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Nonlinear optical imaging by detection with optical parametric amplification(invited paper)

Journal of Innovative optical Health Sciences 2023年第1期16卷 5-14页

作者： Yi Sun Haohua Tu Stephen A.Boppart Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign UrbanaIL 61801USA Department of Electrical and Computer Engineering University of Illinois at Urbana-Champaign UrbanaIL 61801USA Carle Illinois College of Medicine University of Illinois at Urbana-Champaign UrbanaIL 61801USA

Nonlinear optical imaging is a versatile tool that has been proven to be exceptionally useful in various research ***,due to the use of photomultiplier tubes(PMTs),the wide application of nonlinear optical imaging is limited by the incapability of imaging under am-bient *** this paper,we propose and demonstrate a new optical imaging detection method based on optical parametric amplification(OPA).As a nonlinear optical process,OPA in-trinsically rejects ambient light photons by coherence *** poled lithium niobate(PPLN)crystals are used in this study as the media for *** to bulk nonlinear optical crystals,PPLN crystals support the generation of OPA signal with lower pump ***,this characteristic of PPLN crystals is particularly beneficial when using high-repetition-rate lasers,which facilitate high-speed optical signal detection,such as in spec-troscopy and imaging.A PPLN-based OPA system was built to amplify the emitted imaging signal from second harmonic generation(SHG)and coherent anti-Stokes Raman scattering(CARS)microscopy imaging,and the amplified optical signal was strong enough to be detected by a biased photodiode under ordinary room light *** OPA detection,ambient-light-on SHG and CARS imaging becomes possible,and achieves a similar result as PMT detection under strictly dark *** results demonstrate that OPA can be used as a substitute for PMTs in nonlinear optical imaging to adapt it to various applications with *** ing conditions.

关键词： Nonlinear optical microscopy optical parametric amplification optical detection

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Real-time in situ detection and quantification of bacteria in the Arctic environment

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Journal of Innovative optical Health Sciences 2014年第2期7卷 35-45页

作者： Linda Powers Walther R.Ellis Jr. Christopher R.Lloyd Department of Electrical and Computer Engineering Department of Biomedical Engineering University of Arizona Tuc sonAZ 85721 Department of Biomedical Enginering University of ArizonaTucsonAz 85721 MicroBioSystems of Arizona 1665.E 18th St.Suite 204TucsonAZ 85719

At present,there are no methods that determine the total micro ial load on an abiotic substra tein real *** utility of such a capability ranges from sterilization and medical diagnostics tothe search for new microorganisms in the environment and study of their ecological *** the development of a hand held,fluorescence detection device and demonstrate its applicability to the field detection of Arctic bacteria,This technology is based on the early pioneering work of Britton Chance which elucidated the intrinsic fiuorescence of a number ofmetabolites and protein cofactors in cels,including reduced pyridine nucleotides,cytochromesand flavins.A PDA controls the device(fluorescence excitation and data collection)and processesthe multiwavelength signals to yield bacterial cell counts,including cstimates of ive cells,deadcells and *** existing methods for cell counting,this method requires no samplecontact or addition of *** use of this technology is demonstrated with in situmea surements of two sub-glacial microbial communities at sites in Palander and colonized surfacerocks in the Bockijord Volcanic Complex during AMASE 2008(Arctic Mars Analog Svalbard Expedition),The total bacterial load on the interrogated sample surfaces ranged from1o9 cells/cm^(2).

关键词： Intrinsic fuorescence microbial sensor optical detection amplitude modulation Svalbard Archipelago.

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A Microfluidic System with Active Mixing for Improved Real-Time Isothermal Amplification

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Journal of Beijing Institute of Technology 2022年第3期31卷 275-284页

作者： Dianlong Yang Xiaodan Jiang Yijie Zhou Xiaobin Dong Luyao Liu Lulu Zhang Xianbo Qiu College of Information Science and Technology Beijing University of Chemical TechnologyBeijing 100029China

To improve the performance of real-time recombinase polymerase amplification(RPA),a microfluidic system with active mixing is developed to optimize the reaction *** of adopting a single typical reaction chamber,a specific reactor including a relatively large chamber in center with two adjacent zig-zag channels at two sides is integrated into the microfluidic *** mixing is achieved by driving the viscous reagent between the chamber and the channel back and forth periodically with an outside compact peristaltic *** avoid reagent evapora-tion,one end of the reactor is sealed with paraffin oil.A hand-held companion device is developed to facilitate real-time RPA amplification within 20 *** whole area of the reactor is heated with a resistance heater to provide uniform reaction *** achieve real-time monitoring,a compact fluorescence detection module is integrated into the hand-held device.A smartphone with custom application software is adopted to control the hand-held device and display the real-time fluorescence *** performances of two cases with and without active on-chip mixing are compared between each other by detecting African swine fever *** has been demonstrated that,with active on-chip mixing,the amplification efficiency and detection sensitivity can be signifi-cantly improved.

关键词： recombinase polymerase amplification(RPA) microfluidic chip active mixing optical detection smartphone

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Germanium-on-silicon avalanche photodiode for 1550 nm weak light signal detection at room temperature

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Chinese Optics Letters 2022年第6期20卷 47-51页

作者： Yuxuan Li Xiaobin Liu Xuetong Li Lanxuan Zhang Baisong Chen Zihao Zhi Xueyan Li Guowei Zhang Peng Ye Guanzhong Huang Deyong He Wei Chen Fengli Gao Pengfei Guo Xianshu Luo Guoqiang Lo Junfeng Song State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin UniversityChangchun 130012China CAS Key Laboratory of Quantum Information University of Science and Technology of ChinaHefei 230026China CAS Center for Excellence in Quantum Information and Quantum Physics University of Science and Technology of ChinaHefei 230026China Advanced Micro Foundry Pte Ltd. Singapore 117685Singpore Peng Cheng Laboratory Shenzhen 518000China

To optimize the dark current characteristic and detection efficiency of the 1550 nm weak light signal at room temperature,this work proposes a Ge-on-Si avalanche photodiode[APD]in Geiger mode,which could operate at 300 *** lateral separate absorption charge multiplication APD shows a low breakdown voltage[V_(br)]in Geiger mode of-7.42 V and low dark current of 0.096 n A at unity gain voltage[V_(Gain)=1=-7.03 V].Combined with an RF amplifier module and counter,the detection system demonstrates a low dark count rate[DCR]of 1.1×10^(6) counts per second and high detection efficiencyηof 7.8%for 1550 nm weak coherent pulse detection at 300 *** APD reported in this work weakens the dependence of the weak optical signal recognition on the low environment temperature and makes single-chip integration of the single-photon level detection system possible.

关键词： avalanche photodiode optical detection optical interconnection

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Analysis of Nitrogen Dioxide in Environment

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Advances in Bioscience and Biotechnology 2016年第6期7卷 278-288页

作者： Jun Wang Wei Zhang Rui Cao Xiangyu You Hong Lai Department of Pharmacy Food and Pharmaceutical Engineering College Hubei University of Technology Wuhan Hubei China C. Eugene Bennett Department of Chemistry West Virginia University Morgantown WV USA Department of Chemistry Johns Hopkins University Baltimore Maryland USA Department of Radiology Johns Hopkins School of Medicine Baltimore Maryland USA

As a severe environmental pollutant, detection and quantitation of nitrogen dioxide (NO2) have been studied for centuries. In this review, recent progress of NO2 analysis in the atmosphere will be summarized. Four major types of detection technologies, including traditional chemical detection, optical detection, solid-state field effect transistor detection, and other detection technology are covered. The standard method employed by the US EPA, which is based on luminol, is the most reliable and robust method that is used for fully validated monitoring. In the past two decades, accompanying the fast development of electrical engineering and integrated circuit, micro to nanoscale gas sensors have been gaining more and more attention. Application of novel materials including nano wires and graphene also leads to a new era of research and development of sensors.

关键词： Nitrogen Dioxide Chemical detection optical detection Field-Effect Transistor detection Luminol

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Research of the light reflex surface defects detection technology

Research of the light reflex surface defects detection techn...

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2007亚洲光电子会议光学设计与测试分会场

作者： Jiajun Tian Yong Yao* Yunxu Sun Weijie Shi Xinhui Zhao Xuelian YU Department of Electronic and Information Engineering Shenzhen Graduate School Harbin Institute of Technology Shenzhen China 518055

The surface defects （indentations, humps, scores） of semiconductor wafers are the major factor to affect the performance of the semiconductor devices. Moreover, these defects make the manufacturing process difficult or yield of the semiconductor devices decrease. Consequently, it is necessary to do an on-line wafers detection in the benefit of the performance and yield of the devices. Thus, developing a way suited for practical detection is the urgent affair faced to people. The optical methods are the most suited ways for surface defects detection because of their non-contact operation. We discuss a relatively unknown method of the light reflex surface defects detection technology （Makyoh （or magic-mirror） topography （MT））. The optical system that we designed achieves the detection mode of the parallel light vertical incidence for the first time. It corrects the deviation between the tradition light path and arithmetic, promotes the detection precision. A lot of experiments based on this optical system are taken to get the difference between the detection modes of vertical incidence and the oblique incidence. The experiments prove that the vertical incidence mode excels the oblique incidence mode. The MT images are also analyzed and disposed. The quantitative measurement can be realized by digital image processing technology.

关键词： optical detection surface defects Makyoh (magic-mirror) topography(MT) quantitative detection

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