(-1)2,4-dichlorophenoxyacetic acid(2,4-D),2.0 mg·L^(-1)6-BA+0.5 mg·L^(-1),and the induction rates were 88.17%and 84.24%,respectively.The regenerated bulblets were transplanted into a substrate of humus soil,river sand,and pearlite(1:1:1)after low-temperature treatment.The germination rate was 42.80%after culture for 30 days.Regenerated bulblets were used for encap-sulations in liquid MS medium containing 3%sucrose(w/v)+0.5 mg·L^(-1) NAA+2.0 mg·L^(-1)6-BA+3%sodium alginate(w/v)with a 10 min exposure to 2%CaCl_(2).Under non-aseptic conditions,the germination rate reached 81.67%,while the rooting rate was 20.56%after 45 days.The capsule added 1.0 g·L^(-1) carbendazim and 1.0 g·L^(-1) activated carbon was the best component of artiﬁcial seeds.This study successfully established an efﬁcient regen-eration system for the rapid propagation of F.cirrhosa,involving in vitro bulblet regeneration and artiﬁcial seed production.This method introduces a novel approach for efﬁcient breeding and germplasm preservation,making it suitable for large-scale industrial resource production.

关键词： Artiﬁcial seed callus induction Fritillaria cirrhosa organogenesis plant propagation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The role of mesodermal signals during liver organogenesis in zebrafish

引用

Science China(Life Sciences) 2010年第4期53卷 455-461页

作者： NIU XuBo, SHI Hui & PENG JinRong College of Animal Sciences, Zhejiang University, Hangzhou 310029, China College of Animal Sciences Zhejiang University Hangzhou China

Three germ cell layers, the ectoderm, mesoderm and endoderm, are established during the gastrulation stage. All cell types in different organs and tissues are derived from these 3 germ cell layers at later stages. For example, skin epithelial cells and neuronal cells are derived from the ectoderm, while endothelial cells and muscle cells from the mesoderm and lung, and intestine epithelial cells from the endoderm. While in a normal situation different germ cells are destined to specific cell fates in different organs and tissues, each type of germ cells or its derivatives also produce extracellular signaling molecules to direct and facilitate the specification and differentiation of other germ cells during organogenesis. Liver is derived from the endoderm, but completion of liver organogenesis is regulated at different levels. While the pan-endoderm factors (e.g. FoxA and Gata families) and liver specific factors (e.g. Prox1 and Hhex) are essential intrinsic factors for endoderm cells to be differentiated into hepatoblasts, the role of signals produced by neighboring mesoderm cells for liver organogenesis is equally important. This review summarizes recent progress in studying the role of Bone morphogenetic proteins (Bmp), Fibroblast growth factors (Fgf), retinoic acid (RA) and Wingless and Int (Wnt), the 4 types of signaling molecules produced by the mesoderm cells, in liver organogenesis in zebrafish.

关键词： liver organogenesis zebrafish BMP FGF WNT

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Embryonic development and organogenesis of Chinese giant salamander,Andrias davidianus

引用

Progress in Natural Science:Materials International 2007年第11期17卷 1303-1311页

作者： Luo Jian, Xiao Yamei, Luo Kaikun, Huang Xiaoxi, Peng Liangyue, Liu Yun(Key Laboratory of Protein Chemistry and Fish Developmental Biology of National Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China) Key Laboratory of Protein Chemistry and Fish Developmental Biology of National College of Life Sciences Hunan Normal University Changsha 410081 China

The morphology and organogenesis of Chinese giant salamander, Andrias davidianus, in its different developmental periods and stages are described in detail, which provides an intact criterion for distinguishing different stages of its developmental process. Based on the external morphological and internal histological features, six periods including 20 stages of organogenesis of Chinese giant salamander are established, which are cleavage period, blastula period, gastrula period, neurula period, organogenesis stage and hatching stage. Generally, the embryonic development of Chinese giant salamander is consistent with those of Eastern newt, Cynops orientalis, and Black spots frog, R. nigromaculata. However, they have some differences in the early cleavage process and the development of digestive system. The cleavage of Chinese giant salamander, A. davidianus is not a discoidal division type, which is different from other species reported. And the first three cleavages being meridional and a retardant development of its digestive system without halter and sucker existing are the evident features of the embryonic development of Chinese giant salamander.

关键词： Chinese giant salamander Andrias davidianus embryogenesis cleavage organogenesis

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neuronal guidance genes in health and diseases

引用

Protein & Cell 2023年第4期14卷 238-261页

作者： Junichi Yuasa-Kawada Mariko Kinoshita-Kawada Yoshio Tsuboi Jane Y.Wu Department of Neurology Fukuoka University7.451 NanakumaJohnan.kuFukuoka 814-0180Japan Department of Neurology Center for Genetic MedicineLurie Cancer CenterNorthwestern University Feinberg School of MedicineWard Building Box 6117303E.Chicago AvenueChicagoIlinois 60611.USA

Neurons migrate from their birthplaces to the destinations,and extending axons navigate to their synaptic targets by sensing various extracellular cues in spatiotemporally controlled manners.These evolutionally conserved guidance cues and their receptors regulate multiple aspects of neural development to establish the highly complex nervous system by mediating both short-and long-range cell-cell communications.Neuronal guidance genes(encoding cues,receptors,or downstream signal transducers)are critical not only for development of the nervous system but also for synaptic maintenance,remodeling,and function in the adult brain.One emerging theme is the combinatorial and complementary functions of relatively limited classes of neuronal guidance genes in multiple processes,including neuronal migration,axonal guidance,synaptogenesis,and circuit formation.Importantly,neuronal guidance genes also regulate cell migration and cell-cell communications outside the nervous system.We are just beginning to understand how cells integrate multiple guidance and adhesion signaling inputs to determine overall cellular/subcellular behavior and how aberrant guidance signaling in various cell types contributes to diverse human diseases,ranging from developmental,neuropsychiatric,and neurodegenerative disorders to cancer metastasis.We review classic studies and recent advances in understanding signaling mechanisms of the guidance genes as well as their roles in human diseases.Furthermore,we discuss the remaining chalienges and therapeutic potentials of modulating neuronal guidance pathways in neural repair.

关键词： axon guidance neuronal migration synaptogenesis neural circuit formation neural mapping cell-cell communications angiogenesis organogenesis cancermetastasis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Micropropagation of loblolly pine by somatic organogenesis andRAPD analysis of regenerated plantlets

引用

Journal of Forestry Research 2000年第1期11卷 1-6页

作者：唐巍 O-23 E. S. King Village 2900 Ligon St. Forest Biotechnology Group North Carolina State University Raleigh NC 27607 USA

organogenesis was induced in callus derived from mature zygotic embryos of six families (J-56, S-1003, E-22, E-311, E-440, and Mc) of loblolly pine (Pinus taeda L.) within 24 weeks of culture. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg·L?1 indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). The most suitable medium for root formation proved to be TE medium supplemented with 0.5 mg·L?1 IBA, 2mg·L?1 BA and 0.5 mg/l gibberellic acid (GA3). 169 regenerated plantlets were transferred to a perlite: peatmoss: vermiculite (1∶1∶1) soil mixture, and 98 plantlets survived in the field. Total DNA was extracted from the needles of the regenerated plantlets of the six families of loblolly pine. Analysis of random amplified polymorphic DNA (RAPD) using 20 arbitrary oligonucleotide 10-mers, show that amplification products were monomorphic for all the plantlets of family J-56, S-1003, E-22, E-311, E-440, and Mc of loblolly pine. These results suggested that organogenesis can be used for clonal micropropagation of some families of loblolly pine.

关键词： Pinus taeda L. organogenesis Plant regeneration Random amplified polymorphic DNA (RAPD)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Endogenous auxin and its manipulation influence in vitro shoot organogenesis of citrus epicotyl explants

引用

Horticulture Research 2017年第1期4卷 26-31页

作者： Wei Hu Sabrina Fagundez Lorenzo Katin-Grazzini Yanjun Li Wei Li Yingnan Chen Xiaomin Wang Ziniu Deng Shenxi Xie Richard J McAvoy Yi Li National Center for Citrus Improvement Horticulture and Landscape CollegeHunan Agricultural UniversityChangsha410128People’s Republic of China Department of Plant Science and Landscape Architecture University of ConnecticutStorrs06269CTUSA Institute of Botany Jiangsu Province and Chinese Academy of SciencesNanjing210014People’s Republic of China

Endogenous auxin is an important regulator of in vivo organ development,but its role in in vitro organogenesis is unclear.It has been observed that the basal end of epicotyl cuttings of juvenile citrus seedlings produces fewer shoots than the apical end.Here,we report that elevated endogenous auxin levels in the basal end of citrus epicotyl cuttings are inhibitory for in vitro shoot organogenesis.Using transgenic citrus plants expressing an auxin-inducible GUS reporter gene,we have observed elevated levels of auxin at the basal end of stem cuttings that are mediated by polar auxin transport.Depleting endogenous auxin or blocking polar auxin transport enhances shoot organogenesis.An auxin transport inhibitor,N-1-naphthylphthalamic acid(NPA),can also enhance shoot organogenesis independent of its action on polar auxin transport.Finally,we demonstrate that the promotional effects of depleting endogenous auxin or blocking polar auxin transport on shoot organogenesis are cytokinin-dependent.Our study thus provides meaningful insights into possible roles of endogenous auxin and polar auxin transport,as well as auxin–cytokinin interactions,in in vitro shoot organogenesis.Meanwhile,our results may also provide practical strategies for improving in vitro shoot organogenesis for citrus and many other plant species.

关键词： organogenesis citrus endogenous

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MIKC^(C)-type MADS-box genes in Rosa chinensis: the remarkable expansion of ABCDE model genes and their roles in floral organogenesis

引用

Horticulture Research 2018年第1期5卷 617-631页

作者： Jinyi Liu Xiaodong Fu Yuwei Dong Jun Lu Min Ren Ningning Zhou Changquan Wang College of Horticulture Nanjing Agricultural UniversityNanjingJiangsu210095China Flower Research Institute Yunnan Academy of Agricultural SciencesKunmingYunnan650200China

MIKC^(C)-type MADS-box(MIKC^(C))genes encode transcription factors that have crucial roles in controlling floral organogenesis and flowering time in plants.Although this gene family has been well characterized in many plant species,its evolutionary and comprehensive functional analysis in rose is lacking.In this study,58 non-redundant MIKC^(C)uni-transcripts were extensively identified from rose transcriptomes.Phylogenetic analysis placed these genes into 12 clades with their Arabidopsis and strawberry counterparts,and revealed that ABCDE model(including AP1/FUL,AP3/PI,AG,and SEP clades),and SOC1 and AGL6 clade genes have remarkably expanded in Rosa chinensis,whereas genes from the FLC and AGL17 clades were undetectable.Sequence alignments suggest that the AP3/PI clade may contribute to more specific functions in rose due to a high variation of amino acid residues within its MADS-box domains.A comparative analysis of gene expression in specific floral organ differentiation stages and floral organs between R.chinensis cv.Old Blush and the closely related mutant genotype R.chinensis cv.Viridiflora(floral organs mutated into leaf-like structures)further revealed the roles of ABCDE model genes during floral organogenesis in rose.Analysis of co-expression networks provided an overview of the regulatory mechanisms of rose MIKC^(C)genes and shed light on both the prominent roles of AP3/PI clade genes in floral organogenesis and the roles of RcAGL19,RcAGL24,and RcSOC1 in regulating floral transition in rose.Our analyses provide an overall insight of MIKC^(C)genes in rose and their potential roles in floral organogenesis.

关键词： organogenesis organs floral

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tissue Culture of Calla Lily (Zantedeschia spreng.): An Updated Review on the Present Scenario and Future Prospects

引用

Phyton-International Journal of Experimental Botany 2023年第8期92卷 2413-2428页

作者： Xuan Sun Xue Wang Bijaya Sharma Subedi Yin Jiang Di Wang Rongxin Gou Guojun Zhang Wenting Xu Zunzheng Wei Institute of Grassland Flowers and EcologyBeijing Academy of Agriculture and Forestry SciencesBeijing100097China College of Horticultural Science&Technology Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative UtilizationHebei Normal University of Science&TechnologyQinhuangdao066004China Department of Agroecology and Plant Health The Robert H.Smith Faculty of AgricultureFood and EnvironmentThe Hebrew University of JerusalemRehovot7610001Israel Zhejiang Institute of Landscape Plants and Flowers Zhejiang Academy of Agricultural SciencesHangzhou311251China

The calla lily(Zantedeschia spreng.)is a bulbousﬂower native to the tropical regions of Africa.Calla lily has gained signiﬁcant popularity in the international market owing to its intricate morphology and prolonged flowering duration.Despite such advantages,for two sub-groups of calla lily,known as group Zantedeschia and group Aestivae,there are challenges in terms of hybrid production due to the‘plastome-genome incompatibility’there-between.Tissue culture is a fundamental biotechnological tool used in gene editing research,with a focus on disease resistance andﬂower color in calla lily breeding programs.The present review provides a brief background on the history and development of the calla lily,as well as a comprehensive and critical summary of calla lily tissue culture research.The regeneration pathways for both group Zantedeschia and group Aestivae can be divided into de novo organogenesis and somatic embryogenesis.Both groups are capable of obtaining replants through such means.However,only some species in group Aestivae have been reported to be successful in the somatic embryogenesis pathway.In the present review,special attention was paid to the inﬂuence of explant types,plant growth regulators,and culture conditions on both de novo organogenesis and somatic embryogenesis in calla lily tissue culture.Ultimately,future research prospects were determined based on integrated analysis of recent progress in calla lily tissue culture research.

关键词： Calla lily tissue culture organogenesis somatic embryogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In vitro Germination and Micropropagation of Aconitum vilmorinianum:An Important Medicinal Plant in China

引用

Phyton-International Journal of Experimental Botany 2022年第10期91卷 2331-2348页

作者： Zongmin Mou Fei Ye Fangchen Shen Dake Zhao School of Ecology and Environmental Science Yunnan UniversityKunming650504China Biocontrol Engineering Research Center of Plant Disease and Pest Yunnan UniversityKunming650504China Biocontrol Engineering Research Center of Crop Disease and Pest Yunnan UniversityKunming650504China Kunming Municipal Hospital of Traditional Chinese Medicine Kunming650500China

Aconitum vilmorinianum,a well-known traditional Chinese herb,is recently being threatened by overexploitation and environment disturbance.This study was conducted to provide propagation methods through in vitro germination and explant cultivation.Germination was stimulated up to 66.00%on Murashige and Skoog(MS)medium containing 2.0 mg L^(−1)6-benzylaminopurine(BAP),0.1 mg L^(−1)1-napthaleneacetic acid(NAA),and 30 g L^(−1)sucrose.Three bacteria(Pantoea agglomerans,Erwinia persicina,and Pseudomonas tolaasii)would be responsible for consistent contamination during germination.The latter two were effectively eradicated after disinfected.The influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated in the present work.The frequency of shoot induction from axillary bud explants was 100%on the MS fortified with 2.0 mg L^(−1)BAP and 0.3 mg L^(−1)NAA.Shoots multiplication was optimized on MS medium supplemented with 0.1 mg L^(−1)thidiazuron(TDZ)and 0.1 mg L^(−1)NAA.High callus induction percentage(96.67%)was obtained from stem segments on MS medium with 2.0 mg L^(−1)2,4-D,then successfully regenerated into shoots on MS medium in the presence of 0.1 mg L^(−1)TDZ and 0.2 mg L^(−1)NAA.The present work could be useful for the utilization and conservation of this valuable species.

关键词： Aconitum vilmorinianum seed surface bacteria in vitro multiplication organogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and organogenesis of Safflower (<i>Carthamus tinctorius</i>L.) cv. NARI-H-15

引用

American Journal of Plant Sciences 2017年第4期8卷 688-705页

作者： Jeyachandran Vijayakumar Ponnirul Ponmanickam P. Samuel D. N. P. Sudarmani J. Pandiarajan Department of Biotechnology Ayya Nadar Janaki Ammal College Sivakasi India Department of Zoology Ayya Nadar Janaki Ammal College Sivakasi India

The effect of meta-Topolin (mT) was assessed to develop a reliable protocol for efficient plant regeneration of safflower (Carthamus tinctorius L.) cv. NARI-H-15. For micropropagation, 7 - 9 days old shoot-tip explants cultured on MS basal medium supplemented with 3.0 mg/L meta-Topolin (mT) + 0.5 mg/L CPPU showed 97.7% adventitious shoot formation (42.4 shootlets) than node after 45 days of culture. For organogenesis, the seedling explants of immature leaf cultured on 1.5 mg/L CPPU or 1.5 mg/L NAA fortified medium produced high amount of callus than cotyledon and stem calli after 60 days of culture. However, MS basal medium fortified with 4.0 mg/L mT + 1.5 mg/L CPPU was found beneficial to stimulate 100% organogenic response (74.7 shootlets) from immature leaf calli than cotyledon and stem derived calli after 45 days of culture. The healthy plantlets obtained from micropropagation and organogenesis process cultured on 1/4 MS basal salts, 1.5% sucrose (w/v) and 0.8% agar (w/v) medium supplemented with NAA (1.5 mg/L) and mT (0.1 mg/L) produced maximum of 96% (12.8 rootlets) and 84% (7.3 rootlets) adventitious rooting, respectively than mT and CPPU tested medium. However, maximum of 67% and 42% survival rate was noticed when in vitro raised plants from micropropagation and organogenesis were hardened in pots containing soil mix and maintained under green house condition. This optimized regeneration protocol might be helpful in regeneration of new genotypes and cultivars of safflower to improve agronomic traits through in vitro selection process and Agrobacterium-mediated genetic transformation system.

关键词： Carthamus Tinctorius L. Meta-Topolin N-(2-chloro-4-pyridyl-N’-phenylurea) Micropropagation organogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：