检索结果-南通市图书馆

Characterization of long-term ex vivo expansion of tree shrew spermatogonial stem cells

Zoological Research 2023年第6期44卷 1080-1094页

作者： Cong Li Rui Bi Lin Wang Yu-Hua Ma Yong-Gang Yao Ping Zheng State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Key Laboratory of Animal Models and Human Disease Mechanisms of Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan 650204China National Resource Center for Non-Human Primates National Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650107China KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650204China

Tree shrews(Tupaia belangeri chinensis)share a close relationship to primates and have been widely used in biomedical *** previously established a spermatogonial stem cell(SSC)-based gene editing platform to generate transgenic tree ***,the influences of long-term expansion on tree shrew SSC spermatogenesis potential remain ***,we examined the in vivo spermatogenesis potential of tree shrew SSCs cultured across different *** found that SSCs lost spermatogenesis ability after long-term expansion(>50 passages),as indicated by the failure to colonize the seminiferous epithelium and generate donor spermatogonia(SPG)-derivedspermatocytesor spermatids marking *** sequencing(RNA-seq)analysis of undifferentiated SPGs across different passages revealed significant gene expression changes after sub-culturing primary SPG lines for more than 40 passages on feeder ***,DNA damage response and repair genes(e.g.,MRE11,SMC3,BLM,and GEN1)were down-regulated,whereas genes associated with mitochondrial function(e.g.,NDUFA9,NDUFA8,NDUFA13,and NDUFB8)were up-regulated after *** DNA damage accumulation and mitochondrial dysfunction were experimentally validated in high-passage *** with nicotinamide adenine dinucleotide(NAD+)precursor nicotinamide riboside(NR)exhibited beneficial effects by reducing DNA damage accumulation and mitochondrial dysfunction in SPG elicited by long-term *** research presents a comprehensive analysis of the genetic and physiological attributes critical for the sustained expansion of undifferentiated SSCs in tree shrews and proposes an effective strategy for extended in vitro maintenance.

关键词： Tree shrews Spermatogonial stem cells nicotinamide riboside DNA damage Mitochondrial dysfunction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NAD＋ and its precursors in human longevity

引用

Frontiers of Electrical and Electronic Engineering in China 2015年第4期10卷 193-198页

作者： Jimin Lin Yanchao Pan Jiangyun Wang Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Faculty of Medicine The Chinese University of Hong Kong Hong Kong SAR 999077 China

Aging is a complex issue due to its nature in progressive physiological and functional decay. As better medicine, technology, and living conditions became accessible to many people, the longevity of human beings increased during the past centuries. Recent research established vital roles for NAD＋ and its precursors in protecting and maintaining the redox homeostasis in cells, which might be applicable therapeutically to prevent cell degeneration. Notably, the contribution of NAD＋ metabolites to lifespan extension in model systems indicates that the potential beneficial effects of NAD＋ precursors. In this mini review, by introducing the background of NAD＋-consuming enzymes in ＂caloric restriction＂, we focus on NAD＋ and its precursors in diet, with further emphasis on its association with health and diseases. We also provide insights in future utilization ofNAD＋ and its precursors as nutrition supplement for lifespan extension.

关键词： longevity sirtuins NAD+ NAD+ synthesis NAD+ precursor nicotinamide riboside

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：