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Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids:the potential association with oocyte quality

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1727-1742页

作者： Ahmed Gad Matej Murin Alexandra Bartkova Veronika Kinterova Katerina Marcollova Jozef Laurincik Radek Prochazka Institute of Animal Physiology and Genetics Czech Academy of Sciences27721 LiběchovCzech Republic Department of Animal Production Faculty of AgricultureCairo UniversityGiza 12613Egypt Department of Botany and Genetics Faculty of Natural SciencesConstantine the Philosopher University in Nitra94901 NitraSlovakia.

Background: Ovarian follicular fluids(FFs) contain several kinds of regulatory factors that maintain a suitable micro-environment for oocyte development. Extracellular vesicles(EVs) are among the factors that play essential roles in regulating follicle and oocyte development through their cargo molecules that include microRNAs(miRNAs). This study aimed to investigate small-EV(s-EV) miRNAs in porcine FFs and their potential association with oocyte ***: Individual aspirated oocytes were stained with lissamine green B stain(LB), a vital stain for oocyte quality, and each oocyte was classified as high-quality(unstained;HQ) or low-quality(stained;LQ). FFs corresponding to oocytes were pooled together into HQ and LQ groups. Small-EVs were isolated from FFs, characterized, and their miRNA cargo was identified using the Illumina Nova Seq sequencing platform. Additionally, s-EVs from the HQ and LQ groups were utilized to investigate their effect on oocyte development after co-incubation during in vitro ***: A total of 19 miRNAs(including miR-125b, miR-193a-5p, and miR-320) were significantly upregulated, while 23(including miR-9, miR-206, and miR-6516) were downregulated in the HQ compared to the LQ group. Apoptosis, p53 signaling, and cAMP signaling were among the top pathways targeted by the elevated miRNAs in the HQ group while oocyte meiosis, gap junction, and TGF-beta signaling were among the top pathways targeted by the elevated miRNAs in the LQ group. The supplementation of small-EVs during maturation does not affect the oocyte developmental rates. However, LQ s-EVs increase the proportion of oocytes with homogeneous mitochondrial distribution and decrease the proportion of heterogeneous ***: Our findings indicated that FF-EVs contain different miRNA cargos associated with oocyte quality and could affect the mitochondrial distribution patterns during oocyte maturation.

关键词： Extracellular vesicles Follicular fuids MiRNA oocyte quality Porcine

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Markers of oocyte quality to Enhance Human IVF Outcomes: A Bibliographic Review

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Open Journal of Internal Medicine 2024年第1期14卷 102-121页

作者： Muhammad Kayanja Moses Tiri Mark Muyigo Davis Rujumba Nyiro Brian Kalule Francis Neogenesis Fertility Centre Kampala Uganda IVI Valencia Valecia Spain College of Health Sciences Department of Immunology and Molecular Biology Makerere University Makerere Uganda Zoetis ALPHA Plus Initiative Zaventum Belgium Faculty of Science and Technology University of Nairobi Nairobi Kenya

The markers of oocyte quality have remained a major controversy in the field of embryology due to the subjectivity of the different methods of oocyte assessment. Various scholars use oocyte quality and oocyte competence interchangeably. oocyte quality can be defined as the overall health of an oocyte whereas oocyte competence refers to the ability of an oocyte to be fertilized and develop into a healthy embryo. Diminished oocyte quality is believed to be a result of alterations in oocyte growth and maturation processes that stem from several pelvic and systemic factors before and after oocyte retrieval. In this review, we focus on the morphological and nonmorphological markers of oocyte quality. Strict restrictions that limit the number of oocytes fertilized in various countries have triggered researchers around the world to come up with the most appropriate and noninvasive markers that enhance oocyte selection and optimize IVF outcomes. PubMed, Google Scholar, and the Cochrane Library were used to search for peer-reviewed, original articles about oocyte quality markers. The review was written in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Morphological markers are commonly used, but they are subjective, and no single marker can be used exclusively to predict oocyte competence and subsequent embryonic development potential. Furthermore, transcriptomics of differentially expressed genes in cumulus cells and assessment of metabolomics and other contents of follicular fluid have shown greater precision. However, their specificity to the different quality determinants needs further research.

关键词： oocyte quality oocyte Competence Metabolomics Transcriptomics oocyte Morphology

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Mitochondrial Dysfunction and Age-related oocyte quality

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Reproductive and Developmental Medicine 2017年第1期1卷 45-54页

作者： Han Li Ri-Cheng Chian Center for Reproductive Medicine the 10th People’s Hospital of Tongjin UniversityShanghai 200072China Department of Obstetrics and Gynecology McGill UniversityMontrealCanada

Fertility disorders have become a growing problem worldwide. It is well known that female fertility decreases with age, previous studies suggested that the age-related decline in female fertility potential was largely due to decrease in oocyte quality and mitochondrial dysfunction. Mitochondria play a crucial role during the process of oocyte maturation. Mitochondrial genetic, numerical and structural defects occur in oocyte aging process, mitochondrial abnormalities are believed to contribute to age-related infertility. Improvement of the mitochondrial function can lead to better fertility outcomes, and application of mitochondria replacement strategy or mitochondrial transfer to age-related infertility will be possible in the future. This review paper, we are trying to discuss current understanding about age-related changes in oocyte quality and mitochondrial dysfunction.

关键词： Mitochondrial Dysfunction oocyte quality Therapeutic Strategies

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Endogenous biosynthesis of docosahexaenoic acid(DHA)regulates fish oocyte maturation by promoting pregnenolone production

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Zoological Research 2024年第1期45卷 176-188页

作者： Yi Li Xuehui Li Ding Ye Ru Zhang Chengjie Liu Mudan He Houpeng Wang Wei Hu Yonghua Sun State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyInnovation Academy for Seed DesignChinese Academy of SciencesWuhanHubei 430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Hubei Hongshan Laboratory WuhanHubei 430070China

Omega-3 polyunsaturated fatty acids(n-3 PUFAs),particularly docosahexaenoic acid(22:6n-3,DHA),play crucial roles in the reproductive health of vertebrates,including ***,the underlying mechanism related to this phenomenon remains largely *** this study,we employed two zebrafish genetic models,i.e.,elovl2^(-/-)mutant as an endogenous DHAdeficient model and fat1(omega-3 desaturase encoding gene)transgenic zebrafish as an endogenous DHA-rich model,to investigate the effects of DHA on oocyte maturation and *** show that the elovl2^(-/-)mutants had much lower fecundity and poorer oocyte quality than the wild-type controls,while the fat1 zebrafish had higher fecundity and better oocyte quality than wildtype *** deficiency in elovl2^(-/-)embryos led to defects in egg activation,poor microtubule stability,and reduced pregnenolone *** study revealed that DHA promoted pregnenolone synthesis by enhancing transcription of cyp11a1,which encodes the cholesterol side-chain cleavage enzyme,thereby stabilizing microtubule assembly during *** turn,the hypothalamic-pituitary-gonadal axis was enhanced by *** conclusion,using two unique genetic models,our findings demonstrate that endogenously synthesized DHA promotes oocyte maturation and quality by promoting pregnenolone production via transcriptional regulation of cyp11a1.

关键词： Docosahexaenoic acid oocyte maturation oocyte quality Pregnenolone Microtubule

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Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1326-1339页

作者： Meijie Song Yu Li Yihang Zhou Jiner Yan Xiaohua Zhou Qian Gao Yilong Miao Bo Xiong College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China College of Veterinary Medicine Nanjing Agricultural UniversityNanjing 210095China

Background:Elevated ambient temperature-caused heat stress is a major concern for livestock production due to its negative impact on animal feed intake,growth,reproduction,and ***,the germ cells are extremely sensitive to the heat ***,the effective approach and strategy regarding how to protect mammalian oocytes from heat stress-induced defects have not been ***:Germinal vesicle(GV)porcine oocytes were cultured at 41.5℃ for 24 h to induce heat stress,and then cultured at 38.5℃ to the specific developmental stage for subsequent *** mononucleotide(NMN)was dissolved in water to 1 mol/L for a stock solution and further diluted with the maturation medium to the final concentrations of 10μmol/L,20μmol/L,50μmol/L or 100μmol/L,respectively,during heat *** and fluorescence intensity quantification were applied to assess the effects of heat stress and NMN supplementation on the key processes during the oocyte meiotic ***:Here,we report that NMN supplementation improves the quality of porcine oocytes under heat ***,we found that heat stress resulted in oocyte maturation failure by disturbing the dynamics of meiotic organelles,including the cytoskeleton assembly,cortical granule distribution and mitochondrial *** addition,heat stress induced the production of excessive reactive oxygen species(ROS)and DNA damage,leading to the occurrence of apoptosis in oocytes and subsequent embryonic development *** importantly,we validated that supplementation of NMN during heat stress restored the meiotic defects during porcine oocyte ***:Taken together,our study documents that NMN supplementation is an effective approach to improve the quality of oocytes under heat stress by promoting both nuclear and cytoplasmic maturation.

关键词： Heat stress Meiotic defects Nicotinamide mononucleotide oocyte quality Porcine oocytes

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Ovarian aging:mechanisms and intervention strategies

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Medical Review 2022年第6期2卷 590-610页

作者： Zhengmao Zhu Wanxue Xu Lin Liu Haihe Laboratory of Cell Ecosystem Chinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China Department of Genetics and Cell Biology College of Life ScienceNankai University94 Weijing RoadTianjin 300071China Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijingChina Tianjin Union Medical Center Institute of Translational MedicineNankai UniversityTianjin 300000China

Ovarian reserve is essential for fertility and influences healthy aging in *** maternal age correlates with the progressive loss of both the quantity and quality of *** molecular mechanisms and various contributing factors underlying ovarian aging have been *** this review,we highlight some of critical factors that impact oocyte quantity and quality during *** cell and follicle reserve at birth de-termines reproductive lifespan and timing the menopause in female *** diminishing ovarian reserve leads to premature ovarian aging or *** oocyte quality with increasing age could result from chromosomal cohesion deterioration and misaligned chromosomes,telomere shortening,DNA damage and associated genetic mutations,oxidative stress,mitochondrial dysfunction and epigenetic *** also discuss the intervention strategies to delay ovarian *** the efficacy of senotherapies by antioxidants against reproductive aging and mitochondrial therapy are *** oocytes and ovarioids could be rejuvenated from pluripotent stem cells or somatic *** propose directions for future *** couples increasingly begin delaying parenthood in life worldwide,understanding the molecular mechanisms during female reproductive aging and potential intervention strategies could benefit women in making earlier choices about their reproductive health.

关键词： mitochondrial therapy oocyte quality oocyte quantity ovarian aging ovarioids senotherapy

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Recent progress in the treatment of women with diminished ovarian reserve

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Gynecology and Obstetrics Clinical Medicine 2021年第4期1卷 186-189页

作者： Jingwen Yin Hsun-Ming Chang Rong Li Peter C.K.Leung Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalChina National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital) China Key Laboratory of Assisted Reproduction(Peking University) Ministry of EducationChina Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology China Department of Obstetrics and Gynelogy University of British ColumbiaAnd BC Children's Hospital Research InstituteVancouverBritish ColumbiaV5Z 4H4Canada

Diminished ovarian reserve(DOR)refers to a decrease in the number and/or quality of oocytes in the ovary,accompanied by a decline in reproductive potential,which is generally related to advanced age or ovarian *** in vitro fertilization(IVF)clinical practice,managing patients with DOR remains one of the most challenging *** recent years,increased research on improving ovarian function has provided us with new insights into treating patients with *** therapeutic options have been proposed to improve the ovarian function of patients with DOR,yet they are not widely utilized in clinical practice because of limited evidence of safety and *** this review,we focus on the mechanisms from animal models and clinical trials that have been applied to the treatment of DOR in recent years,intending to improve IVF outcomes in patients with ***,new insights and perspectives on the molecular and cellular regulation of follicular development and ovarian reserve are emphasized to provide more clues for research on the treatment of DOR.

关键词： Diminished ovarian reserve Infertility In vitro fertilization Ovarian function oocyte quality Embryo quality

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Expression of Target Genes in Cumulus Cells Derived from Human oocytes with and without Blastocyst Formation

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Reproductive and Developmental Medicine 2019年第2期3卷 84-88页

作者： Tuan-Ping Zhou Di Zhang Ling-Bo Cai Yi-Xin Xu Li Shao Kai-Lu Liu Jia-Yin Liu Yu-Gui Cui Ling Wang Ri-Cheng Chian State Key Laboratory of Reproductive Medicine Clinical Center for Reproductive MedicineThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 211166China Center for Reproductive Medicine Shanghai 10th People’s Hospital of Tongji UniversityShanghai 200072China

Objective:The transcriptional profile of cumulus cells(CCs)during oocyte maturation provides information for predicting oocyte developmental *** previous study using a mouse model indicated that there were nine different genes related to oocyte development potential expressed in CCs during oocyte *** purpose of this study was to elucidate whether gene expression levels of CCs during oocyte maturation are associated with oocyte developmental ***:The human CCs collected from each oocyte were divided into two groups after tracking depending on whether or not they developed to the blastocyst stage:(1)the oocytes were developed to blastocyst stage after fertilization(B+)and(2)the oocytes were not developed to blastocyst stage after fertilization(B−).The expression levels of the nine selected genes(ARRB1,ATP2C1,CDH5,CNTNAP1,LGR4,MKLN1,RHOBTB1,SIX2,and SMC2)were *** were obtained from 29 women who were undergoing intracytoplasmic sperm injection treatment *** reverse transcriptase-polymerase chain reaction analysis was performed on cumulus masses collected before *** sample was run three *** significant differences in mRNA expression of the target genes in independent samples were evaluated by two-tailed Student’s t-test,and Poocyte developmental competence before insemination and fertilization.

关键词： Cumulus Cells Developmental Competence In vitro Maturation oocyte quality oocyte

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Correlation between the expression of GSTT1 in ovarian granulosa cells and IVF outcome

Correlation between the expression of GSTT1 in ovarian granu...

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2013浙江省医学遗传学学术年会

作者： Zhangfan Cai yili Yuji Takahashi Jin Fan Department of Reproductive Endocrinology Key Laboratory of Reproductive GeneticsMinistry of Educationand Women's HospitalZhejiang University School of Medicine Department of Reproductive Medicine The Affiliated Wuxi Hospital for Maternal and Children Health Care of Nanjing Medical University

Background We previously reported that Glutathione S-transferase theta 1(GSTTl) was highly up-regulated in aged human ovarian granulosa cells,suggesting that GSTTl may be a biomarker for oocyte quality in age-related *** purpose of this study was to investigate the correlations between the expression level of GSTTl in granulosa cells and the IVF *** Granulosa cells were obtained from seventy-two infertility women who underwent in vitro fertilization-embryo transfer(IVF-ET) between August and December *** RNA was isolated from mural granulosa cells,and the expression level of GSTTl gene was analyzed by the real-time *** Correlation between the expression level of GSTTl and IVF outcome was *** We separated the patients into two groups according to the expression level of *** compared to low GSTTl expression group,the ratio of mature oocytes (MII) and fertilization were significantly lower in the high GSTTl expression group(poocytes and *** may be a novel candidate marker for indicating the outcomes of IVF.

关键词： GSTT1 granulosa cell oocyte quality infertility IVF outcomes

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酒精对小鼠颗粒细胞功能的影响（英文）

酒精对小鼠颗粒细胞功能的影响（英文）

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陕西省毒理学会新时代环境与健康学术会议暨第四届会员代表大会

作者： Wanlu Xu Lihui Huo Jingjing Li Chunli Xu Shuang Wang Yanhong Yang Chuang Liu Xiaomin Zheng Xiuliang Feng Xingrong Yan Shaanxi Key Laboratory for Animal Conservation Key Laboratory of Resource Biology and Biotechnology in Western ChinaMinistry of EducationCollege of Life ScienceNorthwest University Department of Experimental Surgery of Xijing Hospital the Fourth Military Medical University Department of Obstetrics & Gynecology Tangdu Hospitalthe Fourth Military Medical University Key Laboratory of Fertility Preservation and Maintenance Ministry of Education Institute of Biomedicine PharmacologyBiomedicum HelsinkiUniversity of Helsinki

Alcohol is an important compound used in food,agriculture,and *** this study we investigated the effect of alcohol on oocyte quality in mice by exposing animals for different duration times during an estrous ***-oocyte complexes(COCs) were collected from mice following pregnant mare serum gonadotropin(PMSG)-and human chorionic gonadotropin(hCG)-induced *** number,E level in serum,and parthenogenetic embryo development in vitro were *** gene expression,mitochondrial membrane potential,and reactive oxygen species(ROS) levels in the cumulus were also *** results showed that acute exposure to alcohol did not affect ovulation time(p> 0.05).Blasocyst formation rate in vitro was significantly improved after 1 and 2 days of alcohol exposure(p oocyte quality by influencing the function and gene expression in the *** results underscore potential implications for the development of human reproductive therapeutics.

关键词： oocyte quality alcohol mitochondria mice parthenogenesis

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