Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang

作     者：Wenqiang Wang Hao Wen Qiang Jin Wenjun Yu Gen Li Minyu Wu Hongjin Bai Lirong Shen Cuiyun Wu Wenqiang Wang;Hao Wen;Qiang Jin;Wenjun Yu;Gen Li;Minyu Wu;Hongjin Bai;Lirong Shen;Cuiyun Wu

作者机构：Department of Food Science and NutritionZhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhou 310058China College of Horticulture and ForestryTarim UniversityAlar 843300China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第5期

页      面：1201-1214页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by Major scientific and technological projects of Xinjiang Production and Construction Corps(2017DB006 and 2020KWZ-012) 

主　　题：Walnut Oil accumulation Triacylglycerol assembly Transcription factors Correlation analysis 

摘      要：Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples at different development periods of three walnut cultivars,’Zhipi’(ZP),’Xinwu 417’(W417)and’Xinwen 81’(W81)were collected for transcriptomic *** analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70%,and over 90%of FAs were *** identified 126 candidate genes including 64 genes for FA de novo synthesis,45 genes for triacylglycerol assembly,and 17 genes for oil bodies involved in lipid biosynthesis by *** key enzymes including ACCase,LACS6,LACS8,SAD,FAD2,FAD3,LPAAT1,DGAT2,PDAT2,and PLC encoded by 19 genes were highly associated with lipid *** PCR analysis further validated 9 important genes,and the results were well consistent with our transcriptomic ***,5 important transcription factors including WRI1,ABI3,FUS3,PKL and VAL1 were identified,and their main regulatory genes might contain ACCase,KASII,LACS,FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut *** findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut.