Endogenous auxin and its manipulation influence in vitro shoot organogenesis of citrus epicotyl explants
作者机构:National Center for Citrus ImprovementHorticulture and Landscape CollegeHunan Agricultural UniversityChangsha410128People’s Republic of China Department of Plant Science and Landscape ArchitectureUniversity of ConnecticutStorrs06269CTUSA Institute of BotanyJiangsu Province and Chinese Academy of SciencesNanjing210014People’s Republic of China
出 版 物:《Horticulture Research》 (园艺研究(英文))
年 卷 期:2017年第4卷第1期
页 面:26-31页
核心收录:
学科分类:0710[理学-生物学] 071001[理学-植物学] 07[理学]
主 题:organogenesis citrus endogenous
摘 要:Endogenous auxin is an important regulator of in vivo organ development,but its role in in vitro organogenesis is *** has been observed that the basal end of epicotyl cuttings of juvenile citrus seedlings produces fewer shoots than the apical ***,we report that elevated endogenous auxin levels in the basal end of citrus epicotyl cuttings are inhibitory for in vitro shoot *** transgenic citrus plants expressing an auxin-inducible GUS reporter gene,we have observed elevated levels of auxin at the basal end of stem cuttings that are mediated by polar auxin *** endogenous auxin or blocking polar auxin transport enhances shoot *** auxin transport inhibitor,N-1-naphthylphthalamic acid(NPA),can also enhance shoot organogenesis independent of its action on polar auxin ***,we demonstrate that the promotional effects of depleting endogenous auxin or blocking polar auxin transport on shoot organogenesis are *** study thus provides meaningful insights into possible roles of endogenous auxin and polar auxin transport,as well as auxin–cytokinin interactions,in in vitro shoot ***,our results may also provide practical strategies for improving in vitro shoot organogenesis for citrus and many other plant species.