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The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean

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Plant Diversity 2023年第4期45卷 469-478页

作者： Qing Tan Bing Han Mohammad Enamul Haque Ye-Lan Li Yue Wang Di Wu Shi-Bo Wu Ai-Zhong Liu Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650204China Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China(Ministry of Education) Southwest Forestry UniversityKunming 650224China College of Life Sciences Shaanxi Normal UniversityXi'an 710119China University of Chinese Academy of Sciences Beijing 100101China

The transcription factor WRINKLED1(WRI1),a member of AP2 gene family that contain typical AP2 domains,has been considered as a master regulator regulating oil biosynthesis in ***,the regulatory mechanism of RcWRI1 in regulating oil accumulation during seed development has not been clearly *** bean(Ricinus communis)is one of the most important non-edible oil crops and its seed oils are rich in hydroxy fatty acids,widely applied in *** this study,based on castor bean reference genome,three RcWRIs genes(RcWRI1,RcWRI2 and RcWRI3)were identified and the expressed association of RcWRI1 with oil accumulation were *** transformation of RcWRI1 significantly increased oil content in tobacco leaf,confirming that RcWRI1 activate lipid biosynthesis *** DNA Affinity Purification sequencing(DAP-seq)technology,we confirmed RcWRI1 binding with Transcription Start Site of genes and identified 7961 WRI1-binding candidate ***,these identified genes were mainly involved in diverse metabolism pathways(including lipid biosynthesis).Three cis-elements AW-box([CnTnG](n)7[CG])and AW-boxes like([GnAnC](n)6[GC]/[GnAnC](n)7[G])bound with RcWRI1 were ***-expression network analysis of RcWRI1 further found that RcWRI1 might be widely involved in biosynthesis of storage materials during seed *** particular,yeast one hybrid experiments found that both AP2 domains within RcWRI1 were required in binding targeted *** results not only provide new evidence to understand the regulatory mechanism of RcWRI1 in regulation of oil accumulation during castor bean seed development,but also give candidate gene resource for subsequent genetic improvement toward increasing oil content in oilseed crops.

关键词： Castor bean WRI transcription factor oil accumulation Developing seeds Lipid gene

Effects of Heat Stress during Seed Filling Stage on Brassica napus Seed oil accumulation and Chlorophyll Fluorescence Characteristics

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 333-348页

作者： Ruizhi Huang Huasheng Yu Yong Yang Heqin Liu Xuelong Wu Zhihong Liu Haiyan He Gengwei Wu Wengjia Wang Hua Wang State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Institute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhou310021China Institute of Crop Science and Nuclear Technology Utilization Zhejiang Academy of Agricultural SciencesHangzhou310021China

As global temperature rise,the threat of heat stress to rapeseed production is becoming more *** the response characteristics of two important biological pathways,oil accumulation and photosynthesis,to heat stress during *** seed filling is helpful in the genetic improvement of heat-tolerant *** effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 *** germplasms with different oil content and environmental sensitivity,including 6 rapeseed varieties which exhibited environmentsensitive/insensitive and with high,medium or low oil content,were tested by whole plant heat stress or the in vitro silique culture *** assay exhibited similar trend on oil content of the rapeseed *** heat effect on the chlorophyll fluorescence kinetic parameters F_(v)/F_(m),ETR and Y(Ⅱ)were also *** stress significantly decreased oil content,although there was abundant genetic variation on heat tolerance among the *** analysis showed that the decrease rate of F_(v)/F_(m) of silique heat-stressed *** developing seed was positive correlative to the decrease rate of mature seed oil content of the whole plant heat-stressed rapeseed(R=0.9214,P-valueoil accumulation and photosynthesis in *** developing *** decrease rate of chlorophyll fluorescence parameter F_(v)/F_(m) of heat-stressed developing seed could be used as the index of heat tolerant rapeseed ***,two heat insensitive rapeseed varieties with high oil content were identified.

关键词： Brassica napus L. heat stress seed filling stage oil accumulation chlorophyll fluorescence characteristics

Enhanced production of seed oil with improved fatty acid composition by overexpressing NAD^(+)-dependent glycerol-3-phosphate dehydrogenase in soybean

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Journal of Integrative Plant Biology 2021年第6期63卷 1036-1053页

作者： Ying Zhao Pan Cao Yifan Cui Dongxu Liu Jiapeng Li Yabin Zhao Siqi Yang Bo Zhang Runnan Zhou Minghao Sun Xuetian Guo Mingliang Yang Dawei Xin Zhanguo Zhang Xin Li Chen Lv Chunyan Liu Zhaoming Qi Jingyu Xu Xiaoxia Wu Qingshan Chen Department of Agriculture Northeast Agricultural UniversityHarbin 150000China Key Lab of Maize Genetics and Breeding Heilongjiang Academy of Agricultural SciencesHarbin 150000China Department of Agriculture Heilongjiang Bayi Agricultural UniversityDaqing 163319China

There is growing interest in expanding the production of soybean oils(mainly triacylglycerol, or TAG) to meet rising feed demand and address global energy concerns. We report that a plastidlocalized glycerol-3-phosphate dehydrogenase(GPDH), encoded by GmGPDHp1 gene, catalyzes the formation of glycerol-3-phosphate(G3 P), an obligate substrate required for TAG *** of GmGPDHp1 increases soybean seed oil content with high levels of unsaturated fatty acids(FAs), especially oleic acid(C18:1), without detectably affecting growth or seed protein content or seed weight. Based on the lipidomic analyses, we found that the increase in G3 P content led to an elevated diacylglycerol(DAG) pool, in which the Kennedy pathwayderived DAG was mostly increased, followed by PC-derived DAG, thereby promoting the synthesis of TAG containing relatively high proportion of C18:1. The increased G3 P levels induced several transcriptional alterations of genes involved in the glycerolipid pathways. In particular, genes encoding the enzymes responsible for de novo glycerolipid synthesis were largely upregulated in the transgenic lines, in-line with the identified biochemical phenotype. These results reveal a key role for GmGPDHp1-mediated G3 P metabolism in enhancing TAG synthesis and demonstrate a strategy to modify the FA compositions of soybean oils for improved nutrition and biofuel.

关键词： GmGPDHp1 G3P production lipidomics oil accumulation soybean transcriptomics

Transcriptional regulation of oil biosynthesis inseed plants: Current understanding, applications,and perspectives

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Plant Communications 2022年第5期3卷 19-37页

作者： Yuzhou Yang Que Kong Audrey R.Q.Lim Shaoping Lu Hu Zhao Liang Guo Ling Yuan Wei Ma School of Biological Sciences Nanyang Technological UniversitySingapore 637551Singapore National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China Department of Plant and Soil Sciences Kentucky Tobacco Research and Development CenterUniversity of KentuckyLexingtonKY 40546USA Guangdong Provincial Key Laboratory of Applied Botany South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China

Plants produce and accumulate triacylglycerol(TAG)in their seeds as an energy reservoir to support the processes of seed germination and seedling *** seed oils are vital not only for the human diet but also as renewable feedstocks for industrial *** biosynthesis consists of two major steps:de novo fatty acid biosynthesis in the plastids and TAG assembly in the endoplasmic *** latest advances in unraveling transcriptional regulation have shed light on the molecular mechanisms of plant oil *** summarize recent progress in understanding the regulatory mechanisms of wellcharacterized and newly discovered transcription factors and other types of regulators that control plant fatty acid *** emerging picture shows that plant oil biosynthesis responds to developmental and environmental cues that stimulate a network of interacting transcriptional activators and repressors,which in turn fine-tune the spatiotemporal regulation of the pathway genes.

关键词： plant oil biosynthesis oil accumulation seed development environmental and developmental signals transcription factor transcriptional regulation

A Study of the Accompanying Relationships between Uranium and oil

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Acta Geologica Sinica(English Edition) 2000年第3期74卷 595-601页

作者： LI Huaiyuan Airborne survey and Remote Sensing Center of Nuclear Industry Shijiazhuang 050002Hebei China University of Geosciences(Beijing) Xueyuan RoadBeijing100083

It is not occasional that uranium deposits and oil accumulation occur in the same depression in the Erlian basin, Inner Mongolia. Some evidences show certain relations between uranium and oil in origin. This paper discusses and analyses the evidence for the relations between uranium deposits and oil and gas accumulation in terms of spatial distribution, geology, hydrochemistry and geochemistry. The paper also deals with the mechanism of the formation of uranium deposits and points out that it is of significance to use uranium as an indicator to search for oil and vice versa.

关键词： Erlian basin uranium deposit oil accumulation accompanying relationship

Construction of a Normalized Full-Length cDNA Library of Sesame Developing Seed by DSN and SMART^(TM)

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Agricultural Sciences in China 2011年第7期10卷 1004-1009页

作者： KE Tao DONG Cai-hua MAO Han ZHAO Ying-zhong LIU Hong-yan LIU Sheng-yi Key Laboratory for oil Crops Biology Ministry of Agriculture/Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 P.R.China Department of Life Science and Technology Nanyang Normal University Nanyang 473061 P.R.China

Sesame （Sesamue indicum L.） is one of the most important oilseed crops with high oil yield. Here, we described a simple and efficient method for constructing a normalized cDNA library from a high oil content cultivar of sesame Zhongzhi 14, during its oil accumulation stages. It combined switching mechanism at 5？end of RNA transcript （SMART） technique and duplex-specific nuclease （DSN） normalization methods. Double-stranded cDNAs were synthesized from mRNAs, processed by normalization and Sfi I restriction endonuclease, and finally the cDNAs were ligated to pDNR-LIB vector. The ligation mixture was transformed into Escherichia coli DH10B by electroporation. The capacity of the library was 1.0？06 clones in this library. Gel electrophoresis results indicated the fragments ranged from 700 to 2 000 bp, with the average size of 1 800 bp. Random picking clones showed that the recombination rate was 100%. The results showed that the cDNA library constructed successfully was a full-length library with high quality, and could be used to screen the genes related to development of oil synthesis.

关键词： DSN full-length library normalization oil accumulation Sesamue indicum Zhongzhi 14 cDNA library switching mechanism SMARTTM

Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang

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Food Science and Human Wellness 2022年第5期11卷 1201-1214页

作者： Wenqiang Wang Hao Wen Qiang Jin Wenjun Yu Gen Li Minyu Wu Hongjin Bai Lirong Shen Cuiyun Wu Department of Food Science and Nutrition Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhou 310058China College of Horticulture and Forestry Tarim UniversityAlar 843300China

Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples at different development periods of three walnut cultivars,’Zhipi’(ZP),’Xinwu 417’(W417)and’Xinwen 81’(W81)were collected for transcriptomic *** analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70%,and over 90%of FAs were *** identified 126 candidate genes including 64 genes for FA de novo synthesis,45 genes for triacylglycerol assembly,and 17 genes for oil bodies involved in lipid biosynthesis by *** key enzymes including ACCase,LACS6,LACS8,SAD,FAD2,FAD3,LPAAT1,DGAT2,PDAT2,and PLC encoded by 19 genes were highly associated with lipid *** PCR analysis further validated 9 important genes,and the results were well consistent with our transcriptomic ***,5 important transcription factors including WRI1,ABI3,FUS3,PKL and VAL1 were identified,and their main regulatory genes might contain ACCase,KASII,LACS,FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut *** findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut.

关键词： Walnut oil accumulation Triacylglycerol assembly Transcription factors Correlation analysis

Petroleum exploration breakthrough and geological significance in Cretaceous Yingcheng and Denglouku formations of Shuangcheng area, northern Songliao Basin, NE China

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Petroleum Exploration and Development 2023年第1期50卷 72-84页

作者： LIU Chao FU Xiaofei LI Yangcheng WANG Haixue SUN Lidong LU Jiamin LI Junhui SUN Youhai SHI Lidong HU Huiting YUAN Hongqi YANG Zicheng Northeast Petroleum University Daqing 163318China Ministry of Education Key Laboratory of Continental Shale Hydrocarbon accumulation and Efficient Development Northeast Petroleum UniversityDaqing 163318China PetroChina Daqing oilfield Company Ltd. Daqing 163712China

Based on drilling geological,geochemical,geophysical and production test data,the characteristics of source rocks,reservoir rocks and caprocks,as well as the process of hydrocarbon generation,trap evolution and oil accumulation of the oil-bearing assemblage composed of the Cretaceous Yingcheng Formation(K1yc)and Denglouku Formation(K1d)in the Shuangcheng area,northern Songliao Basin,NE China,were analyzed by using the research methods for petroleum *** source rocks mainly exist in K1yc,with the organic matters mainly originated from lower aquatic organisms and algae,and reaching the grade of high-quality source rock in terms of organic *** crude oil,which is characterized by low density,high freezing point and high wax content,is believed to have generated by the K1yc source *** reservoir rocks include K1d sandstones and K1yc *** mudstone in the fourth member of K1d serves as the direct caprock of the oil *** oil was generated during the period between Yaojia Formation and Nenjiang Formation,and then accumulated during the periods of Nenjiang Formation and Paleogene–*** traps evolved in three stages:the late Yingcheng Formation,the late Quantou Formation and the late Nenjiang Formation,forming structural and structural-lithologic *** is concluded that good source-reservoir-caprock assemblage,late hydrocarbon charging,short migration distance and stable tectonic setting are favorable factors for the formation of oil reservoirs.

关键词： petroleum system Cretaceous Yingcheng Formation Cretaceous Denglouku Formation source rock reservoir oil accumulation Shuangcheng area Songliao Basin

Important Roles of Transcription Factors in Regulating Seed oil Biosynthesis to Increase Plant Storage Lipid Content

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Agricultural Science & Technology 2013年第1期14卷 30-34页

作者：沈奇韩宏仕秦信蓉向阳王仙萍赵跃赵云喻时周杜才富贵州省油菜研究所贵州贵阳550008

In this article, the biosynthetic pathways of storage oil accumulation in oilseed plants were briefly introduced, and the transcription factors, such as B3 do- main supeffamily genes, lecl gene, wril gene etc., and their important role in oil accumulation regulation was mainly elucidated. Overexpession of transcription factors as feasible ways of genetic manipulation to increase oJl content in oilseed crops are promising in a long-term perspective.

关键词： oil accumulation Transcription factors B3 domain superfamily Leafy cotyledon 1 (LEC1) Wrinkled1 (ERI1)