检索结果-南通市图书馆

transporters Contributing to Iron Trafficking in Plants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2011年第3期4卷 464-476页

作者： Sarah S. Conte Elsbeth L. Walker University of Massachusetts Amherst Biology Department 611 No. Pleasant St Amherst MA 01002 USA

This review will discuss recent progress in understanding the many roles of transporters in the whole-plant physiological processes that maintain iron （Fe） homeostasis. These processes include uptake from the soil via roots, control of transport from roots to above-ground parts of the plant, unloading of Fe from the xylem in above-ground parts, loading of Fe into mitochondria and plastids, transport of Fe to reproductive parts of the plant, and Fe mobilization during seed germination. In addition, we will discuss the mechanisms that plants use to cope with an apparently unintended conse- quence of Fe acquisition： the uptake of toxic heavy metals via Fe transporters. Rapid progress has been made in under- standing the transport processes involved in each of these areas in the last 5 years and this review will focus on this recent progress. We will also highlight the key questions regarding transport steps that remain to be elucidated.

关键词： nutrient and metal transport transporters gene regulation iron nicotianamine phytosiderophores.

The Oligopeptide transporters： A Small Gene Family with a Diverse Group of Substrates and Functions？

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2011年第3期4卷 407-415页

作者： Mark Lubkowit Department of Biology Saint Michael's College 1 Winooski Park Colchester VT 05439 USA

Genes in the Oligopeptide transport family encode integral membrane proteins that are believed to trans- locate their substrates from either the extracellular environment or an organelle into the cytosol. Phylogenetic analyses of plant transporters have revealed two distant clades. the Yellow Stripe-Like （YSL） proteins and the so-called Oligopeptide transporters （OPTs）, for which the family was named. Three categories of substrates have been identified for this family： small peptides, secondary amino acids bound to metals, and glutathione. Notably, the YSL transporters are involved in metal homeostasis through the translocation of metal-chelates, indicating a level of conservation both in biological func- tion as well as substrates. In contrast, the functions of OPT proteins seem to be less defined and, in this review, I will examine the supporting and contradictory evidence for the proposed roles of OPTs in such diverse functions as long-dis- tance sulfur distribution, nitrogen mobilization, metal homeostasis, and heavy metal sequestration through the transport of glutathione, metal-chelates, and peptides.

关键词： Molecular transport nutrient and metal transport transporters Oligopeptide transporters.

Comprehensive Sequence and Whole-Life-Cycle Expression Profile Analysis of the Phosphate transporter Gene Family in Rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2011年第6期4卷 1105-1122页

作者： Fang Liu Xiao-Jian Chang Ying Ye Wei-Bo Xie Ping Wu Xing-Ming Lian National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research （Wuhan） Nuazhong Agricultural University Wuhan430070 China State Key Laboratory of Plant Physiology and Biochemistry College of Life Science Zhejiang University Hangzhou 310058 China

Plant phosphate transporter （PT） genes comprise a large family with important roles in various physiological and biochemical processes. In this study, a database search yielded 26 potential PT family genes in rice （Oryza sativa）. Analysis of these genes led to identification of eight conserved motifs and 5-12 trans-membrane segments, most of them conserved. A total of 237 putative cis elements were found in the 2-kbupstream region of these genes. Of these, a majority were Pi-response and other stress-related cis regulatory elements, such as PHO-like, TATA-box-like, PHR1, or Helix-loop- helix elements, and WRKY1 and ABRE elements, suggesting gene regulation by these signals. Comprehensive expression analysis of these genes was performed using data from microarrays hybridized with RNA from 27 tissues covering the entire lifecycle from three rice genotypes： Minghui 63, Zhenshan 97, and Shanyou 63. Real-time PCR analysis confirmed that three rice PT genes are preferentially expressed in stamen at I d before flowering, two in panicle at the heading stage, and two in flag leaf at 14 d after the heading stage. Hormone-treatment experiments revealed differential up-regulation or down-regulation of 11 rice PT genes in seedlings exposed to five hormones, respectively. These results will be useful for elucidating the roles of these genes in the growth, development, and stress response of the rice plant.

关键词： molecular transport nutrient and metal transport nutrition gene expression molecular biology rice.

Post-Translational Regulation of AtFER2 Ferritin in Response to Intracellular Iron Trafficking during Fruit Development in Arabidopsis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2009年第5期2卷 1095-1106页

作者： Karl Ravet Brigitte Touraine Sun A. Kim Francoise Cellier Sebastien Thomine Mary Lou Guerinot Jean-Francois Briat Frederic Gaymard Biochimie et Physiologie Moleculaire des Plantes (B&PMP) Unite Mixte de Recherche CNRS INRA Universite Montpellier 2 SupAgro Place Viala Bat. 7 F-34060 Montpellier France Department of Biological Sciences Dartmouth College 6044 Gilman Hall Hanover NH 03755 USA Institut des Sciences du Vegetal CNRS Bat 23 Avenue de la Terrasse 91198 Gif-sur-Yvette France

Ferritins are major players in plant iron homeostasis. Surprisingly, their overexpression in transgenic plants led only to a moderate increase in seed iron content, suggesting the existence of control checkpoints for iron loading and storage in seeds. This work reports the identification of two of these checkpoints. First, measurement of seed metal content during fruit development in Arabidopsis thaliana reveals a similar dynamic of loading for Fe, Mn, Cu, and Zn. The step controlling metal loading into the seed occurs by the regulation of transport from the hull to the seed. Second, metal loading and ferritin abundance were monitored in different genetic backgrounds affected in vacuolar iron transport （AtVIT1, AtNRAMP3, AtNRAMP4） or plastid iron storage （AtFER1 to 4）. This approach revealed （1） a post-translational reg- ulation of ferritin accumulation in seeds, and （2） that ferritin stability depends on the balance of iron allocation between vacuoles and plastids. Thus, the success of ferritin overexpression strategies for iron biofortification, a promising approach to reduce iron-deficiency anemia in developing countries, would strongly benefit from the identification and engineering of mechanisms enabling the translocation of high amounts of iron into seed plastids.

关键词： nutrient and metal transport iron transport ferritin post-transcriptional control biofortification Arabidopsis.