A Microfluidic System with Active Mixing for Improved Real-Time Isothermal Amplification

作     者：Dianlong Yang Xiaodan Jiang Yijie Zhou Xiaobin Dong Luyao Liu Lulu Zhang Xianbo Qiu Dianlong Yang;Xiaodan Jiang;Yijie Zhou;Xiaobin Dong;Luyao Liu;Lulu Zhang;Xianbo Qiu

作者机构：College of Information Science and TechnologyBeijing University of Chemical TechnologyBeijing 100029China 

出 版 物：《Journal of Beijing Institute of Technology》 (北京理工大学学报（英文版）)

年 卷 期：2022年第31卷第3期

页      面：275-284页

核心收录：

学科分类：080903[工学-微电子学与固体电子学] 0810[工学-信息与通信工程] 081704[工学-应用化学] 07[理学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by the National Natural Science Foundation of China(Nos.81871505,61971026) the Fundamental Research Fund for the Central Universities(No.XK1802-4) the National Science and Technology Major Project(No.2018ZX10732101-001-009) the Research Fund to the Top Scientific and Technological Innovation Team from Beijing University of Chemical Technology(No.buctylkjcx06) 

主　　题：recombinase polymerase amplification(RPA) microfluidic chip active mixing optical detection smartphone 

摘      要：To improve the performance of real-time recombinase polymerase amplification(RPA),a microfluidic system with active mixing is developed to optimize the reaction *** of adopting a single typical reaction chamber,a specific reactor including a relatively large chamber in center with two adjacent zig-zag channels at two sides is integrated into the microfluidic *** mixing is achieved by driving the viscous reagent between the chamber and the channel back and forth periodically with an outside compact peristaltic *** avoid reagent evapora-tion,one end of the reactor is sealed with paraffin oil.A hand-held companion device is developed to facilitate real-time RPA amplification within 20 *** whole area of the reactor is heated with a resistance heater to provide uniform reaction *** achieve real-time monitoring,a compact fluorescence detection module is integrated into the hand-held device.A smartphone with custom application software is adopted to control the hand-held device and display the real-time fluorescence *** performances of two cases with and without active on-chip mixing are compared between each other by detecting African swine fever *** has been demonstrated that,with active on-chip mixing,the amplification efficiency and detection sensitivity can be signifi-cantly improved.