Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

作     者：Meijie Song Yu Li Yihang Zhou Jiner Yan Xiaohua Zhou Qian Gao Yilong Miao Bo Xiong Meijie Song;Yu Li;Yihang Zhou;Jiner Yan;Xiaohua Zhou;Qian Gao;Yilong Miao;Bo Xiong

作者机构：College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2022年第13卷第5期

页      面：1326-1339页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31900592) the Natural Science Foundation of Jiangsu Province(BK20190526) 

主　　题：Heat stress Meiotic defects Nicotinamide mononucleotide Oocyte quality Porcine oocytes 

摘      要：Background:Elevated ambient temperature-caused heat stress is a major concern for livestock production due to its negative impact on animal feed intake,growth,reproduction,and ***,the germ cells are extremely sensitive to the heat ***,the effective approach and strategy regarding how to protect mammalian oocytes from heat stress-induced defects have not been ***:Germinal vesicle(GV)porcine oocytes were cultured at 41.5℃ for 24 h to induce heat stress,and then cultured at 38.5℃ to the specific developmental stage for subsequent *** mononucleotide(NMN)was dissolved in water to 1 mol/L for a stock solution and further diluted with the maturation medium to the final concentrations of 10μmol/L,20μmol/L,50μmol/L or 100μmol/L,respectively,during heat *** and fluorescence intensity quantification were applied to assess the effects of heat stress and NMN supplementation on the key processes during the oocyte meiotic ***:Here,we report that NMN supplementation improves the quality of porcine oocytes under heat ***,we found that heat stress resulted in oocyte maturation failure by disturbing the dynamics of meiotic organelles,including the cytoskeleton assembly,cortical granule distribution and mitochondrial *** addition,heat stress induced the production of excessive reactive oxygen species(ROS)and DNA damage,leading to the occurrence of apoptosis in oocytes and subsequent embryonic development *** importantly,we validated that supplementation of NMN during heat stress restored the meiotic defects during porcine oocyte ***:Taken together,our study documents that NMN supplementation is an effective approach to improve the quality of oocytes under heat stress by promoting both nuclear and cytoplasmic maturation.